Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,330,396	G→A	*478* (TAG→TAA)	dacB →	D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,330,396	0	G	A	100.0%	57.6 / NA	19	*478* (TAG→TAA)	dacB	D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/11);  total (8/11)

CAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑T‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGC  >  NC_000913/3330271‑3330503                                                                                                                                |                                                                                                            cAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAc                                                                                                 >  2:540495/1‑139 (MQ=255)   aCGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAccc                                                                                               <  1:463457/139‑1 (MQ=255)      aaTGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCgg                                                                                            <  1:404669/139‑1 (MQ=255)               tgttCAATAGCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGACGTATTACGTTAGTGCT‑TGTTTGAAAGCCGTCTGTATCAAGATATTTATCAGAACAATTAACCAGAAAGTTTCCCGGGAACATGGcc                                                                                   <  2:130455/139‑1 (MQ=255)                                    tGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACaa                                                              >  1:754345/1‑139 (MQ=255)                                            aaCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCGCATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt                                                      >  1:713532/1‑139 (MQ=255)                                             aCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCtt                                                     <  1:540495/139‑1 (MQ=255)                                                 gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGtt                                                 >  2:799621/1‑139 (MQ=255)                                                            tAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAcccc                                                                                              <  1:353477/83‑1 (MQ=255)                                                            tAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAcccc                                                                                              >  2:353477/1‑83 (MQ=255)                                                                                 gcgtt‑tttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTc                                                                           <  2:971890/77‑1 (MQ=255)                                                                                 gcgtt‑tttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTc                                                                           >  1:971890/5‑81 (MQ=255)                                                                                  cgtt‑tttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc                <  1:799621/136‑1 (MQ=255)                                                                                                 gTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc  <  2:562534/139‑1 (MQ=255)                                                                                                 gTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc  <  2:754345/139‑1 (MQ=255)                                                                                                  tttGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa                              <  2:1090462/110‑1 (MQ=255)                                                                                                  tttGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa                              >  1:1090462/1‑110 (MQ=255)                                                                                                                tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt                                                      <  2:389994/72‑1 (MQ=255)                                                                                                                tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt                                                      >  1:389994/1‑72 (MQ=255)                                                                                                                                |                                                                                                            CAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑T‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGC  >  NC_000913/3330271‑3330503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑