| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,970,032 | (G)6→7 | coding (1002/1047 nt) | wzzE → | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,970,026 | 1 | . | G | 97.1% | 120.3 / ‑3.1 | 35 | coding (996/1047 nt) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (17/17); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01 | |||||||||||
GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACG > NC_000913/3969888‑3970159 | ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTc > 1:376119/1‑139 (MQ=255) ttAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑ggggg‑gg < 2:247528/139‑2 (MQ=255) ttAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑ggggg‑gg < 1:601821/139‑2 (MQ=255) tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCgggg < 1:379252/139‑1 (MQ=255) ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTc > 2:311350/1‑139 (MQ=255) cAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCAt < 1:672270/139‑1 (MQ=255) cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGTCATTGTCGGGGGG‑GCTGATCGGGGc < 1:646646/114‑1 (MQ=255) cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGc > 2:646646/1‑114 (MQ=255) tATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa < 1:311350/139‑1 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACccgc < 1:261495/119‑1 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACccgc > 2:261495/1‑119 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca > 1:50505/1‑139 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca < 2:376119/139‑1 (MQ=255) cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTg > 1:550649/1‑92 (MQ=255) cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTg < 2:550649/92‑1 (MQ=255) cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACccgccg > 1:1052938/1‑111 (MQ=255) cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGt > 1:564446/1‑139 (MQ=255) cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCGGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACccgccg < 2:1052938/111‑1 (MQ=255) cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCAt < 1:720165/97‑1 (MQ=255) cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCAt > 2:720165/1‑97 (MQ=255) cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa < 2:523168/115‑1 (MQ=255) cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa > 1:523168/1‑115 (MQ=255) cGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCa < 1:55420/139‑1 (MQ=255) tGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACccgccg < 2:413601/70‑1 (MQ=255) tGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACccgccg < 2:835136/70‑1 (MQ=255) tGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACccgccg > 1:835136/1‑70 (MQ=255) tGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACccgccg > 1:413601/1‑70 (MQ=255) tGCCTTCCTGATGATTATGCGGGGCATTGTC‑GGGGGAGCTGATCGGGACTGGTGTCGCATTAAGCCGCCGTTGCTCGAAATAACAACGCTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg > 1:722650/1‑139 (MQ=255) ccTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc > 2:270852/1‑74 (MQ=255) ccTTCCTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 1:270852/74‑1 (MQ=255) ccTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACccgccg < 1:692804/64‑1 (MQ=255) ccTGATGATTATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACccgccg > 2:692804/1‑64 (MQ=255) tATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg > 2:1024154/1‑81 (MQ=255) tATGCGGGGCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg < 1:1024154/81‑1 (MQ=255) tgcggggCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTa > 2:1033210/4‑139 (MQ=255) ggCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTgg > 1:833459/1‑139 (MQ=255) gCATTGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGt > 1:263857/1‑139 (MQ=255) ttGTCGGGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAcg < 2:263857/139‑1 (MQ=255) | GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGG‑GCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACG > NC_000913/3969888‑3970159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |