| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 723,528 | (T)8→9 | coding (887/2685 nt) | kdpD ← | fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 723,520 | 1 | . | T | 95.3% | 126.4 / ‑0.8 | 43 | coding (895/2685 nt) | kdpD | fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (22/19); total (23/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01 | |||||||||||
CCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATA > NC_000913/723387‑723651 | ccGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑‑tttt‑t > 2:2043044/1‑139 (MQ=255) ttGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAg < 1:2043044/139‑1 (MQ=255) ttGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAg < 1:210091/139‑1 (MQ=255) ttCACGGGCATAACCCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAATGCGCTGAGAATTGCCCGACG‑‑‑TTTT‑TTATTCCGGTAAGCGGCgcagggcagg < 2:1259647/139‑1 (MQ=255) cGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGtt < 2:546453/139‑1 (MQ=255) cGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGtt > 1:1852596/1‑139 (MQ=255) cGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGtt > 1:222325/1‑139 (MQ=255) gggCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGttt > 2:1989650/1‑139 (MQ=255) tAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACa > 2:1751110/1‑139 (MQ=255) cTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGTCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGtt < 2:280495/126‑1 (MQ=255) cTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAg > 2:687543/1‑108 (MQ=255) cTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAg < 1:687543/108‑1 (MQ=255) cTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGtt > 1:280495/1‑126 (MQ=255) tACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACcgc < 2:222325/139‑1 (MQ=255) gCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCCCCGCGCCCAGTTCCGGCGCCAGACGTAAGGCGCGGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGc < 1:2020235/139‑1 (MQ=255) cTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGt < 1:1780909/99‑1 (MQ=255) cTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGt > 2:1780909/1‑99 (MQ=255) cTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGttt > 2:1450907/1‑121 (MQ=255) cTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATCGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGttt < 1:1450907/121‑1 (MQ=255) cGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTAcc < 2:828837/139‑1 (MQ=255) aGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTTCCCAGCCGTGACGcc > 1:1808358/1‑139 (MQ=255) gtTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgc > 1:37717/1‑139 (MQ=255) ttGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgcc > 2:376732/1‑139 (MQ=255) ttGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGACAGACGCTACCCAGCCGTGACGCCAgcc > 2:1233692/1‑139 (MQ=255) gCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgccgc > 1:498339/1‑139 (MQ=255) ccGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgccgcg > 1:263749/1‑139 (MQ=255) cGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAg > 2:1542157/1‑71 (MQ=255) cGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAg < 1:1542157/71‑1 (MQ=255) tcCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGACTTGCCCGACG‑‑‑TTTTATT‑TTCCGGTAAGCGGTGCAGGGCAgggg > 1:1355318/1‑83 (MQ=255) tcCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAgggg < 2:1355318/83‑1 (MQ=255) ccAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGa > 2:1950551/1‑139 (MQ=255) aGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGAcc > 2:1407115/1‑139 (MQ=255) gTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTAGCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCa > 2:1289660/1‑139 (MQ=255) gcCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCg < 2:1852596/139‑1 (MQ=255) ccAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑‑tttt‑t > 2:1327743/1‑36 (MQ=255) ccAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑‑tttt‑t < 1:1327743/36‑1 (MQ=255) aGACGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTg > 1:1009553/1‑139 (MQ=255) aCGTAAGGCGCTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTCTTCGCTGcc < 2:1009553/139‑1 (MQ=255) cTGAGAATTGCCCGACGTTTTTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTAt > 2:1919012/1‑139 (MQ=255) cTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCa > 2:1225808/1‑55 (MQ=255) cTGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCa < 1:1225808/55‑1 (MQ=255) tGAGAATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGc < 2:37717/139‑1 (MQ=255) agaATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCg < 1:1950551/139‑1 (MQ=255) gaATTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACTCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGa < 1:1343612/139‑1 (MQ=255) aTTGCCCGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGGCCAGTTTTTCGCTGCCGGTGTTATGGCCGATg < 1:1989650/139‑1 (MQ=255) cccGACGT‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTCTGGCCGATGCATa > 2:1254138/1‑139 (MQ=255) | CCGAGAATAATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑‑TTTT‑TT‑TTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATA > NC_000913/723387‑723651 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |