| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,950,559 | (C)5→6 | intergenic (‑37/‑273) | aspS ← / → yecD | aspartyl‑tRNA synthetase/isochorismatase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,950,554 | 1 | . | C | 97.8% | 154.4 / ‑3.4 | 45 | intergenic (‑32/‑278) | aspS/yecD | aspartyl‑tRNA synthetase/isochorismatase family protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (20/24); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
GAAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑CCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAC > NC_000913/1950418‑1950683 | gAAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑cc > 2:1667384/1‑139 (MQ=255) aTCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑cccc‑cc < 1:752567/139‑2 (MQ=255) tACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGGCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTgcg < 2:1547836/139‑1 (MQ=255) ccAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑cccc‑cctg < 1:239129/132‑4 (MQ=255) ccAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑cccc‑cctg > 2:239129/1‑129 (MQ=255) cAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAg > 2:921656/1‑139 (MQ=255) tCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGAc > 2:1058888/1‑139 (MQ=255) cACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGAcc < 2:1679836/139‑1 (MQ=255) gacgacgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAg > 2:901322/1‑139 (MQ=255) acgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGc < 2:849770/139‑1 (MQ=255) acgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGc < 1:1667384/139‑1 (MQ=255) tGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGt < 1:977711/139‑1 (MQ=255) tGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGt < 2:23865/139‑1 (MQ=255) gACCCAACCACACAGAGTCACCTACTCCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGtt > 2:351393/1‑139 (MQ=255) cccAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAg < 1:901322/139‑1 (MQ=255) cccAACCACACAGAGTCACCTACTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAg < 1:351393/139‑1 (MQ=255) caGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTca > 1:660556/1‑139 (MQ=255) caGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTca < 1:1058888/139‑1 (MQ=255) ccccACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGaaaaa > 2:680602/1‑139 (MQ=255) cccACGGGGGACAAACGGAGCTTTCCACAATATTCTGTACGCATGAGATATCCCTGTACTTAGCTGCCGGCGGATGCCCCC‑CTGCAGCGCAGGTGGCCAAGTCGCAGCGTAACCTGTATGTCACAACTGCATGaaacaa < 2:287599/139‑1 (MQ=255) aCGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 1:921656/139‑1 (MQ=255) cGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTa < 1:1211705/114‑1 (MQ=255) cGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑CCCCTCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTa > 2:1211705/1‑114 (MQ=255) aCGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATg < 1:956137/62‑1 (MQ=255) aCGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATg > 2:956137/1‑62 (MQ=255) cTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGaaa > 1:1046902/1‑139 (MQ=255) tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑cccc‑c > 2:972992/1‑48 (MQ=255) tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑cccc‑c < 1:972992/48‑1 (MQ=255) tGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc > 2:1251791/1‑107 (MQ=255) tGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 1:1251791/107‑1 (MQ=255) tACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAag > 2:1379746/1‑139 (MQ=255) aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc > 2:1230672/1‑100 (MQ=255) aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 1:1230672/100‑1 (MQ=255) aTGAGATATCCCTTAACTTAGCTGCCGCCGGAAGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCt > 2:1006678/1‑139 (MQ=255) tCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGc > 1:742869/1‑115 (MQ=255) tCCCTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGc < 2:742869/115‑1 (MQ=255) ccTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGc < 2:1365314/62‑1 (MQ=255) ccTTAACTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGc > 1:1365314/1‑62 (MQ=255) aaCTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGaa > 1:1090025/1‑139 (MQ=255) aCTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGt < 1:1111168/37‑1 (MQ=255) aCTTAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGt > 2:1111168/1‑37 (MQ=255) tAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGt < 1:459830/61‑1 (MQ=255) tAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGt > 2:459830/1‑61 (MQ=255) tAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGc < 1:551623/138‑1 (MQ=255) tAGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGc > 2:551623/1‑138 (MQ=255) aGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAg > 2:454289/1‑79 (MQ=255) aGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAg < 1:454289/79‑1 (MQ=255) aGCTGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCt > 1:689736/1‑139 (MQ=255) tGCCGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGt < 2:689736/139‑1 (MQ=255) ccGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc > 2:936813/1‑76 (MQ=255) ccGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 1:936813/76‑1 (MQ=255) ccGGCGGATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCATTa < 2:1090025/139‑1 (MQ=255) cggcggATGCCCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAc < 2:557498/139‑1 (MQ=255) | GAAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑CCCC‑CTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAC > NC_000913/1950418‑1950683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |