| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,474,138 | (A)6→7 | coding (15/594 nt) | bdcR → | transcriptional repressor for divergent bdcA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,474,132 | 1 | . | A | 98.1% | 165.8 / ‑3.7 | 53 | coding (9/594 nt) | bdcR | transcriptional repressor for divergent bdcA |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (27/25); total (27/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01 | |||||||||||
ACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGG > NC_000913/4474009‑4474263 | aCTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑aa > 2:555359/1‑126 (MQ=255)aCTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑aa < 1:555359/126‑1 (MQ=255) tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGtt > 2:1265524/1‑139 (MQ=255) ccTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCt < 1:926939/139‑1 (MQ=255) cgcCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCg > 2:482064/1‑127 (MQ=255) cgcCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCg < 1:482064/127‑1 (MQ=255) cccATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg < 2:298405/133‑1 (MQ=255) cccATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg > 1:298405/1‑133 (MQ=255) cATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAt > 1:271725/1‑139 (MQ=255) ggACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTg < 2:271725/139‑1 (MQ=255) tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGttt > 2:258139/1‑112 (MQ=255) tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGttt < 1:258139/112‑1 (MQ=255) cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa > 2:1002839/1‑139 (MQ=255) tatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg < 2:1183991/110‑1 (MQ=255) tatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg > 1:1183991/1‑110 (MQ=255) aCATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc > 1:228/1‑99 (MQ=255) aCATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc < 2:228/99‑1 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTcg > 2:236350/1‑67 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTcg < 1:236350/67‑1 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAt > 1:1436001/1‑109 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAt < 2:1436001/109‑1 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa > 2:183684/1‑139 (MQ=255) aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg < 1:475088/98‑1 (MQ=255) aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg > 2:475088/1‑98 (MQ=255) aaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGa < 1:1265524/139‑1 (MQ=255) aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTcg > 1:1437315/1‑57 (MQ=255) aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTcg < 2:1437315/57‑1 (MQ=255) cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTg < 1:667882/87‑1 (MQ=255) cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTg > 2:667882/1‑87 (MQ=255) cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGc > 1:1673160/1‑139 (MQ=255) ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTcg < 2:936962/52‑1 (MQ=255) ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTcg > 1:936962/1‑52 (MQ=255) ttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAgg < 2:58823/59‑1 (MQ=255) ttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAgg > 1:58823/1‑59 (MQ=255) ttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGa < 2:1270145/69‑1 (MQ=255) ttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGa > 1:1270145/1‑69 (MQ=255) tAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAGTCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAg < 1:1313725/139‑1 (MQ=255) tAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAg < 1:1002839/139‑1 (MQ=255) aaCTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAgt < 1:183684/139‑1 (MQ=255) aaCAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCtttt < 1:328885/100‑1 (MQ=255) aaCAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCtttt > 2:328885/1‑100 (MQ=255) gTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGgt > 1:48769/1‑42 (MQ=255) gTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGgt < 2:48769/42‑1 (MQ=255) gggacgaTGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGc > 1:788556/7‑81 (MQ=255) gggacgaTGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGc < 2:788556/75‑1 (MQ=255) tAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATc < 2:1673160/139‑1 (MQ=255) ggTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc > 1:1436549/1‑51 (MQ=255) ggTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc < 2:1436549/51‑1 (MQ=255) ggTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg > 1:704373/1‑139 (MQ=255) ggTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg > 2:429973/1‑139 (MQ=255) ggTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg > 2:310784/1‑139 (MQ=255) ggTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg > 1:636211/1‑139 (MQ=255) gTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaaa < 1:591422/75‑1 (MQ=255) gTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaaa > 2:591422/1‑75 (MQ=255) aCT‑AAAAAATCAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTAGCTGAAGTTACTGATTctct < 1:1149504/132‑1 (MQ=255) | ACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGG > NC_000913/4474009‑4474263 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |