Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,447	T→C	E35G (GAG→GGG)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,447	0	T	C	100.0%	151.0 / NA	43	E35G (GAG→GGG)	araD	L‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/22);  total (21/22)

TTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTT  >  NC_000913/66338‑66577                                                                                                              |                                                                                                                                    tttCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCAt                                                                                                       >  1:38429/1‑139 (MQ=255)       aCCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGGTCAACGGCGCTGACGTTG‑CCCCATGTGAg                                                                                                  <  2:563246/138‑1 (MQ=255)       aCCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGGTCAACGGCGCTGACGTTG‑CCCCATGTGAg                                                                                                  >  1:563246/1‑138 (MQ=255)        ccTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCg                                                                                                <  2:3689/139‑1 (MQ=255)           tCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGa                                                                                             >  1:338938/1‑139 (MQ=255)               ccACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAg                                                                                         >  1:948282/1‑139 (MQ=255)                 aCTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGt                                                                                       <  1:532817/139‑1 (MQ=255)                 aCTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGt                                                                                       <  2:338938/139‑1 (MQ=255)                  cTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCCATGTGAGCGTGACCAGGt                                                                                       >  1:551571/1‑139 (MQ=255)                       ccGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGt                                                                                       <  2:592220/133‑1 (MQ=255)                       ccGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGt                                                                                       >  1:592220/1‑133 (MQ=255)                        cGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGttt                                                                                >  1:451051/1‑139 (MQ=255)                           tttCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTgg                                                                             <  2:451051/139‑1 (MQ=255)                             tCGATGCTAACAACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCa                                                                           >  2:96412/1‑139 (MQ=255)                                     aaCCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAgg                                                                   <  2:606785/139‑1 (MQ=255)                                          cGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGc                                                              <  2:901474/139‑1 (MQ=255)                                             ccATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTc                                                           >  2:792171/1‑139 (MQ=255)                                                tatCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGc                                                                            >  1:781879/1‑119 (MQ=255)                                                tatCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGc                                                                            <  2:781879/119‑1 (MQ=255)                                                tatCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTaa                                                        >  1:675553/1‑139 (MQ=255)                                                tatCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTaa                                                        >  2:570563/1‑139 (MQ=255)                                                tatCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTaa                                                        >  2:643679/1‑139 (MQ=255)                                                     tCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATAcc                                                    <  2:414625/138‑1 (MQ=255)                                                     tCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATAcc                                                    >  1:414625/1‑138 (MQ=255)                                                                gACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTgaga                                        <  2:134953/139‑1 (MQ=255)                                                                            gACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACg                                                                                                                 >  2:754345/1‑55 (MQ=255)                                                                            gACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACg                                                                                                                 <  1:754345/55‑1 (MQ=255)                                                                                 cGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAgg                                                                   >  1:1225401/1‑95 (MQ=255)                                                                                 cGGAAGGTTTGAGCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAgg                                                                   <  2:1225401/95‑1 (MQ=255)                                                                                  ggAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGAc                       <  2:551571/139‑1 (MQ=255)                                                                                         ttGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGt               <  1:570563/139‑1 (MQ=255)                                                                                                 aaaGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCt                                                          <  1:629299/88‑1 (MQ=255)                                                                                                 aaaGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCt                                                          >  2:629299/1‑88 (MQ=255)                                                                                                 aaaGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTgaga                                        >  2:1524650/1‑106 (MQ=255)                                                                                                 aaaGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTgaga                                        <  1:1524650/106‑1 (MQ=255)                                                                                                   aGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTgg                                                                             <  2:31759/67‑1 (MQ=255)                                                                                                   aGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTgg                                                                             >  1:31759/1‑67 (MQ=255)                                                                                                   aGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATgcg     <  2:38429/139‑1 (MQ=255)                                                                                                   aGACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATgcg     <  2:948282/139‑1 (MQ=255)                                                                                                    gACGCCGCGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATgcgc    <  1:792171/139‑1 (MQ=255)                                                                                                      cgccgcGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGtt                                                                 <  2:759938/76‑1 (MQ=255)                                                                                                      cgccgcGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGtt                                                                 >  1:759938/1‑76 (MQ=255)                                                                                                      cgccgcGCCCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCtt  >  2:1643325/1‑139 (MQ=255)                                                                                                              |                                                                                                                                    TTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTG‑CCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTT  >  NC_000913/66338‑66577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑