| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,250,629 | (C)5→6 | coding (210/1071 nt) | dhaK ← | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,250,624 | 1 | . | C | 97.7% | 142.0 / ‑3.8 | 43 | coding (215/1071 nt) | dhaK | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (23/19); total (24/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
TCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCC > NC_000913/1250486‑1250749 | tCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAgg < 2:617653/139‑1 (MQ=255) gTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑ccc > 2:566575/1‑139 (MQ=255) cGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑ccc‑cc < 1:772055/139‑1 (MQ=255) aGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa < 1:566575/139‑1 (MQ=255) gAATATCGCCGGTGTAA‑TTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 1:303370/139‑1 (MQ=255) cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa > 2:133373/1‑132 (MQ=255) cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa < 1:133373/132‑1 (MQ=255) cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGatat > 2:426872/1‑139 (MQ=255) cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGatat < 1:426872/139‑1 (MQ=255) cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGatat > 2:448295/1‑139 (MQ=255) tgtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATccc < 2:1220989/122‑1 (MQ=255) tgtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATccc > 1:1220989/1‑122 (MQ=255) aTTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa < 2:1232658/122‑1 (MQ=255) aTTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa > 1:1232658/1‑122 (MQ=255) tttGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCa < 1:291513/138‑1 (MQ=255) tttGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCa > 2:291513/1‑138 (MQ=255) gTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCACGTGTCCGc > 2:1146733/1‑139 (MQ=255) tACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCgtgt < 1:1592319/134‑1 (MQ=255) tACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCgtgt > 2:1592319/1‑134 (MQ=255) cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgc > 2:144093/1‑139 (MQ=255) acCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTTCGCTgcc > 2:472840/1‑139 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCCTGTCCGCTgccgc > 2:1123453/1‑139 (MQ=255) ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCAccc > 1:13376/1‑139 (MQ=255) gccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa > 1:1453612/1‑92 (MQ=255) gccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa < 2:1453612/92‑1 (MQ=255) aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGAcag > 2:104739/1‑139 (MQ=255) aCTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAgg < 1:104739/139‑1 (MQ=255) cTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAggg < 1:448295/139‑1 (MQ=255) aTGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACt < 2:13376/139‑1 (MQ=255) gcgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACtttt > 1:1598902/1‑139 (MQ=255) gcATTCAAAGATTTTATCTGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTcc > 2:965678/1‑139 (MQ=255) aTTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTg < 1:1123453/139‑1 (MQ=255) ttCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc > 2:1302484/1‑73 (MQ=255) ttCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 1:1302484/73‑1 (MQ=255) gATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAgg < 2:1598902/139‑1 (MQ=255) tttATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACcaca < 2:76004/76‑1 (MQ=255) tttATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCACCGAAAGCATCCCCTGACCGATATAACcaca > 1:76004/1‑76 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt > 2:1600149/1‑139 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt > 2:163048/1‑139 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt > 2:745821/1‑139 (MQ=255) tCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCa > 1:44967/1‑139 (MQ=255) ggCGTCGGTGAGGTGAAAATCTCGCCCGGACAGG‑c > 1:932806/1‑35 (MQ=255) ggCGTCGGTGAGGTGAAAATCTCGCCCGGACAGG‑c < 2:932806/35‑1 (MQ=255) gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt > 1:224968/1‑74 (MQ=255) gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt < 2:224968/74‑1 (MQ=255) cGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCg > 1:1114953/1‑139 (MQ=255) gTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTTCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg < 1:472840/139‑1 (MQ=255) tCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATcc < 1:144093/139‑1 (MQ=255) | TCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCC > NC_000913/1250486‑1250749 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |