| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,511,746 | (A)8→9 | coding (599/906 nt) | yhfZ ← | putative DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,511,738 | 1 | . | A | 98.2% | 170.6 / ‑3.8 | 54 | coding (607/906 nt) | yhfZ | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (31/22); total (32/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAG > NC_000913/3511601‑3511870 | cggcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑a > 2:375360/1‑139 (MQ=255) gcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaa > 1:1617386/1‑139 (MQ=255) cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaa > 1:441125/1‑139 (MQ=255) tctAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaaaaca < 2:390949/139‑4 (MQ=255) aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATg < 2:3595/139‑1 (MQ=255) cATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGAt > 1:252249/1‑139 (MQ=255) ggTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGAtttt > 1:502647/1‑139 (MQ=255) tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCt > 2:657586/1‑139 (MQ=255) gTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGAt > 1:967368/1‑139 (MQ=255) tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATccgccg < 2:502647/139‑1 (MQ=255) tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATccgccg < 2:1617386/139‑1 (MQ=255) gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAAACA‑TCGGTCATGATTTTCTGAt < 1:1348336/129‑1 (MQ=255) gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAAACA‑TCGGTCATGATTTTCTGAt > 2:1348336/1‑129 (MQ=255) gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACgg < 2:1034188/139‑1 (MQ=255) cGCATAGACATCGCCTTTAACAATCAGTTGAAAACTCTCGTAATACTAAAAATCGCCGCGTTTCACTCCGCTTCCCCCT‑AAAAAAACCACCCGATCCCG‑TTTTCTGCTCCGCCGAACGGTTATTCACCCacacacctct > 1:284025/1‑133 (MQ=255) cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAAc < 1:1039947/115‑1 (MQ=255) cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAAc > 2:1039947/1‑115 (MQ=255) tCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACg > 1:1503956/1‑139 (MQ=255) tCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCg < 2:967368/139‑1 (MQ=255) ccTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCgg < 2:15408/80‑1 (MQ=255) ccTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCgg > 1:15408/1‑80 (MQ=255) ccTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGAt < 2:606466/95‑1 (MQ=255) ccTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGAt > 1:606466/1‑95 (MQ=255) cTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt < 1:657586/139‑1 (MQ=255) tttAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc < 2:441125/139‑1 (MQ=255) tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc > 1:835296/1‑139 (MQ=255) gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg > 1:33561/1‑139 (MQ=255) gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg > 1:1423309/1‑139 (MQ=255) cGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg < 2:1503956/139‑1 (MQ=255) tctcGTGTTAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACt > 1:1196363/1‑139 (MQ=255) tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑a > 2:343374/1‑42 (MQ=255) tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑a < 1:343374/42‑1 (MQ=255) gTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg < 2:1259231/139‑1 (MQ=255) tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTa < 2:1280145/82‑1 (MQ=255) tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTa > 1:1280145/1‑82 (MQ=255) tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGc > 2:1717326/1‑139 (MQ=255) aaGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc < 2:599911/139‑1 (MQ=255) aaGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc < 2:835296/139‑1 (MQ=255) agagaTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCaa > 2:1230740/1‑139 (MQ=255) agagaTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCaa > 2:563683/1‑139 (MQ=255) agagaTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAACCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCaa > 1:1359428/1‑139 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa > 1:555928/1‑139 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa > 2:955027/1‑139 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa > 1:232344/1‑139 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa > 2:1523746/1‑139 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa > 1:1260957/1‑139 (MQ=255) cTCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCCCGCGCCTCCCGTCTGCCGCCTCCCCTTTACGCCCAATCACCTCGTGCTCGCCACCGTCGgtgt > 1:1626082/1‑139 (MQ=255) tCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg < 1:229219/106‑1 (MQ=255) tCGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg > 2:229219/1‑106 (MQ=255) cGTTCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGc < 2:555928/139‑1 (MQ=255) tCCACATCACTACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGc < 1:1523746/139‑1 (MQ=255) tACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc < 1:143434/102‑1 (MQ=255) tACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc > 2:143434/1‑102 (MQ=255) tACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTTGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa > 2:811623/1‑139 (MQ=255) tACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc < 2:395116/112‑1 (MQ=255) tACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc < 2:692248/112‑1 (MQ=255) tACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc > 1:692248/1‑112 (MQ=255) tACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc > 1:395116/1‑112 (MQ=255) tACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa > 2:298843/1‑139 (MQ=255) tACCGCCA‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa > 1:481523/1‑139 (MQ=255) tACCGCC‑‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAg > 1:1389902/1‑139 (MQ=255) | CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AAAAAAAACA‑TCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAG > NC_000913/3511601‑3511870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |