| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,624,333 | (T)5→6 | coding (165/393 nt) | ydeI ← | hydrogen peroxide resistance OB fold protein, putative periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,624,328 | 1 | . | T | 98.6% | 219.8 / ‑4.1 | 69 | coding (170/393 nt) | ydeI | hydrogen peroxide resistance OB fold protein, putative periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (33/35); total (34/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTCCATCAAAGACGGCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GG‑TTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAT > NC_000913/1624199‑1624458 | cGTCCATCAAAGACGGCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GG‑ttttttgcg < 2:1521724/139‑5 (MQ=255)cGTCCATCAAAGACGGCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GG‑ttttttgcg > 1:897230/1‑135 (MQ=255) ccATCAAAGACGGCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGaaa > 2:1585483/1‑139 (MQ=255) tCAAAGACGGCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCa > 1:981179/1‑139 (MQ=255) cAAAGACGGCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCaa < 1:1653859/139‑1 (MQ=255) gCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCAtt > 1:895875/1‑139 (MQ=255) ccGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAAc > 2:629336/1‑131 (MQ=255) ccGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAAc < 1:629336/131‑1 (MQ=255) ccGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCAttt > 2:107259/1‑139 (MQ=255) gAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCAtttttttgc < 2:513871/139‑3 (MQ=255) ttAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTc > 1:688548/1‑139 (MQ=255) aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAg < 2:981179/139‑1 (MQ=255) aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAg < 2:688548/139‑1 (MQ=255) aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAg < 2:897230/139‑1 (MQ=255) cGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGcc < 2:1619005/139‑1 (MQ=255) tctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAtc < 2:976596/139‑1 (MQ=255) tctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAtc < 2:895875/139‑1 (MQ=255) tctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAtc < 2:273178/139‑1 (MQ=255) ctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAtct > 2:2049978/1‑139 (MQ=255) aTCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGcc > 1:142039/1‑132 (MQ=255) aTCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGcc < 2:142039/132‑1 (MQ=255) aaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCACTTTTTTTGCATCATCGGt > 1:310183/1‑113 (MQ=255) aaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCACTTTTTTTGCATCATCGGt < 2:310183/113‑1 (MQ=255) gTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAggc < 1:1341416/139‑1 (MQ=255) tCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAt > 1:28222/1‑115 (MQ=255) tCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAt < 2:28222/115‑1 (MQ=255) tCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTg < 2:771290/139‑1 (MQ=255) tCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTg > 1:881647/1‑139 (MQ=255) tctcCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCAtttttttgc < 2:1329269/89‑3 (MQ=255) tctcCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCAtttttttgc > 1:1636264/1‑87 (MQ=255) tctcCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCAtttttttgc > 1:1329269/1‑87 (MQ=255) tctcCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCAtttttttgc < 2:1636264/89‑3 (MQ=255) tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc < 2:1839468/139‑1 (MQ=255) tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc < 2:313720/139‑1 (MQ=255) tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc > 1:1839468/1‑139 (MQ=255) agaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACgg > 1:1679726/1‑66 (MQ=255) agaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACgg < 2:1679726/66‑1 (MQ=255) agaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCt < 1:1362950/130‑1 (MQ=255) agaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCt > 2:1362950/1‑130 (MQ=255) aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat < 1:1381236/80‑1 (MQ=255) aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat > 2:1381236/1‑80 (MQ=255) aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGAt > 2:649330/1‑92 (MQ=255) aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGAt < 1:649330/92‑1 (MQ=255) aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGtttt > 1:1983472/1‑139 (MQ=255) aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGtttt > 2:1920412/1‑139 (MQ=255) aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGtttt > 1:15430/1‑139 (MQ=255) aTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTa < 2:15430/139‑1 (MQ=255) tACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGcc < 1:1920412/139‑1 (MQ=255) ccGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCt > 1:328282/1‑139 (MQ=255) cgTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAg > 1:1815547/1‑81 (MQ=255) cgTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAg < 2:1815547/81‑1 (MQ=255) cgTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGt > 2:121922/1‑139 (MQ=255) cgTAGTGAAACCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGt > 1:1242378/1‑139 (MQ=255) aaaCCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGa < 1:1238443/92‑1 (MQ=255) aaaCCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGa > 2:1238443/1‑92 (MQ=255) aCCGAAGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa < 2:328282/139‑1 (MQ=255) aaGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCAttttttt > 1:1042410/1‑47 (MQ=38) aaGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCAttttttt < 2:1042410/47‑1 (MQ=38) aaGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGc > 1:381535/1‑139 (MQ=255) aaGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGc > 1:644116/1‑139 (MQ=255) aGCGCCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa < 2:1242378/139‑1 (MQ=255) gcgcCATCGTGCATAGT‑TTTTTGCGAAATCAACGGTCa > 2:1250691/1‑38 (MQ=25) gcgcCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCa < 1:1250691/38‑1 (MQ=37) gcgcCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat > 2:1164281/1‑52 (MQ=37) gcgcCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat < 1:1164281/52‑1 (MQ=37) gcgcCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCAt > 2:697456/1‑139 (MQ=255) gcCATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATaa < 1:2049978/139‑1 (MQ=255) cATCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAgg > 2:1566429/1‑139 (MQ=255) aTCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc < 1:95101/99‑1 (MQ=255) aTCGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc > 2:95101/1‑99 (MQ=255) cGTGCAT‑GGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt < 2:881647/139‑1 (MQ=255) | CGTCCATCAAAGACGGCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCAT‑GG‑TTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAT > NC_000913/1624199‑1624458 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |