| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,818,550 | (C)7→8 | intergenic (‑50/+55) | chbF ← / ← chbR | phospho‑chitobiase, general 6‑phospho‑beta‑glucosidase activity/repressor of chb operon for N,N'‑diacetylchitobiose utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,818,543 | 1 | . | C | 98.6% | 243.6 / ‑3.7 | 69 | intergenic (‑43/+62) | chbF/chbR | phospho‑chitobiase, general 6‑phospho‑beta‑glucosidase activity/repressor of chb operon for N,N'‑diacetylchitobiose utilization |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (34/34); total (34/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTA > NC_000913/1818414‑1818668 | gTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑ccc‑cccccg < 2:1542956/139‑3 (MQ=255) tCCTTCCAGTAACTCCGGGGTATAGCTGCTCCTGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCa > 1:1548169/1‑139 (MQ=255) tCCTTCCAGTAACTACGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCa > 2:493668/1‑139 (MQ=255) cTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAAc < 2:1804490/139‑1 (MQ=255) tCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAg > 2:1076746/1‑139 (MQ=255) cAGTAACTCCGGGGTATAGCTGCTCCTGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑CCCACCCCGGACATAAATAATCCAGCAACAGGa < 2:1548169/139‑1 (MQ=255) cAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa < 1:1076746/139‑1 (MQ=255) cAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa < 2:1123914/139‑1 (MQ=255) cAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa < 2:1598875/139‑1 (MQ=255) cAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa < 2:1805913/139‑1 (MQ=255) cAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa < 2:1895382/139‑1 (MQ=255) cGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATAtgt > 1:1370405/1‑139 (MQ=255) aGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGt < 2:1182912/139‑1 (MQ=255) aGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCaa < 2:972234/117‑1 (MQ=255) aGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCaa > 1:972234/1‑117 (MQ=255) aGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑ccc‑cccccggac < 2:1471896/101‑6 (MQ=255) aGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATAACTGGCATATCTG‑‑ccc‑cccccggac > 1:1471896/1‑96 (MQ=255) gctgctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGAACAGATATGTGAATTGTCa < 2:1370405/139‑1 (MQ=255) gctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGAc < 1:330090/119‑1 (MQ=255) gctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGAc > 2:330090/1‑119 (MQ=255) ctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACACAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGta > 1:548389/1‑139 (MQ=255) cccGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATcc > 2:638431/1‑105 (MQ=255) cccGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATcc < 1:638431/105‑1 (MQ=255) cccGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGa < 2:884122/119‑1 (MQ=255) cccGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGa > 1:884122/1‑119 (MQ=255) acgacTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCa < 1:2008223/117‑1 (MQ=255) acgacTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCa > 2:2008223/1‑117 (MQ=255) acTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGt < 1:1931059/113‑1 (MQ=255) acTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGt > 2:1931059/1‑113 (MQ=255) acTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCaa > 1:1333212/1‑91 (MQ=255) acTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCaa < 2:1333212/91‑1 (MQ=255) aTTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACACAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCaa < 2:548389/139‑1 (MQ=255) cTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAATAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTc > 1:11338/1‑139 (MQ=255) cTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑ccc‑ccc < 2:1705995/58‑1 (MQ=255) cTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑ccc‑ccc > 1:1705995/1‑58 (MQ=255) tGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa > 1:1356619/1‑84 (MQ=255) tGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa < 2:1356619/84‑1 (MQ=255) tGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCt > 2:1409178/1‑139 (MQ=255) tGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑ccc‑cccc > 1:887936/1‑58 (MQ=255) tGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATAGCTGGCATATCTG‑‑ccc‑cccc < 2:887936/58‑1 (MQ=255) aaTTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGAAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAg < 1:1409178/139‑1 (MQ=255) tcCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCaa < 2:430750/65‑1 (MQ=255) tcCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCaa > 1:430750/1‑65 (MQ=255) ttCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGAGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCg > 2:1339732/1‑139 (MQ=255) tCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATcc < 2:46736/55‑1 (MQ=255) tCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATcc > 1:46736/1‑55 (MQ=255) tCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa > 1:818237/1‑65 (MQ=255) tCAGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa < 2:818237/65‑1 (MQ=255) ggTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGAGATAAATAATCCAGCAACAGGa > 2:1263316/2‑63 (MQ=255) aGTACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa < 1:1263316/63‑1 (MQ=255) tACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGAt > 1:2033209/1‑122 (MQ=255) tACAGAATACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGAt < 2:2033209/122‑1 (MQ=255) aaTACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAg > 1:183509/1‑54 (MQ=255) aaTACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAg < 2:183509/54‑1 (MQ=255) aaTACTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGt > 1:295036/1‑139 (MQ=255) aCTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa < 1:803845/53‑1 (MQ=255) aCTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa > 2:803845/1‑53 (MQ=255) aCTGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTaa > 2:1003463/1‑139 (MQ=255) tGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGa > 1:1527366/1‑111 (MQ=255) tGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGa < 2:1527366/111‑1 (MQ=255) tGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCtt < 1:1596829/119‑1 (MQ=255) tGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCtt > 2:1596829/1‑119 (MQ=255) tGATATCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAAtt > 1:259548/1‑139 (MQ=255) atatCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCaa < 1:1382188/44‑1 (MQ=255) atatCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCaa > 2:1382188/1‑44 (MQ=255) atatCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATAtgt > 1:1886103/1‑58 (MQ=255) atatCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATAtgt < 2:1886103/58‑1 (MQ=255) atatCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCa > 1:1129012/1‑67 (MQ=255) atatCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCa < 2:1129012/67‑1 (MQ=255) tCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACGGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTa < 2:11338/139‑1 (MQ=255) tCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa > 2:1450976/1‑46 (MQ=255) tCTGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa < 1:1450976/46‑1 (MQ=255) tGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGc < 2:59780/37‑1 (MQ=255) tGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGc > 1:59780/1‑37 (MQ=255) tGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa < 2:749020/44‑1 (MQ=255) tGGCATATCTGC‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGa > 1:749020/1‑44 (MQ=255) | GTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑CCC‑CCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTA > NC_000913/1818414‑1818668 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |