Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	247,461	G→A	*250* (TAG→TAA)	yafL →	putative lipoprotein and C40 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	247,461	0	G	A	100.0%	97.3 / NA	32	*250* (TAG→TAA)	yafL	putative lipoprotein and C40 family peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/17);  total (15/17)

TATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCT  >  NC_000913/247327‑247587                                                                                                                                       |                                                                                                                               tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACg                                                                                                                            >  2:760769/1‑139 (MQ=255)     tGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACgataa                                                                                                                        >  1:694343/1‑135 (MQ=255)                         gAGTCGCCACGTACCGGCGAAACCCTTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg                                                                                                   <  1:695385/139‑1 (MQ=255)                         gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg                                                                                                   <  2:330733/139‑1 (MQ=255)                         gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg                                                                                                   <  2:694343/139‑1 (MQ=255)                         gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg                                                                                                   <  2:747214/139‑1 (MQ=255)                             cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGt                                                                                               >  2:70861/1‑139 (MQ=255)                              gCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGAATCCGGCAGtt                                                                                              >  2:522916/1‑139 (MQ=255)                                      ccGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAg                                                                                      <  2:324321/139‑1 (MQ=255)                                         gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTg                                                                                   >  2:576937/1‑139 (MQ=255)                                         gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTg                                                                                   >  2:749233/1‑139 (MQ=255)                                         gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTg                                                                                   >  2:242867/1‑139 (MQ=255)                                                       aTAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCt                                                                     >  1:517802/1‑139 (MQ=255)                                                         aaGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTgg                                                                   <  1:522916/139‑1 (MQ=255)                                                          aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                  <  1:576937/139‑1 (MQ=255)                                                             cGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGcgcg                                                               <  1:242867/139‑1 (MQ=255)                                                                     cGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCa                                                       <  1:70861/139‑1 (MQ=255)                                                                      gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                      >  2:338183/1‑139 (MQ=255)                                                                            ttCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGTAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTAc                                                >  2:184502/1‑139 (MQ=255)                                                                                    ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                        >  1:207749/1‑139 (MQ=255)                                                                                    ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                        >  2:279082/1‑139 (MQ=255)                                                                                      aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                  <  2:594344/111‑1 (MQ=255)                                                                                      aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                  >  1:594344/1‑111 (MQ=255)                                                                                          ttttttGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAatat                                  >  2:177514/1‑139 (MQ=255)                                                                                               tGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCg                             <  1:760769/139‑1 (MQ=255)                                                                                                gggCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGa                            <  1:749233/139‑1 (MQ=255)                                                                                                 ggCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAg                           <  1:184502/139‑1 (MQ=255)                                                                                                          aGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                  <  1:721693/139‑1 (MQ=255)                                                                                                                   aCGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATaaa         <  2:405883/139‑1 (MQ=255)                                                                                                                        agagACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCa                                                                                                        <  2:469342/39‑1 (MQ=18)                                                                                                                        agagACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCa                                                                                                        >  1:469342/1‑39 (MQ=18)                                                                                                                          agaCGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCt  <  2:233005/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                               TATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCT  >  NC_000913/247327‑247587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑