Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,029,729	(C)6→5	coding (191/921 nt)	yedA →	amino acid exporter for phenylalanine, threonine

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,029,724	0	C	.	100.0%	232.0 / NA	57	coding (186/921 nt)	yedA	amino acid exporter for phenylalanine, threonine
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (28/29);  total (28/29)

GGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGCC  >  NC_000913/2029597‑2029858                                                                                                                                |                                                                                                                                       ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGt                                                                                                                            >  1:1199634/1‑139 (MQ=255) ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGt                                                                                                                            >  1:1645608/1‑139 (MQ=255) ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGt                                                                                                                            >  2:334621/1‑139 (MQ=255) ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTCCTCTTGCGCGGCCAAAACCTccccccctccgt                                                                                                                             >  1:495393/1‑133 (MQ=255)   ggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTcc                                                                                                                          >  1:1222863/1‑139 (MQ=255)       cAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCgctg                                                                                                                      >  2:219043/1‑139 (MQ=255)        aaCCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCgctgc                                                                                                                     >  2:403906/1‑139 (MQ=255)           cTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCa                                                                                                                  <  1:403906/139‑1 (MQ=255)               tttGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGc                                                                                                              >  1:1505833/1‑139 (MQ=255)                    cATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCgctgct                                                                                                                    >  2:1119544/1‑128 (MQ=255)                    cATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCgctgct                                                                                                                    <  1:1119544/128‑1 (MQ=255)                    cATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCgcgc                                                                                                         >  1:1251029/1‑139 (MQ=255)                     aTTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCg                                                                                                                         <  1:201260/122‑1 (MQ=255)                     aTTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCg                                                                                                                         >  2:201260/1‑122 (MQ=255)                       tCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGa                                                                                                      <  2:1251029/139‑1 (MQ=255)                          gATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGATACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTg                                                                                                   <  2:1199634/139‑1 (MQ=255)                           aTTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTgg                                                                                                  <  2:1222863/139‑1 (MQ=255)                           aTTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTgg                                                                                                  <  1:334621/139‑1 (MQ=255)                                    gAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTg                                                                                              <  1:1276963/134‑1 (MQ=255)                                    gAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTg                                                                                              >  2:1276963/1‑134 (MQ=255)                                       aGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgct                                                                                      <  1:782198/139‑1 (MQ=255)                                                       tgatgGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGc                                                                      <  1:219043/139‑1 (MQ=255)                                                       tgatgGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGc                                                                      <  2:864857/139‑1 (MQ=255)                                                           ggcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATgg                                                                  >  2:245295/1‑139 (MQ=255)                                                             cgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGt                                                                                <  1:516416/123‑1 (MQ=255)                                                             cgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGt                                                                                >  2:516416/1‑123 (MQ=255)                                                                                  ccGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctggctg                                                                                 >  1:1233757/1‑101 (MQ=255)                                                                                  ccGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctggctg                                                                                 <  2:1233757/101‑1 (MQ=255)                                                                                           tATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCt                                                                                               <  2:124364/78‑1 (MQ=255)                                                                                           tATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCt                                                                                               >  1:124364/1‑78 (MQ=255)                                                                                                cTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGc                                                                      <  2:93914/98‑1 (MQ=255)                                                                                                cTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGc                                                                      >  1:93914/1‑98 (MQ=255)                                                                                                cTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTACTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCAt                             >  1:323840/1‑139 (MQ=255)                                                                                                  ggCATTTTTGCTACTGCGTGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcg                           >  2:1656033/1‑139 (MQ=255)                                                                                                        tttGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGccgccg                     <  1:1656033/139‑1 (MQ=255)                                                                                                        tttGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGccgccg                     <  2:1505833/139‑1 (MQ=255)                                                                                                                tGCGCGGACACAAACT‑CCCCCGCTACGTCCgctgct                                                                                                                    <  2:29288/36‑1 (MQ=255)                                                                                                                tGCGCGGACACAAACT‑CCCCCGCTACGTCCgctgct                                                                                                                    >  1:29288/1‑36 (MQ=255)                                                                                                                 gcgcGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctgg                                                                                    <  1:1516290/67‑1 (MQ=255)                                                                                                                 gcgcGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctgg                                                                                    >  2:1516290/1‑67 (MQ=255)                                                                                                                  cgcgGCCACAAACT‑CCCCCGCTACGTCCGCTGCTCAATg                                                                                                               <  1:1153684/39‑1 (MQ=255)                                                                                                                  cgcgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATg                                                                                                               >  2:1153684/1‑39 (MQ=255)                                                                                                                  cgcgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGc                                                                                                              >  2:755335/1‑40 (MQ=255)                                                                                                                  cgcgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGc                                                                                                              <  1:755335/40‑1 (MQ=255)                                                                                                                  cgcgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcg                           >  2:1313192/1‑123 (MQ=255)                                                                                                                  cgcgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcg                           <  1:1313192/123‑1 (MQ=255)                                                                                                                  cgcgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCgccgcc                      >  2:1106056/1‑128 (MQ=255)                                                                                                                  cgcgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCgccgcc                      <  1:1106056/128‑1 (MQ=255)                                                                                                                    cgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGc                                                                                                 >  2:227353/1‑51 (MQ=255)                                                                                                                    cgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGc                                                                                                 <  1:227353/51‑1 (MQ=255)                                                                                                                    cgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGc                                                                                                 >  1:1449006/1‑51 (MQ=255)                                                                                                                    cgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGc                                                                                                 <  2:1449006/51‑1 (MQ=255)                                                                                                                    cgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGc                                                                                                 <  2:885385/51‑1 (MQ=255)                                                                                                                    cgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGc                                                                                                 >  1:885385/1‑51 (MQ=255)                                                                                                                      gACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGt                                                                                <  1:1665383/66‑1 (MQ=255)                                                                                                                      gACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGt                                                                                >  2:1665383/1‑66 (MQ=255)                                                                                                                         acaAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTg    <  2:323840/139‑1 (MQ=255)                                                                                                                           aaaCT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:245295/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                       GGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGCC  >  NC_000913/2029597‑2029858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑