| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,980,957 | (G)6→7 | coding (71/1386 nt) | yifK → | putative APC family amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,980,951 | 1 | . | G | 96.9% | 223.1 / ‑1.1 | 65 | coding (65/1386 nt) | yifK | putative APC family amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (30/33); total (31/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCG > NC_000913/3980821‑3981079 | acacTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑gggggggc < 1:1112073/139‑3 (MQ=255) acTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑gggggggcac > 2:777725/1‑135 (MQ=255) tCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCa > 2:971165/1‑139 (MQ=255) tGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCAG‑GGGGGGCACCATTgg > 2:831681/1‑139 (MQ=255) aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGt > 1:878314/1‑139 (MQ=255) cATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCgg > 2:168555/1‑139 (MQ=255) cATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCgg < 1:799008/139‑1 (MQ=255) aTAACGTTAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGc < 1:122602/139‑1 (MQ=255) aTAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGc < 1:971165/139‑1 (MQ=255) aCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCg < 1:313256/134‑1 (MQ=255) aCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCg > 2:313256/1‑134 (MQ=255) tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTa > 1:253202/1‑139 (MQ=255) tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTa < 2:792241/139‑1 (MQ=255) aCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATg < 1:831681/139‑1 (MQ=255) tAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc < 1:691536/139‑1 (MQ=255) tAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc > 2:809442/1‑139 (MQ=255) cacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc < 1:807168/139‑1 (MQ=255) tGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa < 1:336864/139‑1 (MQ=255) gCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAACGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaaa > 2:876075/1‑139 (MQ=255) acacaacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATggg < 1:965487/139‑1 (MQ=255) acaacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggcc > 2:770012/1‑139 (MQ=255) acacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccggg < 2:114720/139‑1 (MQ=255) cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc < 2:33424/139‑1 (MQ=255) cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc > 1:33424/1‑139 (MQ=255) gAGGATTTATGGCAGATAACAAACCGGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCAt < 2:588394/139‑1 (MQ=255) gAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCAt < 2:253202/139‑1 (MQ=255) aGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg > 1:126193/1‑107 (MQ=255) aGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg < 2:126193/107‑1 (MQ=255) ggATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATcc > 2:424239/1‑139 (MQ=255) gATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg < 1:809442/139‑1 (MQ=255) tttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg > 1:648039/1‑137 (MQ=255) tttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg < 2:648039/137‑1 (MQ=255) tATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAtt > 1:1059151/1‑139 (MQ=255) aTGGCAGATAACAAGCCAGGGCTACAGCGTGGGCTGGAAGCTCGACTTATCGAACTCATCGCCCGT‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGTGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg < 2:878314/139‑1 (MQ=255) aTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg < 1:777725/139‑1 (MQ=255) tGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt < 1:424239/139‑1 (MQ=255) aGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGc > 1:170031/1‑139 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGc > 2:489720/1‑82 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGc < 1:489720/82‑1 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGc < 1:142566/82‑1 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGc > 2:142566/1‑82 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc > 2:962352/1‑139 (MQ=255) cAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTatat < 1:168555/139‑1 (MQ=255) aCCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgttg < 1:100719/128‑1 (MQ=255) aCCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgttg > 2:100719/1‑128 (MQ=255) agagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg > 1:1005923/1‑122 (MQ=255) agagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg < 2:1005923/122‑1 (MQ=255) gagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCT‑T‑ggg < 2:659763/50‑1 (MQ=255) gagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCg > 2:87224/1‑65 (MQ=255) gagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCg < 1:87224/65‑1 (MQ=255) gagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑ggg > 1:659763/1‑50 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATgg < 2:969447/79‑1 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATgg > 1:969447/1‑79 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgttg > 1:830708/1‑123 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgttg < 2:830708/123‑1 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCg > 1:49740/1‑139 (MQ=255) aGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaaa > 2:725656/1‑93 (MQ=255) aGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaaa < 1:725656/93‑1 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc < 2:1042425/116‑1 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc > 1:1042425/1‑116 (MQ=255) cGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg > 1:910824/1‑58 (MQ=255) cGACATATCGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg < 2:910824/58‑1 (MQ=255) cGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCgg < 2:147154/37‑1 (MQ=255) cGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCgg > 1:147154/1‑37 (MQ=255) cGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAAt > 2:71598/1‑139 (MQ=255) cGAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAAt < 2:1059151/139‑1 (MQ=255) gAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTatat < 1:285/99‑1 (MQ=255) gAACTCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTatat > 2:285/1‑99 (MQ=255) tCATCGCCC‑TGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCg > 1:73822/1‑139 (MQ=255) | ACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCG > NC_000913/3980821‑3981079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |