| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,511,746 | (A)8→9 | coding (599/906 nt) | yhfZ ← | putative DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,511,738 | 1 | . | A | 96.9% | 206.6 / ‑1.6 | 65 | coding (607/906 nt) | yhfZ | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (33/30); total (33/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑A‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCC > NC_000913/3511604‑3511868 | cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑a‑aaa > 1:951309/1‑139 (MQ=255)cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑a‑aaa > 1:972105/1‑139 (MQ=255) ccTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑a‑aaaaaaaa > 2:1445149/1‑138 (MQ=255) tCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGt < 2:951309/139‑1 (MQ=255) ccAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCa < 2:247925/139‑1 (MQ=255) ccAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCa < 1:185872/139‑1 (MQ=255) cATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGAt > 1:661312/1‑139 (MQ=255) gTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTc > 1:1319415/1‑139 (MQ=255) aGTTCGTCTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGa < 2:1375561/139‑1 (MQ=255) ttCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATc > 1:141537/1‑139 (MQ=255) tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATccgccg < 2:1319415/139‑1 (MQ=255) gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACgg < 1:1445149/139‑1 (MQ=255) accacGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑a‑aaaaaaaaca < 2:331493/106‑4 (MQ=255) accacGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑a‑aaaaaaaaca > 1:331493/1‑103 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCa > 1:1066389/1‑96 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCa < 2:1066389/96‑1 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAc > 1:232094/1‑133 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAc < 2:232094/133‑1 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑a‑aaaaaa > 2:420057/1‑85 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑a‑aaaaaa < 1:420057/85‑1 (MQ=255) cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACgg > 2:332303/1‑117 (MQ=255) cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACgg < 1:332303/117‑1 (MQ=255) cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 1:521544/1‑132 (MQ=255) cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 2:521544/132‑1 (MQ=255) cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa > 1:1512798/1‑139 (MQ=255) aCATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACgg > 2:1111272/1‑112 (MQ=255) aCATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACgg < 1:1111272/112‑1 (MQ=255) aTCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACg > 2:345952/1‑134 (MQ=255) aTCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACg < 1:345952/134‑1 (MQ=255) cTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt < 2:1512798/139‑1 (MQ=255) tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGAt < 1:877101/91‑1 (MQ=255) tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGAt > 2:877101/1‑91 (MQ=255) tGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATccgccg > 1:67333/1‑86 (MQ=255) tGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATccgccg < 2:67333/86‑1 (MQ=255) tGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTg > 2:917373/1‑119 (MQ=255) tGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTg < 1:917373/119‑1 (MQ=255) tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACgg > 2:289191/1‑84 (MQ=255) tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACgg < 1:289191/84‑1 (MQ=255) tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt < 1:1520308/118‑1 (MQ=255) tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt > 2:1520308/1‑118 (MQ=255) tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGc > 1:1156986/1‑139 (MQ=255) aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 2:275411/1‑92 (MQ=255) aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 1:275411/92‑1 (MQ=255) aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc < 2:661312/139‑1 (MQ=255) agagaTCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCaa < 2:141537/139‑1 (MQ=255) agagaTCGACTCGTTCCACATCACTACCGCC‑a‑aaaaaaaaca < 1:23804/42‑4 (MQ=255) agagaTCGACTCGTTCCACATCACTACCGCC‑a‑aaaaaaaaca > 2:23804/1‑39 (MQ=255) agaTCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCATCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACg > 2:1070062/1‑139 (MQ=255) gaTCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGAt < 2:112465/58‑1 (MQ=255) gaTCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGAt > 1:112465/1‑58 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg < 1:393458/127‑1 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg > 2:393458/1‑127 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc > 2:559935/1‑130 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc < 1:559935/130‑1 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa > 2:432460/1‑139 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCACCCCCACGCGCTTCACGCTTGCGGACTCGTCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa > 2:1160903/1‑139 (MQ=255) gACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 2:822168/1‑80 (MQ=255) gACTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 1:822168/80‑1 (MQ=255) aCTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc > 2:1520453/1‑99 (MQ=255) aCTCGTTCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc < 1:1520453/99‑1 (MQ=255) cGTTCCCCATCACTACCGCC‑A‑AAAAAAACCATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGc < 2:972105/139‑1 (MQ=255) tCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc > 2:844942/1‑120 (MQ=255) tCCACATCACTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc < 1:844942/120‑1 (MQ=255) cTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt < 2:810229/69‑1 (MQ=255) cTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt > 1:810229/1‑69 (MQ=255) cTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTcc > 2:1364359/1‑139 (MQ=255) cTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTcc > 2:673292/1‑139 (MQ=255) cTACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTcc > 1:599871/1‑139 (MQ=255) tACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGaa > 2:289780/1‑45 (MQ=255) tACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGaa < 1:289780/45‑1 (MQ=255) tACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg > 2:361767/1‑125 (MQ=255) tACCGCCAA‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg < 1:361767/125‑1 (MQ=255) tACCGCCAA‑AAAAAAA‑CAGCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg > 1:1114373/1‑93 (MQ=255) tACCGCC‑AGAAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCTAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg < 2:1114373/93‑1 (MQ=255) | CGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑A‑AAAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCC > NC_000913/3511604‑3511868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |