Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	542,530	A→G	T215A (ACG→GCG)	glxK →	glycerate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	542,530	0	A	G	100.0%	198.4 / NA	55	T215A (ACG→GCG)	glxK	glycerate kinase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/28);  total (27/28)

GAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGG  >  NC_000913/542410‑542639                                                                                                                          |                                                                                                              gagaTGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCgaag                                                                                             >  2:584480/1‑139 (MQ=255) gagaTGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCgaag                                                                                             >  2:1125240/1‑139 (MQ=255)       gatgAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTg                                                                                       >  1:108371/1‑139 (MQ=255)             tGCGATCCGCGTCTGGCGAATTGCCATATTGCAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAgg                                                                                 >  2:844137/1‑139 (MQ=255)                 aTCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCt                                                                             >  1:134806/1‑139 (MQ=255)                 aTCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCt                                                                             >  2:1120925/1‑139 (MQ=255)                   ccGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGc                                                                           <  1:844137/139‑1 (MQ=255)                   ccGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGc                                                                           <  1:584480/139‑1 (MQ=255)                    cgcgTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCa                                                                          <  1:1125240/139‑1 (MQ=255)                        tCTGGCGAATTGCCATATTGAAGTAGCATGTGATGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAAt                                                                      <  1:529499/139‑1 (MQ=255)                            gCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACg                                                                  >  2:324583/1‑139 (MQ=255)                               aaTTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGccc                                                               <  2:1095827/139‑1 (MQ=255)                                aTTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                              <  2:108371/139‑1 (MQ=255)                                aTTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                              <  2:374493/139‑1 (MQ=255)                                aTTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                              <  2:134806/139‑1 (MQ=255)                                              gTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAg                                                 >  1:882825/1‑139 (MQ=255)                                              gTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGgaga                                                                                                      <  1:1005122/85‑1 (MQ=255)                                              gTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGgaga                                                                                                      >  2:1005122/1‑85 (MQ=255)                                                  cATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGc                                                                           >  1:1056023/1‑108 (MQ=255)                                                  cATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGc                                                                           <  2:1056023/108‑1 (MQ=255)                                                  cATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                            <  2:540181/139‑1 (MQ=255)                                                   aTGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCa                                               >  2:1046939/1‑135 (MQ=255)                                                   aTGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCa                                               <  1:1046939/135‑1 (MQ=255)                                                   aTGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACt                                           >  1:845504/1‑139 (MQ=255)                                                    tgtgACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTacaaca                                                  >  2:962401/1‑131 (MQ=255)                                                    tgtgACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTacaaca                                                  <  1:962401/131‑1 (MQ=255)                                                    tgtgACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCAGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGccc                                                               <  1:275047/118‑1 (MQ=255)                                                    tgtgACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCAGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGccc                                                               >  2:275047/1‑118 (MQ=255)                                                      tgACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGgaga                                                                                                      >  2:647443/1‑77 (MQ=255)                                                      tgACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGgaga                                                                                                      <  1:647443/77‑1 (MQ=255)                                                           ttGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGgaga                                                                                                      <  2:545092/72‑1 (MQ=255)                                                           ttGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGgaga                                                                                                      >  1:545092/1‑72 (MQ=255)                                                                     gCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGga                                                                                                        >  2:517260/1‑60 (MQ=255)                                                                     gCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGga                                                                                                        <  1:517260/60‑1 (MQ=255)                                                                      cTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                              <  1:628975/101‑1 (MQ=255)                                                                      cTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                              >  2:628975/1‑101 (MQ=255)                                                                        ggTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTa                                                       >  1:85157/1‑106 (MQ=255)                                                                        ggTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTa                                                       <  2:85157/106‑1 (MQ=255)                                                                        ggTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATg                      >  2:893655/1‑139 (MQ=255)                                                                        ggTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATg                      <  2:845504/139‑1 (MQ=255)                                                                             ggg‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcggg                 >  1:673066/1‑139 (MQ=255)                                                                                    gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAgg                                                                                 <  1:659172/69‑1 (MQ=255)                                                                                    gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                              <  1:638107/88‑1 (MQ=255)                                                                                    gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                              >  2:638107/1‑88 (MQ=255)                                                                                    gccgcgcggcgGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAgg                                                                                 >  2:659172/4‑69 (MQ=255)                                                                                        cgcggcgGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAAt                                  <  1:607621/112‑1 (MQ=255)                                                                                        cgcggcgGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAAt                                  >  2:607621/1‑112 (MQ=255)                                                                                          cggcggcggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                              <  1:301603/82‑1 (MQ=255)                                                                                          cggcggcggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                              >  2:301603/1‑82 (MQ=255)                                                                                            gcggcggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgg   <  1:324583/139‑1 (MQ=255)                                                                                             cggcggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg  <  2:882825/139‑1 (MQ=255)                                                                                              ggcggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCaa                                              >  1:423438/1‑94 (MQ=255)                                                                                              ggcggTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCaa                                              <  2:423438/94‑1 (MQ=255)                                                                                                             gCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                              <  2:864265/63‑1 (MQ=255)                                                                                                             gCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                              >  1:864265/1‑63 (MQ=255)                                                                                                                          |                                                                                                              GAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGG‑CACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGG  >  NC_000913/542410‑542639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑