Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,447,869	Δ1 bp	coding (3758/3780 nt)	tamB →	translocation and assembly module for autotransporter export, inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,447,867	0	T	.	100.0%	299.6 / NA	73	coding (3756/3780 nt)	tamB	translocation and assembly module for autotransporter export, inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (41/32);  total (41/32)

CAGCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTG  >  NC_000913/4447729‑4447998                                                                                                                                           |                                                                                                                                    cAGCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAt                                                                                                                                     >  1:950758/1‑139 (MQ=255)   gCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAttg                                                                                                                                   <  2:263863/139‑2 (MQ=255)    cGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAt                                                                                                                                     >  1:1663977/1‑136 (MQ=255)    cGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAt                                                                                                                                     <  2:1663977/136‑1 (MQ=255)     ggCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAttgct                                                                                                                                 <  2:727383/139‑4 (MQ=255)              ttGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGtt                                                                                                                       <  2:552088/139‑1 (MQ=255)                     gTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGttt                                                                                                                >  1:1251481/1‑139 (MQ=255)                      tCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGtttt                                                                                                               >  2:575341/1‑139 (MQ=255)                      tCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGtttt                                                                                                               >  1:440248/1‑139 (MQ=255)                           aaGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACaa                                                                                                          >  2:1211581/1‑139 (MQ=255)                                        cGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATTAGTTCGCGTTTTAACAATGCGAATATTTGt                                                                                             >  2:877934/1‑139 (MQ=255)                                        cGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGt                                                                                             >  1:1612854/1‑139 (MQ=255)                                           gggTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTa                                                                                          <  2:1251481/139‑1 (MQ=255)                                                tatTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCa                                                                                     >  1:1645904/1‑139 (MQ=255)                                                       ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACg                                                                              >  1:1238049/1‑139 (MQ=255)                                                                    cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAttgc                                                                                                                                  >  2:550928/1‑73 (MQ=255)                                                                    cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAttgc                                                                                                                                  <  1:550928/75‑3 (MQ=255)                                                                    cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                 >  1:569093/1‑139 (MQ=255)                                                                    cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                 >  2:1731057/1‑139 (MQ=255)                                                                    cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTT‑CGCCACAAACAAGGCa                                                                >  2:13764/1‑139 (MQ=255)                                                                    cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGAGTAGACCAGGCACTGGA‑TTGCTCTATCAGATCGAGTTTTAACAATGCGAATATTTGTCGACGGCAGTTTACGCCACAAACAAGGc                                                                 >  1:1508980/1‑139 (MQ=255)                                                                      cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                               <  1:965671/139‑1 (MQ=255)                                                                      cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                               <  2:950758/139‑1 (MQ=255)                                                                      cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                               >  2:1605953/1‑139 (MQ=255)                                                                      cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                               <  2:1645904/139‑1 (MQ=255)                                                                      cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                               <  2:891449/139‑1 (MQ=255)                                                                      cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTAGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                               >  1:1497926/1‑139 (MQ=255)                                                                           tacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTc                                                          <  2:1612854/139‑1 (MQ=255)                                                                           tacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTc                                                          <  2:425707/139‑1 (MQ=255)                                                                               ttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                                 >  2:1465942/1‑96 (MQ=255)                                                                               ttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                                 <  1:1465942/96‑1 (MQ=255)                                                                                 aTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCATTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGa                                                    >  1:431145/1‑139 (MQ=255)                                                                                 aTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACgg                                                                                       <  1:1039252/104‑1 (MQ=255)                                                                                 aTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACgg                                                                                       >  2:1039252/1‑104 (MQ=255)                                                                                  tCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                   <  1:1211581/139‑1 (MQ=255)                                                                                     ccTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAc                                                <  1:1731057/139‑1 (MQ=255)                                                                                       tGATGCCTAAGCTATATCTGGAAGCCGTCTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTCTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCa                                              >  1:222913/1‑139 (MQ=255)                                                                                        gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                             >  2:1360843/1‑139 (MQ=255)                                                                                          tGCCTAAGCTATATCTGGAAGCCGTGTCTAGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATg                                           <  2:1497926/139‑1 (MQ=255)                                                                                            ccTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCTAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                         <  1:1284278/139‑1 (MQ=255)                                                                                            actagacTATATTTGGAACCTGTGTCAAGTATAGACCAGGCACTGGA‑TTGCTCTAGCAGGTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                         <  1:877934/133‑1 (MQ=255)                                                                                              tAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCa                                       >  2:1334731/1‑139 (MQ=255)                                                                                                aGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGt                                     >  1:1577200/1‑139 (MQ=255)                                                                                                     tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                                 >  1:1706302/1‑74 (MQ=255)                                                                                                     tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                                 <  2:1706302/74‑1 (MQ=255)                                                                                                     tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                           <  1:574664/112‑1 (MQ=255)                                                                                                     tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                           >  2:574664/1‑112 (MQ=255)                                                                                                     tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTg                                >  1:303423/1‑139 (MQ=255)                                                                                                      atCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGtt                                                                                                                       >  2:724806/1‑51 (MQ=255)                                                                                                      atCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGtt                                                                                                                       <  1:724806/51‑1 (MQ=255)                                                                                                      atCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCca                                                                           >  2:1164989/1‑95 (MQ=255)                                                                                                      atCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCca                                                                           <  1:1164989/95‑1 (MQ=255)                                                                                                           gAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTaac                                                                                                            >  2:895004/1‑55 (MQ=255)                                                                                                           gAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTaac                                                                                                            <  1:895004/57‑3 (MQ=255)                                                                                                           gAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTaa                                                                                                             <  2:1564901/56‑2 (MQ=255)                                                                                                           gAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTaa                                                                                                             >  1:1564901/1‑55 (MQ=255)                                                                                                           gAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCa                                                         >  1:1008130/1‑108 (MQ=255)                                                                                                           gAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCa                                                         <  2:1008130/108‑1 (MQ=255)                                                                                                           gAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                          >  1:173427/1‑139 (MQ=255)                                                                                                           gAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                          >  2:47067/1‑139 (MQ=255)                                                                                                            aaGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGtt                                                                                                                       >  1:776691/1‑45 (MQ=255)                                                                                                            aaGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGtt                                                                                                                       <  2:776691/45‑1 (MQ=255)                                                                                                            aaGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAtt                         >  1:1592769/1‑139 (MQ=255)                                                                                                             aGCCGTGTCTGGCGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTc                                                          <  1:313772/105‑1 (MQ=255)                                                                                                             aGCCGTGTCTGGCGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTc                                                          >  2:313772/1‑105 (MQ=255)                                                                                                               ccGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                      >  2:1447844/1‑139 (MQ=255)                                                                                                                  tgtCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTa                   >  1:134465/1‑139 (MQ=255)                                                                                                                   gtCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTAt                  <  1:575341/139‑1 (MQ=255)                                                                                                                   gtCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTAt                  >  1:24216/1‑139 (MQ=255)                                                                                                                      tGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGa               <  2:569093/139‑1 (MQ=255)                                                                                                                           tAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACt          <  2:134465/139‑1 (MQ=255)                                                                                                                             gACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCAATTTCAGTATCGATAACTAc        <  2:1341471/139‑1 (MQ=255)                                                                                                                              aCCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTT‑CGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa      <  1:13764/139‑1 (MQ=255)                                                                                                                                cAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAg                                                                   <  2:897519/77‑1 (MQ=255)                                                                                                                                cAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg     <  2:1577200/139‑1 (MQ=255)                                                                                                                                cAGGCACTGGA‑TTGCTCTATCAGTTCGAGCTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAg                                                                   >  1:897519/1‑77 (MQ=255)                                                                                                                                  ggCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGtt   >  1:968815/1‑139 (MQ=255)                                                                                                                                   gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCAc                                                        >  1:362809/1‑85 (MQ=255)                                                                                                                                   gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCAc                                                        <  2:362809/85‑1 (MQ=255)                                                                                                                                   gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg  >  2:1424657/1‑139 (MQ=255)                                                                                                                                           |                                                                                                                                    CAGCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTG  >  NC_000913/4447729‑4447998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑