| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,970,015 | T→C | W329R (TGG→CGG) | wzzE → | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,970,015 | 0 | T | C | 100.0% | 219.8 / NA | 63 | W329R (TGG→CGG) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (30/33); total (30/33) | |||||||||||
AGAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTG > NC_000913/3969881‑3970148 | aGAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCgggg > 2:698767/1‑139 (MQ=255) aTCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCAt < 2:322207/139‑1 (MQ=255) cGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTg > 2:777263/1‑139 (MQ=255) ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTc > 2:1393547/1‑139 (MQ=255) ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTc > 1:241092/1‑139 (MQ=255) tGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCa > 1:706833/1‑129 (MQ=255) tGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCa < 2:706833/129‑1 (MQ=255) ttAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggggggg < 1:987759/139‑2 (MQ=255) ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCgg > 1:1604765/1‑139 (MQ=255) aaTGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCt > 1:356246/1‑139 (MQ=255) tGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGgtgt < 1:1504243/139‑1 (MQ=255) ccAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCa < 1:871608/139‑1 (MQ=255) cAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCAGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGc < 1:335016/128‑1 (MQ=255) cAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCAGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGc > 2:335016/1‑128 (MQ=255) cccTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTa > 1:835464/1‑138 (MQ=255) cccTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTa < 2:835464/138‑1 (MQ=255) gATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACccgc > 1:746952/1‑139 (MQ=255) ccGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACccgccg < 1:1393547/139‑1 (MQ=255) gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGACGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTg > 2:226938/1‑139 (MQ=255) cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGgt > 1:368121/1‑118 (MQ=255) cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGgt < 2:368121/118‑1 (MQ=255) gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 2:1604765/139‑1 (MQ=255) gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 2:1271746/139‑1 (MQ=255) gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 1:698767/139‑1 (MQ=255) gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCCGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCA‑GGGGGCCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 1:1133086/139‑1 (MQ=255) ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 1:162054/139‑1 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggggggg < 2:272268/89‑2 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggggggg > 1:272268/1‑88 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑g > 1:1582836/1‑83 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑g < 2:1582836/83‑1 (MQ=255) cTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa > 1:79815/1‑126 (MQ=255) cTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa < 2:79815/126‑1 (MQ=255) cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGt < 2:904487/67‑1 (MQ=255) cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGt > 1:904487/1‑67 (MQ=255) cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAgc > 1:1089611/1‑139 (MQ=255) ccGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑gggggggc > 1:107385/1‑72 (MQ=255) ccGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑gggggggc < 2:107385/74‑3 (MQ=255) aagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAg > 1:170767/1‑139 (MQ=255) aagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAg > 1:854104/1‑139 (MQ=255) ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt > 2:35183/1‑139 (MQ=255) ggTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcg > 1:683834/1‑139 (MQ=255) tAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 1:867046/98‑1 (MQ=255) tAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc > 2:867046/1‑98 (MQ=255) aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa > 1:858179/1‑99 (MQ=255) aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa < 2:858179/99‑1 (MQ=255) aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCg > 1:125193/1‑139 (MQ=255) aCGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGaa < 2:241092/139‑1 (MQ=255) gtcgtGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGaa > 2:298985/1‑137 (MQ=255) gtcgtGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGaa < 1:298985/137‑1 (MQ=255) gCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 1:732673/75‑1 (MQ=255) gCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc > 2:732673/1‑75 (MQ=255) ccTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGTTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgtg > 1:103766/1‑139 (MQ=255) tgatgaTTATGCGGGGCATTGCCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa < 2:1089611/139‑1 (MQ=255) gatgatTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTac < 2:746952/139‑1 (MQ=255) gatTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga < 1:226938/139‑1 (MQ=255) gatTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga < 2:125193/139‑1 (MQ=255) atTATGCGGGGCATTGTCG‑GGGGGGTTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac < 2:103766/139‑1 (MQ=255) atTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac < 2:170767/139‑1 (MQ=255) atTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac < 2:854104/139‑1 (MQ=255) ttATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgact < 1:777263/139‑1 (MQ=255) tATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGgtgt < 1:778136/40‑1 (MQ=255) tATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGgtgt > 2:778136/1‑40 (MQ=255) tATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgactg > 2:195778/1‑139 (MQ=255) | AGAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTG > NC_000913/3969881‑3970148 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |