| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 340,089 | G→A | intergenic (+346/‑76) | yahG → / → yahI | DUF1116 family protein/carbamate kinase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 340,089 | 0 | G | A | 93.8% | 89.5 / ‑3.3 | 32 | intergenic (+346/‑76) | yahG/yahI | DUF1116 family protein/carbamate kinase‑like protein |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (16/14); total (17/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCC > NC_000913/339974‑340221 | tACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAg < 2:971547/139‑1 (MQ=255) cGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg > 2:1301474/1‑139 (MQ=255) gTGGCTCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg < 2:996762/135‑1 (MQ=255) gTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGAGAAGCGTAGCGCATCAgg > 1:996762/1‑135 (MQ=255) gagaATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg < 1:1301474/139‑1 (MQ=255) cgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAg > 2:1078639/1‑139 (MQ=255) cAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGAc > 1:803468/1‑139 (MQ=255) ttGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCa > 2:684811/1‑84 (MQ=255) ttGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCa < 1:684811/84‑1 (MQ=255) ttGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa > 2:276728/1‑136 (MQ=255) ttGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa < 1:276728/136‑1 (MQ=255) tGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAcc > 2:97792/1‑109 (MQ=255) tGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAcc < 1:97792/109‑1 (MQ=255) tGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAagag > 1:1198802/1‑139 (MQ=255) ggcgTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATc < 1:107326/96‑1 (MQ=255) ggcgTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATc > 2:107326/1‑96 (MQ=255) ggcgTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCg < 1:1078639/139‑1 (MQ=255) cgTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGtt > 1:651816/1‑139 (MQ=255) aaCGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCg > 1:34213/1‑133 (MQ=255) aaCGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCg < 2:34213/133‑1 (MQ=255) aaCGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCa < 2:803468/139‑1 (MQ=255) ttAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg < 2:1059861/57‑1 (MQ=255) ttAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg > 1:1059861/1‑57 (MQ=255) cGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa < 2:1250448/70‑1 (MQ=255) cGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa > 1:1250448/1‑70 (MQ=255) cGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTa > 1:612214/1‑139 (MQ=255) gcgcGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa > 2:1226068/1‑130 (MQ=255) gcgcGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa < 1:1226068/130‑1 (MQ=255) cgAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaaca > 2:166681/1‑85 (MQ=255) cgAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaaca < 1:166681/85‑1 (MQ=255) cgAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATa > 2:708165/1‑139 (MQ=255) aTTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTCTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACgcc < 2:1198802/139‑1 (MQ=255) | TACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCC > NC_000913/339974‑340221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |