Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,886,810	G→A	*322* (TAG→TAA)	yeaX →	putative YeaWX dioxygenase beta subunit, reductase component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,886,810	0	G	A	100.0%	218.7 / NA	68	*322* (TAG→TAA)	yeaX	putative YeaWX dioxygenase beta subunit, reductase component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/33);  total (35/33)

GAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCG  >  NC_000913/1886694‑1886940                                                                                                                      |                                                                                                                                   gAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGgcg                                                                                                              >  1:1829710/1‑139 (MQ=255)  aaGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGgcgc                                                                                                             <  2:1829710/139‑1 (MQ=255)      tGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGcc                                                                                                                        >  1:293265/1‑124 (MQ=255)      tGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGcc                                                                                                                        <  2:293265/124‑1 (MQ=255)      tGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAg                                                                                                         >  1:931429/1‑139 (MQ=255)                        tCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTATTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTc                                                                                       >  1:1753496/1‑139 (MQ=255)                                    cGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGgcgc                                                                                                             <  2:23043/105‑1 (MQ=255)                                    cGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGgcgc                                                                                                             >  1:23043/1‑105 (MQ=255)                                    cGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTAc                                                                           >  2:1188423/1‑139 (MQ=255)                                     gATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACt                                                                          <  1:1188423/139‑1 (MQ=255)                                      aTGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTg                                                                         >  2:1755020/1‑139 (MQ=255)                                         aaGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCgg                                                                      >  2:1126346/1‑139 (MQ=255)                                              cGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGc                                                                                                                         >  1:1383807/1‑83 (MQ=255)                                              cGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGc                                                                                                                         <  2:1383807/83‑1 (MQ=255)                                                tGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGcc                                                                                                                              >  2:1280350/1‑76 (MQ=255)                                                tGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGcc                                                                                                                              <  1:1280350/76‑1 (MQ=255)                                                 gccagccaGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACt                                                                          >  2:484992/1‑127 (MQ=255)                                                 gccagccaGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACt                                                                          <  1:484992/127‑1 (MQ=255)                                                 gccagccaGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCt                                                              <  2:1698477/139‑1 (MQ=255)                                                   cagccagcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCgg                                                                                                                      >  2:452732/1‑81 (MQ=255)                                                   cagccagcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCgg                                                                                                                      <  1:452732/81‑1 (MQ=255)                                                     ttaggagcaggaTGTTGTTCGTTTGTCGGCGGGCAAGGGTT‑AACCGCTTGGTTTGGGATTTTTAATTTGCCAAACCGGGTTGTGGGCCTGGGCGCGCCAAGCCGGGCTTGGGAAGTTTTTATTG‑GGATATTCCTGCAAt                                                         <  2:781794/128‑1 (MQ=255)                                                      ccagcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCg                                                                                                                             <  1:543112/71‑1 (MQ=255)                                                      ccagcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCg                                                                                                                             >  2:543112/1‑71 (MQ=255)                                                        agcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTAc                                                                           <  2:1945439/119‑1 (MQ=255)                                                        agcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTAc                                                                           >  1:1945439/1‑119 (MQ=255)                                                          cAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGAtgt                                                                                                                  >  1:1985073/1‑78 (MQ=255)                                                          cAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGAtgt                                                                                                                  <  2:1985073/78‑1 (MQ=255)                                                          cAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGAAGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAAt                                                     >  1:1321149/1‑139 (MQ=255)                                                                aTGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTa                                               >  1:1618116/1‑139 (MQ=255)                                                                    tGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGt                                                                                               <  2:1781141/87‑1 (MQ=255)                                                                    tGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGt                                                                                               >  1:1781141/1‑87 (MQ=255)                                                                      aTCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCtt                                         <  1:1126346/139‑1 (MQ=255)                                                                       tCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTc                                        >  2:1992995/1‑139 (MQ=255)                                                                         tgttgtTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGcc                                                                                                   <  1:1900615/78‑1 (MQ=255)                                                                         tgttgtTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGcc                                                                                                   >  2:1900615/1‑78 (MQ=255)                                                                         tgttgtTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTc                                                                >  2:1018247/1‑113 (MQ=255)                                                                         tgttgtTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTc                                                                <  1:1018247/113‑1 (MQ=255)                                                                           ttgttCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTg                                                                                                           >  1:1614849/1‑68 (MQ=255)                                                                           ttgttCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTg                                                                                                           <  2:1614849/68‑1 (MQ=255)                                                                           ttgttCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTc                                                                >  2:1424851/1‑111 (MQ=255)                                                                           ttgttCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTc                                                                <  1:1424851/111‑1 (MQ=255)                                                                           ttgttCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGG‑TATTCCTGCAATAAATTGGGTaactggctcttat                                   >  1:781794/1‑128 (MQ=255)                                                                             gttCGCGTGAGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCAt                                  <  2:1321149/139‑1 (MQ=255)                                                                                cgcgTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACt                                                                          <  1:445273/96‑1 (MQ=255)                                                                                cgcgTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACt                                                                          >  2:445273/1‑96 (MQ=255)                                                                                cgcgTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAg                               <  2:676003/139‑1 (MQ=255)                                                                                     gCGAAGGGTAAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCg                                                                                      >  1:646504/1‑80 (MQ=255)                                                                                     gCGAAGGGTAAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCg                                                                                      <  2:646504/80‑1 (MQ=255)                                                                                     gCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGAt                                                                                                                    >  1:1146046/1‑49 (MQ=255)                                                                                     gCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGAt                                                                                                                    <  2:1146046/49‑1 (MQ=255)                                                                                         aGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACg                      <  2:1618116/139‑1 (MQ=255)                                                                                               aaaCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGc                 <  2:1753496/139‑1 (MQ=255)                                                                                                aaCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGcc                                                                                                   <  1:882815/56‑1 (MQ=255)                                                                                                aaCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGcc                                                                                                   >  2:882815/1‑56 (MQ=255)                                                                                                aaCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGcc                >  2:1111732/1‑139 (MQ=255)                                                                                                 aCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCCGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGGCAGTCg               <  1:1992995/139‑1 (MQ=255)                                                                                                      tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGcc                                                                                                   <  2:837529/50‑1 (MQ=255)                                                                                                      tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGcc                                                                                                   >  1:837529/1‑50 (MQ=255)                                                                                                      tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGa                                                                     >  1:436183/1‑80 (MQ=255)                                                                                                      tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGa                                                                     <  2:436183/80‑1 (MQ=255)                                                                                                      tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAAt          >  2:1517550/1‑139 (MQ=255)                                                                                                       ggTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATc         >  2:1201116/1‑139 (MQ=255)                                                                                                        gtgtTGGATTTGTAATTTGCCGAAGCCGGATGTGgcgc                                                                                                             >  1:91020/1‑38 (MQ=255)                                                                                                        gtgtTGGATTTGTAATTTGCCGAAGCCGGATGTGgcgc                                                                                                             <  2:91020/38‑1 (MQ=255)                                                                                                        gtgtTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCg                                                                       >  1:1668719/1‑76 (MQ=255)                                                                                                        gtgtTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCg                                                                       <  2:1668719/76‑1 (MQ=255)                                                                                                              gATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCg  <  1:1755020/139‑1 (MQ=255)                                                                                                                      |                                                                                                                                   GAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCG  >  NC_000913/1886694‑1886940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑