| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,645,013 | C→T | *771* (TAG→TAA) | pbpC ← | penicillin‑insensitive murein repair transglycosylase, inactive transpeptidase domain protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,645,013 | 0 | C | T | 98.0% | 160.8 / ‑4.1 | 51 | coding (2313/2313 nt) | pbpC | penicillin‑insensitive murein repair transglycosylase, inactive transpeptidase domain protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (26/24); minor base . (1/0); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAACCAC > NC_000913/2644893‑2645148 | gtacagacaaGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTc > 1:908371/10‑139 (MQ=255) tACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGt > 2:1330120/1‑139 (MQ=255) cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGAt > 1:1134709/1‑139 (MQ=255) cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGAt > 2:957220/1‑135 (MQ=255) cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGAt < 1:957220/135‑1 (MQ=255) gagaTGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGcc < 2:1134709/139‑1 (MQ=255) ggCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACg > 2:247030/1‑139 (MQ=255) cgcgGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACAATTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCa < 2:1446555/117‑1 (MQ=255) cgcgGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACAATTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCa > 1:1446555/1‑117 (MQ=255) aGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGGGTTTTGGCAACAACAAGGAACAAAAAAGA‑TATTTCAATGACAAATTCAACTGTCGCGATTGGCCCAACGT‑ATCCAATAACCAGCa > 1:1626229/1‑139 (MQ=255) gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGt > 1:1331393/1‑123 (MQ=255) gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGt < 2:1331393/123‑1 (MQ=255) aaCAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGcc > 1:208198/1‑115 (MQ=255) aaCAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGcc < 2:208198/115‑1 (MQ=255) aaCAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAAtt > 2:1389407/1‑139 (MQ=255) cAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAAt > 1:367308/1‑136 (MQ=255) cAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAAt < 2:367308/136‑1 (MQ=255) gggCCGTTAACAATGAATGAA‑GTAACAATTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATaa < 2:1626229/139‑1 (MQ=255) ggCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGcc > 1:383383/1‑110 (MQ=255) ggCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGcc < 2:383383/110‑1 (MQ=255) ggCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATaa < 1:242554/139‑1 (MQ=255) ccGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATc > 1:182194/1‑139 (MQ=255) gACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑a < 1:518443/76‑1 (MQ=255) gACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑a > 2:518443/1‑76 (MQ=255) tATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGt < 2:805544/106‑1 (MQ=255) tATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGt > 1:805544/1‑106 (MQ=255) tATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTAt < 1:1389407/139‑1 (MQ=255) tATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTAt < 2:182194/139‑1 (MQ=255) atgaAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCACCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTc > 2:1681050/1‑139 (MQ=255) ggTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCaaa < 1:1640808/137‑1 (MQ=255) ggTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCaaa > 2:1640808/1‑137 (MQ=255) ggTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCAAATg < 1:1330120/139‑1 (MQ=255) ggTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCAAATg > 1:1292853/1‑139 (MQ=255) tAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCa < 1:247030/139‑1 (MQ=255) aaCATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAg > 1:846267/1‑139 (MQ=255) aaCATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAg > 2:151146/1‑139 (MQ=255) cATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTAt < 1:195558/124‑1 (MQ=255) cATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTAt > 2:195558/1‑124 (MQ=255) cATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATG‑‑AAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTc > 2:414918/1‑139 (MQ=255) aatGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGC‑ATG‑‑AAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGtt < 1:414918/139‑1 (MQ=255) aaTCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAAt > 2:1675925/1‑83 (MQ=255) aaTCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAAt < 1:1675925/83‑1 (MQ=255) aaTCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCg > 2:1052269/1‑139 (MQ=255) aaTCAGGGACAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCg < 2:1292853/139‑1 (MQ=255) aCAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTAt > 2:1188818/1‑84 (MQ=255) aCAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTAt < 1:1188818/84‑1 (MQ=255) cAAAACAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGa < 2:846267/139‑1 (MQ=255) aaaaCAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTc < 2:1570567/87‑1 (MQ=255) aaaaCAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTc > 1:1570567/1‑87 (MQ=255) aaCAGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAac > 1:621359/1‑139 (MQ=255) aGATTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAaccac < 2:908371/139‑1 (MQ=255) | GTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGC‑ATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCC‑ATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAACCAC > NC_000913/2644893‑2645148 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |