Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,271,932	T→C	T274A (ACG→GCG)	garR ←	tartronate semialdehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,271,932	0	T	C	100.0%	269.9 / NA	74	T274A (ACG→GCG)	garR	tartronate semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/40);  total (34/40)

CGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGC  >  NC_000913/3271796‑3272063                                                                                                                                         |                                                                                                                                     cGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTc                                                                                                                                    >  2:432856/1‑139 (MQ=255)  ggAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTcc                                                                                                                                   <  1:898670/139‑1 (MQ=255)                  aTTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGc                                                                                                                  <  1:1308551/139‑1 (MQ=255)                     cGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGt                                                                                                               >  1:828538/1‑139 (MQ=255)                     cGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGt                                                                                                               <  2:825831/139‑1 (MQ=255)                     cGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGt                                                                                                               >  1:42908/1‑139 (MQ=255)                      ggCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTg                                                                                                              <  1:731062/139‑1 (MQ=255)                        ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGcc                                                                                                            <  1:1362131/139‑1 (MQ=255)                            gATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGca                                                                                                        <  1:367071/139‑1 (MQ=255)                              ttCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGcatc                                                                                                      <  2:828538/139‑1 (MQ=255)                                 cAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCatcatc                                                                                                   >  1:1527852/1‑139 (MQ=255)                                         gATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAAc                                                                                           >  1:1675526/1‑139 (MQ=255)                                                   cGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGcc                                                                                                            >  1:419813/1‑112 (MQ=255)                                                   cGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGcc                                                                                                            <  2:419813/112‑1 (MQ=255)                                                   cGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCtgtg                                                                                 >  1:62419/1‑139 (MQ=255)                                                    gCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCAGGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGa                                                                                >  1:1005207/1‑139 (MQ=255)                                                      cATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGc                                                                                                                  <  2:1152546/103‑1 (MQ=255)                                                      cATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGc                                                                                                                  >  1:1152546/1‑103 (MQ=255)                                                      cATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGc                                                                              >  1:1649850/1‑139 (MQ=255)                                                        tCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCgg                                                                            >  2:809704/1‑139 (MQ=255)                                                                  acgTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTgcg                                                                  >  2:499963/1‑139 (MQ=255)                                                                   cgTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCa                                                                                                                 >  1:96485/1‑91 (MQ=255)                                                                   cgTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCa                                                                                                                 <  2:96485/91‑1 (MQ=255)                                                                       aTTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGAc                                                             <  2:62419/139‑1 (MQ=255)                                                                              aGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGc                                                                                                                  >  2:1272934/1‑79 (MQ=255)                                                                              aGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGc                                                                                                                  <  1:1272934/79‑1 (MQ=255)                                                                               gTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCAt                                                                                              <  2:1138416/98‑1 (MQ=255)                                                                               gTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCAt                                                                                              >  1:1138416/1‑98 (MQ=255)                                                                               gTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTgag                                                     >  1:1454987/1‑139 (MQ=255)                                                                                tAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTgaga                                                    >  2:725284/1‑139 (MQ=255)                                                                                tAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTgag                                                     <  1:139137/138‑1 (MQ=255)                                                                                tAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTgag                                                     >  2:139137/1‑138 (MQ=255)                                                                                 aaCTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGaa                                                   <  2:1454987/139‑1 (MQ=255)                                                                                 aaCTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGaa                                                   <  2:1649850/139‑1 (MQ=255)                                                                                  aCTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTtgtg                                                                    <  2:465090/121‑1 (MQ=255)                                                                                  aCTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTtgtg                                                                    >  1:465090/1‑121 (MQ=255)                                                                                  aCTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAg                                                  >  2:757415/1‑139 (MQ=255)                                                                                        aCTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCa                                            <  1:499963/139‑1 (MQ=255)                                                                                         cTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCa                                            >  1:376243/1‑139 (MQ=255)                                                                                         cTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGTGCCGACGCCGTGAGAAGTATCCAg                                           >  1:486172/1‑139 (MQ=255)                                                                                            tCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCt                                                                                    >  1:365483/1‑95 (MQ=255)                                                                                            tCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCt                                                                                    <  2:365483/95‑1 (MQ=255)                                                                                             cGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCa                                       <  1:725284/139‑1 (MQ=255)                                                                                                  gTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGc                                  <  2:1527852/139‑1 (MQ=255)                                                                                                   tttttCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGcc                                 <  2:1005207/139‑1 (MQ=255)                                                                                                      ttCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAg                               <  2:376243/139‑1 (MQ=255)                                                                                                       tCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGAt                             <  2:42908/139‑1 (MQ=255)                                                                                                          tagtagCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCt                          <  1:757415/139‑1 (MQ=255)                                                                                                           agtagCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGAc                                                             >  2:931095/1‑103 (MQ=255)                                                                                                           agtagCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGAc                                                             <  1:931095/103‑1 (MQ=255)                                                                                                            gtagCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTa                        <  1:432856/139‑1 (MQ=255)                                                                                                              agCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAat                      <  1:809704/139‑1 (MQ=255)                                                                                                                cACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCgg                                                                            >  2:812149/1‑83 (MQ=255)                                                                                                                cACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCgg                                                                            <  1:812149/83‑1 (MQ=255)                                                                                                                cACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGaa                                                   >  2:901208/1‑108 (MQ=255)                                                                                                                cACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGaa                                                   <  1:901208/108‑1 (MQ=255)                                                                                                                cACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAatat                    >  2:649127/1‑139 (MQ=255)                                                                                                                 aCGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATg                   <  2:1675526/139‑1 (MQ=255)                                                                                                                 aCGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATg                   <  1:829010/139‑1 (MQ=255)                                                                                                                  cGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGt                                                                                                               >  1:953744/1‑46 (MQ=255)                                                                                                                  cGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGt                                                                                                               <  2:953744/46‑1 (MQ=255)                                                                                                                  cGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGca                                                                                                        <  2:383024/53‑1 (MQ=255)                                                                                                                  cGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGca                                                                                                        >  1:383024/1‑53 (MQ=255)                                                                                                                    ccAGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGTGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAg                <  2:486172/139‑1 (MQ=255)                                                                                                                      aGGGCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGAt              <  2:608884/139‑1 (MQ=255)                                                                                                                       gggCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGcc                                 >  2:470577/1‑119 (MQ=255)                                                                                                                       gggCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGcc                                 <  1:470577/119‑1 (MQ=255)                                                                                                                       gggCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATc             >  1:187408/1‑139 (MQ=255)                                                                                                                        ggCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGca                                                                                                        <  1:281793/47‑1 (MQ=255)                                                                                                                        ggCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGca                                                                                                        >  2:281793/1‑47 (MQ=255)                                                                                                                        ggCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTa                        <  1:1471536/127‑1 (MQ=255)                                                                                                                        ggCGCTATGATCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTa                        >  2:1471536/1‑127 (MQ=255)                                                                                                                                  tCCGCCGCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGc  <  1:649127/139‑1 (MQ=255)                                                                                                                                      ccgCTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCg                                                               >  2:835469/1‑74 (MQ=255)                                                                                                                                         |                                                                                                                                     CGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATC‑TGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGC  >  NC_000913/3271796‑3272063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑