| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,399,985 | (C)7→8 | coding (252/516 nt) | ogt ← | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,399,978 | 1 | . | C | 97.1% | 115.8 / ‑3.4 | 35 | coding (259/516 nt) | ogt | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (15/19); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATG > NC_000913/1399841‑1400104 | gggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTGCAGACTTCGCGCTGAAATGGCGT‑c < 2:1147630/139‑1 (MQ=255) aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc > 1:1571456/1‑139 (MQ=255) aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc‑ccccg < 2:1571456/139‑3 (MQ=255) cGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc‑ccccgtag < 2:1668942/139‑6 (MQ=255) ttGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc > 1:610508/1‑128 (MQ=255) ttGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc < 2:610508/128‑1 (MQ=255) tGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGt > 1:1265565/1‑139 (MQ=255) gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGa < 2:1311042/139‑1 (MQ=255) gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGa < 1:145095/139‑1 (MQ=255) cACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCaa < 1:619672/139‑1 (MQ=255) cACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGCATCaa < 2:1234720/139‑1 (MQ=255) aCGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCaat < 2:1230686/139‑1 (MQ=255) cgcgccgcgcCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAg < 1:1451552/129‑1 (MQ=255) cgcgccgcgcCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAg > 2:1451552/1‑129 (MQ=255) cgcgccgcgcCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCaata < 2:412981/139‑1 (MQ=255) cgcgccgcgcCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCaata < 2:1265565/139‑1 (MQ=255) cgcgccgcgcCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCaata > 1:323901/1‑139 (MQ=255) gcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCaa < 2:1378403/131‑1 (MQ=255) gcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCaa > 1:1378403/1‑131 (MQ=255) ttGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt < 1:677841/139‑1 (MQ=255) ttGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc‑ccccgtag < 2:618598/84‑6 (MQ=255) ttGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc‑ccccgtag > 1:618598/1‑79 (MQ=255) ccTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc‑ccccgtag > 1:1107766/1‑62 (MQ=255) ccTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCCCTCCCGTAg < 2:1107766/67‑1 (MQ=255) cTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTgg < 2:323901/139‑1 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc < 1:295235/56‑1 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc > 2:295235/1‑56 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc‑ccccgtag > 2:156478/1‑58 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc‑ccccgtag < 1:156478/63‑6 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑c < 1:50911/52‑1 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑c > 2:50911/1‑52 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCaa > 2:998485/1‑67 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCaa < 1:998485/67‑1 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCa < 1:251832/96‑1 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCa > 2:251832/1‑96 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgc > 1:1037876/1‑139 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCGGGATTGGTGGCAGAAATgc > 2:280564/1‑139 (MQ=255) gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTg < 2:210018/102‑1 (MQ=255) gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTg > 1:210018/1‑102 (MQ=255) gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc > 2:20976/1‑38 (MQ=255) gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc < 1:20976/38‑1 (MQ=255) ttCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt < 2:1054242/93‑1 (MQ=255) ttCCAGACTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGACTACCGGCAAAATATTCACGAAGCtt > 1:1054242/1‑93 (MQ=255) aCTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCaa < 2:1553967/50‑1 (MQ=255) aCTTCGCGCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCaa > 1:1553967/1‑50 (MQ=255) cgcgCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt > 2:706528/1‑139 (MQ=255) cgcgCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt > 2:1584717/1‑139 (MQ=255) cgcgCTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATAGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt > 1:1022048/1‑139 (MQ=255) cgcTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAg > 1:185989/1‑36 (MQ=255) cgcTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAg < 2:185989/36‑1 (MQ=255) gcTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAAt < 1:280564/139‑1 (MQ=255) cTGAAATGGCGTCCCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATg < 2:1022048/139‑1 (MQ=255) | GGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCCC‑CCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATG > NC_000913/1399841‑1400104 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |