Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,821,867	(A)8→7	intergenic (‑248/+51)	chbB ← / ← osmE	N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,821,860	0	A	.	100.0%	243.9 / NA	57	intergenic (‑241/+58)	chbB/osmE	N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (34/23);  total (34/23)

ACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTA  >  NC_000913/1821738‑1821990                                                                                                                           |                                                                                                                                   aCGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAAt                                                                                                                   >  2:658961/1‑139 (MQ=255)               aGCAATAGTATTTCTTTGACCTTATAACTTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                     >  1:136063/1‑139 (MQ=255)               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                     >  2:779349/1‑139 (MQ=255)               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                     >  2:105936/1‑139 (MQ=255)               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                     >  2:124982/1‑139 (MQ=255)               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                     >  1:715592/1‑139 (MQ=255)               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                     >  2:142417/1‑139 (MQ=255)               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                     >  2:208168/1‑139 (MQ=255)               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                     >  2:33765/1‑139 (MQ=255)               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                     >  1:435812/1‑139 (MQ=255)               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                     >  2:552989/1‑139 (MQ=255)               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                     >  2:605205/1‑139 (MQ=255)               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgt                                                                                                      <  1:628420/138‑1 (MQ=255)               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgt                                                                                                      >  2:628420/1‑138 (MQ=255)               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGACAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGtt                                                                                                           >  2:618669/1‑133 (MQ=255)               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGACAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGtt                                                                                                           <  1:618669/133‑1 (MQ=255)                    tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTAc                                                                                                >  2:373963/1‑139 (MQ=255)                      gTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTc                                                                                              <  1:64139/139‑1 (MQ=255)                           tCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCt                                                                                         >  1:388894/1‑139 (MQ=255)                                      ataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAAt                                                                              >  2:463487/1‑139 (MQ=255)                                       taAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCt                                                                                         <  1:762590/127‑1 (MQ=255)                                       taAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCt                                                                                         >  2:762590/1‑127 (MQ=255)                                                tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGgc                                                                                                                        >  2:147291/1‑87 (MQ=255)                                                tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGgc                                                                                                                        <  1:147291/87‑1 (MQ=255)                                                 ctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                   <  2:435812/139‑1 (MQ=255)                                                 ctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                   <  1:124982/139‑1 (MQ=255)                                                   ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                 <  1:552989/139‑1 (MQ=255)                                                              ggTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATc                                                      >  2:684897/1‑139 (MQ=255)                                                               gTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCa                                                     <  1:33765/139‑1 (MQ=255)                                                               gTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCa                                                     <  1:142417/139‑1 (MQ=255)                                                                  aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                  >  2:141287/1‑139 (MQ=255)                                                                    gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                <  1:684897/139‑1 (MQ=255)                                                                       ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCGAGCTTTCAGACGAATTGATTACTTCCCAGCCTGTGGATCAGTGTCGTa                                             >  1:27811/1‑139 (MQ=255)                                                                         tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                   <  2:555205/115‑1 (MQ=255)                                                                         tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                   >  1:555205/1‑115 (MQ=255)                                                                          ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc                                          >  2:146761/1‑139 (MQ=255)                                                                          ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc                                          >  2:102686/1‑139 (MQ=255)                                                                          ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc                                          >  1:328614/1‑139 (MQ=255)                                                                              acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCTGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                      <  2:388894/139‑1 (MQ=255)                                                                              acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                      <  2:328614/139‑1 (MQ=255)                                                                              acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                      <  1:652121/139‑1 (MQ=255)                                                                               caTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGcac                                     >  2:524010/1‑139 (MQ=255)                                                                                 tttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTAGTCa                                                                                             >  1:512863/1‑81 (MQ=255)                                                                                 tttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCa                                                                                             <  2:512863/81‑1 (MQ=255)                                                                                  ttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTc                                                                                              >  1:542539/1‑79 (MQ=255)                                                                                  ttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTc                                                                                              <  2:542539/79‑1 (MQ=255)                                                                                    cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTc                                >  2:266782/1‑139 (MQ=255)                                                                                     gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                   <  2:781884/103‑1 (MQ=255)                                                                                     gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                   >  1:781884/1‑103 (MQ=255)                                                                                      ggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTg                              <  1:105936/139‑1 (MQ=255)                                                                                       gCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGa                             <  1:141287/139‑1 (MQ=255)                                                                                                             tAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc       <  1:266782/139‑1 (MQ=255)                                                                                                               aGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCg     <  2:715592/139‑1 (MQ=255)                                                                                                                  aCTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa  >  2:385933/1‑139 (MQ=255)                                                                                                                  aCTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa  >  1:99968/1‑139 (MQ=255)                                                                                                                      aGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                 >  1:153652/1‑72 (MQ=255)                                                                                                                      aGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                 <  2:153652/72‑1 (MQ=255)                                                                                                                           |                                                                                                                                   ACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTA  >  NC_000913/1821738‑1821990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑