Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	167,743	C→T	T87I (ACC→ATC)	fhuA →	ferrichrome outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	167,743	0	C	T	100.0%	216.5 / NA	66	T87I (ACC→ATC)	fhuA	ferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/33);  total (33/33)

CCGTTACCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGA  >  NC_000913/167611‑167875                                                                                                                                      |                                                                                                                                     ccGTTACCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGa                                                                                                                                >  2:500139/1‑139 (MQ=255)        cGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATg                                                                                                                         >  2:1673280/1‑139 (MQ=255)        cGCTGCACCTGCGCCGC‑AGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATgg                                                                                                                        >  2:1178705/1‑139 (MQ=255)           tGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGgcg                                                                                                                      <  1:1400161/139‑1 (MQ=255)             cACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCt                                                                                                                    >  2:99807/1‑139 (MQ=255)             cACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCt                                                                                                                    >  2:384770/1‑139 (MQ=255)                  gcgcCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGTGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATc                                                                                                               >  2:353163/1‑139 (MQ=255)                   cgccgcAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGgcg                                                                                                                      <  2:1437647/131‑1 (MQ=255)                   cgccgcAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGgcg                                                                                                                      >  1:1437647/1‑131 (MQ=255)                        cAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGAtcgc                                                                                                                                   >  2:778121/1‑113 (MQ=255)                        cAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGAtcgc                                                                                                                                   <  1:778121/113‑1 (MQ=255)                         aaGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGaa                                                                                                        <  1:1408973/139‑1 (MQ=255)                              aGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCgg                                                                                                   <  2:187692/139‑1 (MQ=255)                                cgcATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGgcgc                                                                                                                     <  2:990475/119‑1 (MQ=255)                                cgcATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGgcgc                                                                                                                     >  1:990475/1‑119 (MQ=255)                                     gggggCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCCATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGCTGACGCTGCATCAGCCGAAGTCGGAAAaaga                                                                                            >  2:49084/1‑139 (MQ=255)                                       gggCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAaagaag                                                                                          <  2:1371975/139‑1 (MQ=255)                                              ctgcAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTa                                                                                    >  2:668995/1‑139 (MQ=255)                                              ctgcAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAag                                                                                                                              <  1:640570/96‑1 (MQ=255)                                              ctgcAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTATGATCGCCGAag                                                                                                                              >  2:640570/1‑96 (MQ=255)                                                       ttGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAgaga                                                                                                                           >  2:1422008/1‑90 (MQ=255)                                                       ttGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAgaga                                                                                                                           <  1:1422008/90‑1 (MQ=255)                                                        tGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCg                                                                         >  1:210982/1‑139 (MQ=255)                                                           ggcgCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAaagaa                                                                                           <  1:1448586/118‑1 (MQ=255)                                                           ggcgCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAaagaa                                                                                           >  2:1448586/1‑118 (MQ=255)                                                             cgcgACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAGAGAAGCGCTTAGCTACACGCCGGgtgt                                                                    <  1:598952/139‑1 (MQ=255)                                                             cgcgACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgtgt                                                                    <  1:500139/139‑1 (MQ=255)                                                             cgcgACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgtgt                                                                    <  1:1673280/139‑1 (MQ=255)                                                                gACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGtctc                                                                 <  1:384770/139‑1 (MQ=255)                                                                gACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGtctc                                                                 <  1:99807/139‑1 (MQ=255)                                                                 aCAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGgcg                                                                                                                      >  2:1023977/1‑85 (MQ=255)                                                                 aCAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGgcg                                                                                                                      <  1:1023977/85‑1 (MQ=255)                                                                        gCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGAt                                                                                                                          >  2:140376/1‑74 (MQ=255)                                                                        gCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGAt                                                                                                                          <  1:140376/74‑1 (MQ=255)                                                                          tACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCt                                                                                      >  1:1469998/1‑108 (MQ=255)                                                                          tACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCt                                                                                      <  2:1469998/108‑1 (MQ=255)                                                                          tACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTAcg                                                       >  2:685501/1‑139 (MQ=255)                                                                                cACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAgcg                                                                                        >  1:1411034/1‑100 (MQ=255)                                                                                cACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAgcg                                                                                        <  2:1411034/100‑1 (MQ=255)                                                                                     aaaCCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATc                                            <  2:210982/139‑1 (MQ=255)                                                                                           aTACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAgaga                                                                                                                           <  2:1192876/54‑1 (MQ=255)                                                                                           aTACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAgaga                                                                                                                           >  1:1192876/1‑54 (MQ=255)                                                                                           aTACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCg                                                                         >  1:1355438/1‑104 (MQ=255)                                                                                           aTACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCg                                                                         <  2:1355438/104‑1 (MQ=255)                                                                                           aTACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAacac                                      <  1:362550/139‑1 (MQ=255)                                                                                             aCGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCaa                                         <  2:1623702/134‑1 (MQ=255)                                                                                             aCGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCaa                                         >  1:1623702/1‑134 (MQ=255)                                                                                             aCGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCt                                    >  2:504537/1‑139 (MQ=255)                                                                                                 cGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCt                                                                                      <  1:563534/85‑1 (MQ=255)                                                                                                 cGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCt                                                                                      >  2:563534/1‑85 (MQ=255)                                                                                                   aTTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgtgt                                                                    >  2:1085373/1‑101 (MQ=255)                                                                                                   aTTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgtgt                                                                    <  1:1085373/101‑1 (MQ=255)                                                                                                                ccACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGgc                                                 <  2:1299955/108‑1 (MQ=255)                                                                                                                ccACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGgc                                                 >  1:1299955/1‑108 (MQ=255)                                                                                                                  acaGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTcgc                <  1:353163/139‑1 (MQ=255)                                                                                                                      tCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgtgt                                                                    <  2:1391178/83‑1 (MQ=255)                                                                                                                      tCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgtgt                                                                    >  1:1391178/1‑83 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGcc                                                                          <  1:231494/67‑1 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGcc                                                                          >  2:231494/1‑67 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgt                                                                      <  1:633309/71‑1 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgt                                                                      >  2:633309/1‑71 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATg                                 >  2:412108/1‑108 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATg                                 <  1:412108/108‑1 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGa  >  1:614411/1‑139 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGa  <  2:614411/139‑1 (MQ=255)                                                                                                                                ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGa  >  1:1519034/1‑139 (MQ=255)                                                                                                                                  gtgATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCa                                              >  2:112851/1‑93 (MQ=255)                                                                                                                                  gtgATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCa                                              <  1:112851/93‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     CCGTTACCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTC‑AAAAAGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGA  >  NC_000913/167611‑167875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑