BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,664,363	(G)_6→7	coding (256/729 nt)	gadW ←	transcriptional activator of gadA and gadBC, repressor of gadX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,664,357	1	.	G	98.7%	539.2 / ‑1.4	151	coding (262/729 nt)	gadW	transcriptional activator of gadA and gadBC, repressor of gadX
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (70/79); total (71/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 185 total aligned reads displayed.

TGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑GG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACA  >  NC_000913/3664219‑3664489

tGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                                         <  2:484324/139‑1 (MQ=255)

   tATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gg‑g                                                                                                                                    <  2:1240676/139‑1 (MQ=255)

    aTTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                                         >  2:1288682/1‑135 (MQ=255)

    aTTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gg‑gg                                                                                                                                   >  2:1845144/1‑139 (MQ=255)

    aTTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCACGGATTCATTGAAGCTATAAAAAATCGCCGTCACCAGCGGCAT‑gg‑gg                                                                                                                                   >  1:427934/1‑139 (MQ=255)

          aaGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gg‑ggg‑ggtga                                                                                                                            <  1:371536/139‑5 (MQ=255)

               aGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                                         <  1:780321/124‑1 (MQ=255)

               aGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGt                                                                                                                        <  1:379400/139‑1 (MQ=255)

               aGGGGATCAGTTCTTTCTTATAAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGt                                                                                                                        >  2:1827528/1‑139 (MQ=255)

                      cAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGt                                                                                                                        >  1:1387935/1‑132 (MQ=255)

                       aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                                         <  1:623173/116‑1 (MQ=255)

                       aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gg‑ggg‑ggtgac                                                                                                                           >  2:1590984/1‑122 (MQ=255)

                       aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gg‑ggg‑ggtgac                                                                                                                           <  1:1590984/127‑6 (MQ=255)

                       aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gg‑g                                                                                                                                    >  1:703781/1‑119 (MQ=255)

                         ttctttctTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACaa                                                                                                              <  1:604126/139‑1 (MQ=255)

                           ctttcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAAc                                                                                                            <  2:1557417/139‑1 (MQ=255)

                            tttcttATGAGAAAACATCGATAAAGAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACg                                                                                                           >  2:754253/1‑139 (MQ=255)

                              tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGt                                                                                                         <  1:1845144/139‑1 (MQ=255)

                              tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCGCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑GGCGGG‑GTGACTGGTTAATTAACAAACGGt                                                                                                         >  1:1187082/1‑139 (MQ=255)

                                 tATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCtt                                                                                                      >  1:1982808/1‑139 (MQ=255)

                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCGTTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGtt                                                                                                                       <  2:1727348/120‑1 (MQ=255)

                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCttta                                                                                                    <  1:1833257/139‑2 (MQ=255)

                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    <  1:1036771/139‑1 (MQ=255)

                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTACTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gg                                                                                                                                      <  1:1075338/106‑1 (MQ=255)

                                        aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCATCAGCGGCAT‑GG‑GGGCGTGACCGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:1331364/139‑1 (MQ=255)

                                        aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  2:762690/139‑1 (MQ=255)

                                        aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:1289319/139‑1 (MQ=255)

                                         aaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:1151768/138‑1 (MQ=255)

                                              cGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAAccc                                                                                         >  1:33666/1‑139 (MQ=255)

                                                        gAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGt                                                                               <  1:743200/139‑1 (MQ=255)

                                                             gTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                          <  2:1922661/139‑1 (MQ=255)

                                                             gTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                          <  2:213042/139‑1 (MQ=255)

                                                               agagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCt                                                                        <  1:1248517/139‑1 (MQ=255)

                                                                gagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:808038/115‑1 (MQ=255)

                                                                gagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTg                                                                       >  1:1942768/1‑139 (MQ=255)

                                                                gagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTg                                                                       >  1:1283126/1‑139 (MQ=255)

                                                                 agCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAATAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGa                                                                      <  2:1343899/139‑1 (MQ=255)

                                                                  gCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  2:1080328/113‑1 (MQ=255)

                                                                  gCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGAc                                                                     >  2:1870785/1‑139 (MQ=255)

                                                                     tatTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGaa                                                                  <  1:1733617/139‑1 (MQ=255)

                                                                      atTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    >  2:1127314/1‑104 (MQ=255)

                                                                       ttGCTCAGTATCGGTGACTTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAata                                                                >  1:1673968/1‑139 (MQ=255)

                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACaa                                                                                                              <  1:2014764/93‑1 (MQ=255)

                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACaa                                                                                                              >  2:2014764/1‑93 (MQ=255)

                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGt                                                                                                         >  2:1152835/1‑98 (MQ=255)

                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAata                                                                >  1:734691/1‑139 (MQ=255)

                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAata                                                                >  1:906504/1‑139 (MQ=255)

                                                                        tGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAatat                                                               >  2:1552817/1‑139 (MQ=255)

                                                                           tCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               >  2:977104/1‑104 (MQ=255)

                                                                           tCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:977104/104‑1 (MQ=255)

                                                                             aGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgt                                                          <  2:425432/139‑1 (MQ=255)

                                                                               tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               >  1:1922705/1‑100 (MQ=255)

                                                                               tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                          >  1:1821058/1‑121 (MQ=255)

                                                                               tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATAtt                                                              >  1:1514333/1‑133 (MQ=255)

                                                                                aTCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgtaag                                                       <  2:906504/139‑1 (MQ=255)

                                                                                  cGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               >  2:1934054/1‑97 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACaa                                                                                                              <  2:1911954/80‑1 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACaa                                                                                                              >  1:1911954/1‑80 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    <  2:103732/90‑1 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    <  1:841948/90‑1 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    <  1:759007/90‑1 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    >  2:841948/1‑90 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:1899754/95‑1 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                          >  1:424523/1‑116 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAAt                                                   >  2:1015439/1‑139 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAAt                                                   >  1:1055234/1‑139 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gg‑ggg‑ggtga                                                                                                                            >  2:1415416/1‑61 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gg‑ggg                                                                                                                                  >  2:886848/1‑60 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gg‑ggg                                                                                                                                  <  1:886848/60‑1 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    >  2:1071480/1‑85 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTg                                                           <  1:23262/126‑1 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTg                                                           >  2:23262/1‑126 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGc                                                <  2:1992681/137‑1 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGc                                                >  1:1992681/1‑137 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCaa                                              >  1:1011285/1‑139 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gg‑gg                                                                                                                                   <  2:374797/54‑1 (MQ=255)

                                                                                                 ggATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    <  1:1119601/77‑1 (MQ=255)

                                                                                                 ggATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAg                                      >  2:805001/1‑139 (MQ=255)

                                                                                                 ggATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gg‑ggg‑ggt                                                                                                                              <  1:929806/50‑3 (MQ=255)

                                                                                                 ggATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gg‑ggg‑ggt                                                                                                                              >  2:929806/1‑48 (MQ=255)

                                                                                                  gATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                          <  1:476650/102‑1 (MQ=255)

                                                                                                  gATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGc                                     >  2:694071/1‑139 (MQ=255)

                                                                                                   aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGttaattaa                                                                                                                 <  1:1426396/62‑1 (MQ=255)

                                                                                                   aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGttaattaa                                                                                                                 >  2:1426396/1‑62 (MQ=255)

                                                                                                   aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gg‑ggg                                                                                                                                  <  2:859636/45‑1 (MQ=255)

                                                                                                     tCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCa                                  <  1:1870785/139‑1 (MQ=255)

                                                                                                      cATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCAt                                 <  2:275287/139‑1 (MQ=255)

                                                                                                        ttGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCg                               <  2:504114/139‑1 (MQ=255)

                                                                                                        ttGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCg                               <  2:1982808/139‑1 (MQ=255)

                                                                                                            aGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gg‑ggg‑ggtga                                                                                                                            <  2:733571/41‑5 (MQ=255)

                                                                                                              cTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               >  1:1261197/1‑69 (MQ=255)

                                                                                                              cTATCAAAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  2:1261197/69‑1 (MQ=255)

                                                                                                                 tCACAAATCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgtaagta                                                     <  2:1319867/108‑1 (MQ=255)

                                                                                                                    aaaaTCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgtcgt                   <  2:1283126/139‑1 (MQ=255)

                                                                                                                      aaTCGCCGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    >  1:861090/1‑56 (MQ=255)

                                                                                                                           ccGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAg                                      >  2:985972/1‑113 (MQ=255)

                                                                                                                           ccGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAg                                      <  1:985972/113‑1 (MQ=255)

                                                                                                                           ccGTCACCAGCGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTAt            <  1:1417791/139‑1 (MQ=255)

                                                                                                                                     cGGCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACa  <  2:1911366/139‑1 (MQ=255)

                                                                                                                                      ggCATGGG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgt                      >  2:456215/1‑118 (MQ=255)

TGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑GG‑GGG‑GTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACA  >  NC_000913/3664219‑3664489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`