Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,238,562	(T)7→6	intergenic (+265/‑18)	ygjR → / → alx	putative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,238,556	0	T	.	100.0%	183.3 / NA	44	intergenic (+259/‑24)	ygjR/alx	putative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (18/26);  total (18/26)

TGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCA  >  NC_000913/3238418‑3238678                                                                                                                                           |                                                                                                                           tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACGTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                            >  1:1099552/1‑139 (MQ=255) tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                            >  1:493265/1‑139 (MQ=255) tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                            >  1:642626/1‑139 (MQ=255) tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                            >  1:330093/1‑139 (MQ=255) tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                            >  2:840860/1‑139 (MQ=255)    gAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACATTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttt                                                                                                                         >  2:117825/1‑139 (MQ=255)      aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                       <  2:493265/139‑1 (MQ=255)      aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                       <  1:840860/139‑1 (MQ=255)           cacaTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                       <  2:1021325/134‑1 (MQ=255)           cacaTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                       >  1:1021325/1‑134 (MQ=255)                ccGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAgg                                                                                                            <  2:642626/139‑1 (MQ=255)                  ggTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGaa                                                                                                          <  2:485832/139‑1 (MQ=255)                  ggTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGaa                                                                                                          <  2:330093/139‑1 (MQ=255)                  ggTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGaa                                                                                                          <  2:912224/139‑1 (MQ=255)                    tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttta                                                                                                                      <  2:312047/126‑2 (MQ=255)                    tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttta                                                                                                                      >  1:312047/1‑125 (MQ=255)                    tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACt                                                                                                        <  1:988307/139‑1 (MQ=255)                    tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACt                                                                                                        <  1:117825/139‑1 (MQ=255)                    tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACt                                                                                                        <  1:434294/139‑1 (MQ=255)                    tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCAGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACt                                                                                                        <  2:1099552/139‑1 (MQ=255)                        cGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTa                                                                                                    >  1:510690/1‑139 (MQ=255)                                       gAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                     <  1:338219/139‑1 (MQ=255)                                          tACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCAc                                                                                    <  2:62542/137‑1 (MQ=255)                                          tACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCAc                                                                                    >  1:62542/1‑137 (MQ=255)                                          tACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGc                                                                                  <  2:355736/139‑1 (MQ=255)                                           aCGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGcc                                                                                 <  2:1095150/139‑1 (MQ=255)                                            cGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                <  1:833454/139‑1 (MQ=255)                                                  cGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTa                                                                          >  1:113155/1‑139 (MQ=255)                                                      ttCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                       >  2:391548/1‑91 (MQ=255)                                                      ttCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                       <  1:391548/91‑1 (MQ=255)                                                      ttCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATggg                                                                      >  1:129312/1‑139 (MQ=255)                                                           tttttGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGa                                                                 >  2:531947/1‑139 (MQ=255)                                                                         gagaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgttgt                                                   <  2:113155/139‑1 (MQ=255)                                                                            aCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttttat                                                                                                                     <  1:239486/71‑3 (MQ=255)                                                                            aCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttttat                                                                                                                     >  2:239486/1‑69 (MQ=255)                                                                              cTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCa                                              >  1:22232/1‑139 (MQ=255)                                                                                  ccGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGaa                                                                                                          <  1:627316/75‑1 (MQ=255)                                                                                  ccGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGaa                                                                                                          >  2:627316/1‑75 (MQ=255)                                                                                       aGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgttgt                                                   >  1:460841/1‑125 (MQ=255)                                                                                       aGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgttgt                                                   <  2:460841/125‑1 (MQ=255)                                                                                          cGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttttat                                                                                                                     >  1:496001/1‑55 (MQ=255)                                                                                          cGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttttat                                                                                                                     <  2:496001/57‑3 (MQ=255)                                                                                            aGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATc                                <  2:129312/139‑1 (MQ=255)                                                                                                   tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTc                                                                                                      <  2:215256/62‑1 (MQ=255)                                                                                                   tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTc                                                                                                      >  1:215256/1‑62 (MQ=255)                                                                                                   tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGa                              <  2:580142/134‑1 (MQ=255)                                                                                                   tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGa                              >  1:580142/1‑134 (MQ=255)                                                                                                        aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGa                                                                                                 <  1:361216/62‑1 (MQ=255)                                                                                                        aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGa                                                                                                 >  2:361216/1‑62 (MQ=255)                                                                                                        aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGttgttg                    >  2:389806/1‑139 (MQ=255)                                                                                                         aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                       >  2:944299/1‑40 (MQ=255)                                                                                                         aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                       <  1:944299/40‑1 (MQ=255)                                                                                                         aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTc                                                                                                      <  2:740942/56‑1 (MQ=255)                                                                                                         aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTc                                                                                                      >  1:740942/1‑56 (MQ=255)                                                                                                           aaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATgggg                                                                     <  1:595968/87‑1 (MQ=255)                                                                                                           aaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATgggg                                                                     >  2:595968/1‑87 (MQ=255)                                                                                                               agcGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgtt                                                        <  2:542787/96‑1 (MQ=255)                                                                                                               agcGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgtt                                                        >  1:542787/1‑96 (MQ=255)                                                                                                                gcGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGcg            >  1:570704/1‑139 (MQ=255)                                                                                                                 cGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGaa                                                                                                          <  1:436825/44‑1 (MQ=38)                                                                                                                 cGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGaa                                                                                                          >  2:436825/1‑44 (MQ=38)                                                                                                                      gACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACg                                                                                   <  1:217090/62‑1 (MQ=255)                                                                                                                      gACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACg                                                                                   >  2:217090/1‑62 (MQ=255)                                                                                                                          tCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca  <  1:377005/139‑1 (MQ=255)                                                                                                                                           |                                                                                                                           TGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCA  >  NC_000913/3238418‑3238678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑