| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,484,897 | (C)6→7 | coding (524/3594 nt) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,484,891 | 1 | . | C | 95.5% | 150.7 / 0.1 | 44 | coding (518/3594 nt) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (20/22); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAC > NC_000913/2484754‑2485020 | ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c > 2:215059/1‑139 (MQ=255) cgccgcCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑ccc > 1:716100/1‑139 (MQ=255) ttAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACg < 2:278391/139‑1 (MQ=255) ataatGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGTGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAgg < 1:492871/139‑1 (MQ=255) taatGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGt < 2:189648/139‑1 (MQ=255) tGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAAt > 2:534518/1‑139 (MQ=255) cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCA‑CAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATtcat > 2:51069/1‑139 (MQ=255) aTTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATtcat > 1:452079/1‑139 (MQ=255) gctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCatcaa > 1:147254/1‑139 (MQ=255) ctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCTCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCatcaat > 1:574656/1‑139 (MQ=255) ccaaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCCAC‑CAGACGAGGTAATTCATCAATCATTTc > 1:607017/1‑139 (MQ=255) aaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCaa < 2:147254/139‑1 (MQ=255) accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCaaa > 1:767019/1‑139 (MQ=255) ttGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGca > 2:108360/1‑139 (MQ=255) ccTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtct < 2:83495/139‑1 (MQ=255) ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACg > 2:362658/1‑97 (MQ=255) ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACg < 1:362658/97‑1 (MQ=255) cGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa < 2:527010/139‑1 (MQ=255) cGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa < 1:534518/139‑1 (MQ=255) cTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAt > 1:532520/1‑139 (MQ=255) ccaccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc < 2:334343/139‑1 (MQ=255) cccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAgg > 1:38955/1‑139 (MQ=255) cccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCACTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAgg < 1:215059/139‑1 (MQ=255) ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGc > 2:398551/1‑139 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt > 1:726268/1‑139 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt < 2:716100/139‑1 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt > 2:137485/1‑139 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct < 2:726268/139‑1 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct > 2:574989/1‑139 (MQ=255) gACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAg < 1:601850/139‑1 (MQ=255) ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa > 2:569729/1‑89 (MQ=255) ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa < 1:569729/89‑1 (MQ=255) ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt > 2:289286/1‑139 (MQ=255) ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCC‑CGCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt < 2:532520/139‑1 (MQ=255) cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTggg > 2:533260/1‑139 (MQ=255) ttACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAACTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc < 1:574989/139‑1 (MQ=255) aCCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAAACGAGGTAATTCATCAATCATTTCCAAAAGCAACTATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcac < 2:607017/139‑1 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa < 1:108360/139‑1 (MQ=255) aGTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAt < 1:137485/139‑1 (MQ=255) gTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct > 1:187005/1‑113 (MQ=255) gTAAATATTGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct < 2:187005/113‑1 (MQ=255) tGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa > 2:583668/1‑98 (MQ=255) tGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa < 1:583668/98‑1 (MQ=255) tGCTCGGGTAGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTaa > 1:265342/1‑139 (MQ=255) aGCAAATTACCCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAc < 1:51069/139‑1 (MQ=255) aGCAAATTACCCCC‑C‑CAGACCAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAc < 1:149419/139‑1 (MQ=255) | TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCC‑C‑CAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAC > NC_000913/2484754‑2485020 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |