Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,432,994	(C)6→7	coding (567/1125 nt)	smf ←	DNA recombination‑mediator A family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,432,988	1	.	C	100.0%	196.8 / NA	55	G191G (GGC→GGG)	smf	DNA recombination‑mediator A family protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (30/25);  total (30/25)

GTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTACACCAT  >  NC_000913/3432854‑3433115                                                                                                                                        |                                                                                                                                 gTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑‑cccc                                                                                                                             >  1:1524041/1‑139 (MQ=255)    aCCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑‑ccccccc                                                                                                                          <  2:1524041/139‑2 (MQ=255)     ccAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑‑ccccccct                                                                                                                         >  1:638625/1‑137 (MQ=255)          gAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCa                                                                                                                     >  2:1449231/1‑139 (MQ=255)            aCCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAg                                                                                                                   >  2:452845/1‑139 (MQ=255)                cTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGa                                                                                                               >  2:1306829/1‑139 (MQ=255)                        aGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGc                                                                                                       <  1:1207613/139‑1 (MQ=255)                             cTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑‑cccc                                                                                                                             >  2:642155/1‑111 (MQ=255)                             cTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑‑cccc                                                                                                                             <  1:642155/111‑1 (MQ=255)                                   ccaccaGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCAt                                                                                            <  2:638625/139‑1 (MQ=255)                                     accaGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGa                                                                                          <  1:63728/139‑1 (MQ=255)                                        aGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcgg                                                                                       >  2:728205/1‑139 (MQ=255)                                        aGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcgg                                                                                       <  1:625259/139‑1 (MQ=255)                                          tACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcggcg                                                                                     >  2:445631/1‑139 (MQ=255)                                          tACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcggcg                                                                                     <  1:445631/139‑1 (MQ=255)                                                 ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGa                                                                              <  1:456787/139‑1 (MQ=255)                                                 ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGa                                                                              >  1:158397/1‑139 (MQ=255)                                                 ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGa                                                                              <  1:1449231/139‑1 (MQ=255)                                                  tACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                                             >  1:766066/1‑139 (MQ=255)                                                   aCTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                                            <  1:237209/139‑1 (MQ=255)                                                         aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGa                                                                              <  1:971569/132‑1 (MQ=255)                                                         aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGa                                                                              >  2:971569/1‑132 (MQ=255)                                                         aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCa                                                                                                         >  2:710749/1‑104 (MQ=255)                                                         aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCa                                                                                                         <  1:710749/104‑1 (MQ=255)                                                         aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcgg                                                                                       <  2:371095/122‑1 (MQ=255)                                                         aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcgg                                                                                       >  1:371095/1‑122 (MQ=255)                                                         aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTAtt                                                                      >  1:394267/1‑139 (MQ=255)                                                              tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc                                                                 >  1:128603/1‑139 (MQ=255)                                                              tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc                                                                 >  1:399684/1‑139 (MQ=255)                                                               gATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTcc                                                                >  2:1459197/1‑139 (MQ=255)                                                                   aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTc                                                            >  2:885551/1‑139 (MQ=255)                                                                   aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTc                                                            <  1:1306829/139‑1 (MQ=255)                                                                   aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑‑ccccc                                                                                                                            <  1:1085984/74‑1 (MQ=255)                                                                   aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑‑ccccc                                                                                                                            >  2:1085984/1‑74 (MQ=255)                                                                     gCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc                                                          <  2:179583/139‑1 (MQ=255)                                                                      cGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTcccc                                                         >  1:1703619/1‑139 (MQ=255)                                                                        aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                                             <  2:59090/117‑1 (MQ=255)                                                                        aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                                             >  1:59090/1‑117 (MQ=255)                                                                        aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑‑cccccc                                                                                                                           <  1:1406368/70‑1 (MQ=255)                                                                        aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑‑cccccc                                                                                                                           >  2:1406368/1‑70 (MQ=255)                                                                                      aaTTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGa                                         <  2:158397/139‑1 (MQ=255)                                                                                        ttGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACg                                       <  1:1459197/139‑1 (MQ=255)                                                                                           tAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑‑ccccccc                                                                                                                          >  1:499992/1‑51 (MQ=255)                                                                                           tAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑‑ccccccc                                                                                                                          <  2:499992/52‑2 (MQ=255)                                                                                            aaGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCAt                                   >  1:562336/1‑139 (MQ=255)                                                                                                  ggggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                               >  1:116177/1‑105 (MQ=255)                                                                                                  ggggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                               <  2:116177/105‑1 (MQ=255)                                                                                                  ggggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCt                             >  2:775324/1‑139 (MQ=255)                                                                                                  ggggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCt                             >  1:516240/1‑139 (MQ=255)                                                                                                  ggggTGGAACATCGAGGGGAAATTCCGAGACGAGAGAGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCt                             >  2:1419673/1‑139 (MQ=255)                                                                                                   gggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGa                                                                                          <  2:1658009/77‑1 (MQ=255)                                                                                                   gggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGa                                                                                          >  1:1658009/1‑77 (MQ=255)                                                                                                     gTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTa                          <  2:128603/139‑1 (MQ=255)                                                                                                     gTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTa                          >  1:1437547/1‑139 (MQ=255)                                                                                                        gAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTc                                                            <  2:582430/102‑1 (MQ=255)                                                                                                        gAACATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTc                                                            >  1:582430/1‑102 (MQ=255)                                                                                                         aaCATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGa                                                                                          >  2:120280/1‑71 (MQ=255)                                                                                                         aaCATCGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGa                                                                                          <  1:120280/71‑1 (MQ=255)                                                                                                              cGAGGGGAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                                            >  2:257737/1‑80 (MQ=255)                                                                                                              cGAGGGGAAATTCCGAGAAGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                                            <  1:257737/80‑1 (MQ=255)                                                                                                                 ggggAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTAt              <  2:394267/139‑1 (MQ=255)                                                                                                                  gggAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATg             <  1:775324/139‑1 (MQ=255)                                                                                                                   ggAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATgc            >  1:1787967/1‑139 (MQ=255)                                                                                                                   ggAAATTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATgc            >  2:1424765/1‑139 (MQ=255)                                                                                                                      aaTTCCGAGACGAGAGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCt         <  1:452845/139‑1 (MQ=255)                                                                                                                             agacgagaGCGC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTACACCAt  >  2:811448/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                 GTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTACACCAT  >  NC_000913/3432854‑3433115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑