Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,214,339	(C)6→5	coding (306/1686 nt)	yehU ←	inner membrane putative sensory kinase in two‑component system with YehT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,214,334	0	C	.	100.0%	149.0 / NA	37	coding (311/1686 nt)	yehU	inner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (20/17);  total (20/17)

ACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAC  >  NC_000913/2214203‑2214468                                                                                                                                    |                                                                                                                                       aCGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccatc                                                                                                                                 <  2:483353/139‑4 (MQ=255)      aCGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAata                                                                                                                           <  2:723189/139‑1 (MQ=255)          cACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                      >  2:406492/1‑125 (MQ=255)          cACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                      <  1:406492/125‑1 (MQ=255)          cACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGa                                                                                                                       >  1:1121201/1‑139 (MQ=255)           aCCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGa                                                                                                                              >  2:819534/1‑131 (MQ=255)           aCCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGa                                                                                                                              <  1:819534/131‑1 (MQ=255)                   tAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAgccg                                                                                                              >  2:56907/1‑139 (MQ=255)                             aaaGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGcc                                                                                                    >  1:132421/1‑139 (MQ=255)                                 aCTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                                <  2:132421/139‑1 (MQ=255)                                    ttATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAg                                                                                             >  2:610009/1‑139 (MQ=255)                                           tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGa                                                                                                                              <  1:1411645/99‑1 (MQ=255)                                           tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGa                                                                                                                              >  2:1411645/1‑99 (MQ=255)                                                  gcggcgGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccg                                                                               <  1:633147/139‑1 (MQ=255)                                                     gcggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggac                                                                            >  1:880147/1‑139 (MQ=255)                                                                tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  1:56907/139‑1 (MQ=255)                                                                   tgtgTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                      <  1:726833/68‑1 (MQ=255)                                                                   tgtgTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                      >  2:726833/1‑68 (MQ=255)                                                                     tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccatc                                                                                                                                 <  2:626813/71‑4 (MQ=255)                                                                     tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccatc                                                                                                                                 >  1:626813/1‑68 (MQ=255)                                                                     tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGc                                                                                                                                        <  1:1590956/64‑1 (MQ=255)                                                                     tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGc                                                                                                                                        >  2:1590956/1‑64 (MQ=255)                                                                     tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                                                            >  1:47127/1‑139 (MQ=255)                                                                          cAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                      >  1:1525857/1‑61 (MQ=255)                                                                          cAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                      <  2:1525857/61‑1 (MQ=255)                                                                             gCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                                    <  2:880147/139‑1 (MQ=255)                                                                                        aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:47127/139‑1 (MQ=255)                                                                                        aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:1121201/139‑1 (MQ=255)                                                                                                aCGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                     >  2:1316033/1‑71 (MQ=255)                                                                                                aCGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                     <  1:1316033/71‑1 (MQ=255)                                                                                                    tGGTCGAGATCATGCAACTTAACGCGGTCATGcccccatcg                                                                                                                                >  2:475496/1‑37 (MQ=255)                                                                                                    tGGTCGAGATCATGCAACTTAACGCGGTCATGcccccatcg                                                                                                                                <  1:475496/41‑5 (MQ=255)                                                                                                    tGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                      <  1:179865/35‑1 (MQ=255)                                                                                                    tGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                      >  2:179865/1‑35 (MQ=255)                                                                                                    tGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  1:1276166/103‑1 (MQ=255)                                                                                                    tGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 >  2:1276166/1‑103 (MQ=255)                                                                                                      gTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAAt                           >  1:1331640/1‑139 (MQ=255)                                                                                                       tCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATTGAATATCGATGTAAGCCGCCgg                                                                                                          <  1:658857/59‑1 (MQ=255)                                                                                                       tCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                          >  2:658857/1‑59 (MQ=255)                                                                                                           gaTCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggacc                                                                           >  2:1372785/1‑86 (MQ=255)                                                                                                           gaTCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggacc                                                                           <  1:1372785/86‑1 (MQ=255)                                                                                                               aTGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTc                  >  2:1487962/1‑139 (MQ=255)                                                                                                                tGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 >  1:1283515/1‑139 (MQ=255)                                                                                                                tGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 >  1:1679874/1‑139 (MQ=255)                                                                                                                     cTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg            <  2:1331640/139‑1 (MQ=255)                                                                                                                          cgcgGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                     >  2:1657046/1‑45 (MQ=255)                                                                                                                          cgcgGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                     <  1:1657046/45‑1 (MQ=255)                                                                                                                               tCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  2:712708/76‑1 (MQ=255)                                                                                                                               tCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 >  1:712708/1‑76 (MQ=255)                                                                                                                               tCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGt                                    <  1:832100/105‑1 (MQ=255)                                                                                                                               tCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGt                                    >  2:832100/1‑105 (MQ=255)                                                                                                                               tCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAc  >  1:1429042/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                       ACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAC  >  NC_000913/2214203‑2214468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑