| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,484,897 | (C)6→7 | coding (524/3594 nt) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,484,891 | 1 | . | C | 96.2% | 172.8 / ‑0.2 | 53 | coding (518/3594 nt) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (23/28); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.81e-01 | |||||||||||
CGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑C‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAG > NC_000913/2484756‑2485023 | cgccgcCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑c‑c > 1:821363/1‑139 (MQ=255) cgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑c‑cccc > 1:606917/1‑139 (MQ=255) ccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑c‑ccccca < 2:567746/139‑3 (MQ=255) tgctgcAACCAAACCACTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGTGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATtcatca > 1:977965/1‑139 (MQ=255) gctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCatcaa > 1:126382/1‑139 (MQ=255) caaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTcc < 2:263352/139‑1 (MQ=255) gATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaac > 1:34059/1‑139 (MQ=255) ccTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtct > 1:196894/1‑139 (MQ=255) ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAtt > 1:1015139/1‑132 (MQ=255) ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAtt < 2:1015139/132‑1 (MQ=255) tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCatcaatc > 1:777708/1‑112 (MQ=255) tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCatcaatc < 2:777708/112‑1 (MQ=255) tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCttt > 2:408348/1‑139 (MQ=255) tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCATACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑c‑cccccaga > 1:373478/1‑90 (MQ=255) tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCATACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑c‑cccccaga < 2:373478/94‑5 (MQ=255) cGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa < 2:126382/139‑1 (MQ=255) cGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa < 2:34059/139‑1 (MQ=255) cTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAt > 2:625853/1‑139 (MQ=255) cTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAt > 1:1085031/1‑139 (MQ=255) ccaccCTTCACGACTCAATGTGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAAGTAATTCATCAATCCTTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc < 2:977965/139‑1 (MQ=255) cccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAgg < 2:1085031/139‑1 (MQ=255) gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCa < 1:99263/94‑1 (MQ=255) gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCa > 2:99263/1‑94 (MQ=255) gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat > 2:253770/1‑127 (MQ=255) gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat < 1:253770/127‑1 (MQ=255) gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa > 1:707179/1‑139 (MQ=255) tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa > 2:291399/1‑136 (MQ=255) tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa < 1:291399/136‑1 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTaa < 2:2919/70‑1 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTaa > 1:2919/1‑70 (MQ=255) cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc < 1:454685/113‑1 (MQ=255) cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc > 2:454685/1‑113 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGca < 2:1038919/133‑1 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa < 2:223241/139‑1 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑c‑ccccca > 1:817272/1‑42 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑c‑ccccca < 2:817272/44‑3 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CTC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGca > 1:1038919/1‑133 (MQ=255) ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt < 1:408348/139‑1 (MQ=255) cAGTAAATATTTCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGACACAATGATTACTTTa < 1:840311/139‑1 (MQ=255) gTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa < 2:703674/68‑1 (MQ=255) gTAAATATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAtt < 1:625853/139‑1 (MQ=255) gTAAATATTGCTCGGGTAGCAAATTA‑C‑CGCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa > 1:703674/1‑68 (MQ=255) aaaTATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTAc > 2:184444/1‑131 (MQ=255) aaaTATTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTAc < 1:184444/131‑1 (MQ=255) atTGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagt < 2:821363/139‑1 (MQ=255) ttGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta < 2:707179/139‑1 (MQ=255) tGCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTaa > 2:1090604/1‑139 (MQ=255) gCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat < 2:234369/85‑1 (MQ=255) gCTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat > 1:234369/1‑85 (MQ=255) cTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcac > 1:625471/1‑114 (MQ=255) cTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcac < 2:625471/114‑1 (MQ=255) cTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca < 2:606917/139‑1 (MQ=255) cTCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca > 1:97113/1‑139 (MQ=255) tCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTtata < 1:1062871/82‑1 (MQ=255) tCGGGTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTtata > 2:1062871/1‑82 (MQ=255) gTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt < 1:815086/115‑1 (MQ=255) gTAGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt > 2:815086/1‑115 (MQ=255) aGCAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAc < 2:256881/139‑1 (MQ=255) cAAATTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACca > 1:340930/1‑139 (MQ=255) aaaTTACC‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACcag < 2:340930/139‑1 (MQ=255) | CGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑C‑C‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAG > NC_000913/2484756‑2485023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |