| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,818,550 | (C)7→8 | intergenic (‑50/+55) | chbF ← / ← chbR | phospho‑chitobiase, general 6‑phospho‑beta‑glucosidase activity/repressor of chb operon for N,N'‑diacetylchitobiose utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,818,543 | 1 | . | C | 97.7% | 153.3 / ‑3.5 | 44 | intergenic (‑43/+62) | chbF/chbR | phospho‑chitobiase, general 6‑phospho‑beta‑glucosidase activity/repressor of chb operon for N,N'‑diacetylchitobiose utilization |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (21/22); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTAA > NC_000913/1818412‑1818669 | tCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTCTAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑cc‑ccccc < 2:680189/139‑1 (MQ=255) cTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACataaat > 1:773163/1‑139 (MQ=255) aTCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGc < 1:931835/139‑1 (MQ=255) tCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCa > 2:926744/1‑139 (MQ=255) ttCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTGCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACa < 2:773163/139‑1 (MQ=255) aGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGAc < 1:926744/139‑1 (MQ=255) cGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGa < 1:2095/130‑1 (MQ=255) cGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGa > 2:2095/1‑130 (MQ=255) ggggTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑cc‑ccccccggac < 2:1110907/108‑6 (MQ=255) ggggTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑cc‑ccccccggac > 1:1110907/1‑103 (MQ=255) gggTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATcc > 2:938/1‑118 (MQ=255) gggTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATcc < 1:938/118‑1 (MQ=255) gggTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCaa > 1:936654/1‑123 (MQ=255) gggTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCaa < 2:936654/123‑1 (MQ=255) tataGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑c < 1:161818/93‑1 (MQ=255) tataGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑c > 2:161818/1‑93 (MQ=255) taGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑CCTCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGt > 2:1091815/1‑139 (MQ=255) gctgctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCa < 1:425295/139‑1 (MQ=255) aaaagaacacagcccaATAGTGACCACTTTTAATTTTCGGCCCAAAATTTCTCCCTTCAGTAAAGAAAACTGATATTTGGGCTATCCGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCa < 2:349409/126‑1 (MQ=255) cccGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGtata > 1:464412/1‑139 (MQ=255) gCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑cc‑ccccc < 1:230517/86‑1 (MQ=255) gCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑cc‑ccccc > 2:230517/1‑86 (MQ=255) ccaccaATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGa > 2:405920/1‑139 (MQ=255) caATAGTGACGACTTTTAATTTCTAGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTa < 1:1091815/139‑1 (MQ=255) tGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGAtt < 2:464412/139‑1 (MQ=255) acgacTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACTTAAAAAACCCAGCAACAGGACAAATATGGGAATTGTAAGGTATAACGAcctgttccttat > 1:349409/1‑128 (MQ=255) ttCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTc < 1:572189/83‑1 (MQ=255) ttCAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTc > 2:572189/1‑83 (MQ=255) cAGTACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTa < 1:405920/139‑1 (MQ=255) tACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTc < 2:156352/78‑1 (MQ=255) tACAGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTc > 1:156352/1‑78 (MQ=255) aGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGa > 1:958294/1‑58 (MQ=255) aGAATACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGa < 2:958294/58‑1 (MQ=255) aaTACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGa < 1:470367/116‑1 (MQ=255) aaTACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGa > 2:470367/1‑116 (MQ=255) tACTGATATCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTa > 2:612369/1‑139 (MQ=255) atatCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACa < 1:663174/46‑1 (MQ=255) atatCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACa > 2:663174/1‑46 (MQ=255) atatCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGa < 2:1020908/60‑1 (MQ=255) atatCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGa > 1:1020908/1‑60 (MQ=255) tCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGa < 2:380746/46‑1 (MQ=255) tCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGa > 1:380746/1‑46 (MQ=255) tCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGt < 1:968209/62‑1 (MQ=255) tCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGt > 2:968209/1‑62 (MQ=255) tCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCaa < 2:27007/96‑1 (MQ=255) tCTGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCaa > 1:27007/1‑96 (MQ=255) tGGCATATCTGCCCC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTaa > 2:372119/1‑139 (MQ=255) tGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGa > 2:842303/1‑44 (MQ=255) tGGCATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGa < 1:842303/44‑1 (MQ=255) cATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCtt < 2:1427/109‑1 (MQ=255) cATATCTGC‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCtt > 1:1427/1‑109 (MQ=255) | TCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑CC‑CCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTAA > NC_000913/1818412‑1818669 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |