Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,245,600	G→A	*147* (TAG→TAA)	ycgY →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,600	0	G	A	96.9%	198.3 / ‑1.0	64	coding (441/441 nt)	ycgY	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (29/33);  minor base . (1/1);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01

AGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGT  >  NC_000913/1245466‑1245717                                                                                                                                        |                                                                                                                       aGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTaaaaaa                                                                                                                    >  2:579132/1‑139 (MQ=255)   ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTaaaaaaaa                                                                                                                  >  2:1393342/1‑139 (MQ=255)   ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTaaaaaaaa                                                                                                                  >  1:538362/1‑139 (MQ=255)   ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTaaaaaaaa                                                                                                                  >  1:1761770/1‑139 (MQ=255)   ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTaaaaaaaa                                                                                                                  >  2:732936/1‑139 (MQ=255)   ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCACGACTTGAAAAACCGTCTGTGTaaaaaaaa                                                                                                                  >  1:75359/1‑139 (MQ=255)    cAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAAc                                                                                                                 <  2:1771060/139‑1 (MQ=255)    cAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAAc                                                                                                                 <  1:533370/139‑1 (MQ=255)      aCGTCGGCGTAAATCACCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                               <  2:285067/139‑1 (MQ=255)       cGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCAt                                                                                                              >  1:973109/1‑139 (MQ=255)       cGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTAAGGCAAACATTTGCGCAATACTTCCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                               <  1:952158/139‑1 (MQ=255)       cGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTAAGGCAAACATTTGCGCAATACTTCCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                               >  2:952158/1‑139 (MQ=255)        gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATa                                                                                                             >  2:272557/1‑139 (MQ=255)           ggCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGcc                                                                                                          <  2:1467383/139‑2 (MQ=255)           ggCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          <  1:410758/139‑1 (MQ=255)             cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTaaaa                                                                                                                      >  2:472080/1‑125 (MQ=255)             cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          >  2:1784084/1‑137 (MQ=255)             cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          <  1:1784084/137‑1 (MQ=255)             cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTAt                                                                                                        >  2:1054916/1‑139 (MQ=255)                  aTCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGgtgt                                                                                                   >  2:1331080/1‑139 (MQ=255)                              tgaAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACa                                                                                      <  1:272557/139‑1 (MQ=255)                                 aaGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACg                                                                                   <  1:579132/139‑1 (MQ=255)                                     gagactcTTACCCGTAGAAATGATGCTATCTTCGGCGATCACGTATGGCAAACATTTGCTAAATATTT‑CCGGCCAGAATTGGAAAAACCGTCTGTGTAAAAAAAACTATATCTATGGTGTTAT‑CTGCCGACACCGCCAt                                                                               <  1:533501/133‑1 (MQ=255)                                       aaCACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGT‑‑AAAAAACCATAGCTATGgt                                                                                                     <  1:1764890/114‑1 (MQ=255)                                       aaCACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGT‑‑AAAAAACCATAGCTATGgt                                                                                                     >  2:1764890/1‑114 (MQ=255)                                            ttACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          <  2:574851/106‑1 (MQ=255)                                            ttACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          >  1:574851/1‑106 (MQ=255)                                            ttACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGgaga                                                                        <  1:1331080/139‑1 (MQ=255)                                              aCCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAg                                                                      <  1:732936/139‑1 (MQ=255)                                              aCCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAg                                                                      >  1:1502288/1‑139 (MQ=255)                                                  gTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACa                                                                                      >  1:802038/1‑119 (MQ=255)                                                  gTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACa                                                                                      <  2:802038/119‑1 (MQ=255)                                                    aGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAAt                                                                >  1:817421/1‑139 (MQ=255)                                                     gAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATg                                                               >  1:912336/1‑139 (MQ=255)                                                      aaaTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGa                                                              <  2:973109/139‑1 (MQ=255)                                                      aaaTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGa                                                              >  1:1525593/1‑139 (MQ=255)                                                       aaTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGAc                                                             <  2:480025/139‑1 (MQ=255)                                                        aTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACg                                                            <  2:1502288/139‑1 (MQ=255)                                                             gCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTc                                                       >  1:1147333/1‑139 (MQ=255)                                                              cTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          <  2:1636420/88‑1 (MQ=255)                                                              cTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          >  1:1636420/1‑88 (MQ=255)                                                                 tCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAAc                                                                                                                 >  2:117781/1‑78 (MQ=255)                                                                 tCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAAc                                                                                                                 <  1:117781/78‑1 (MQ=255)                                                                  cTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGt                                                  <  2:1333842/139‑1 (MQ=255)                                                                      ggCAACCACGTATGGCAAACATTTGCGCAATGCTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                              <  2:1525593/139‑1 (MQ=255)                                                                      ggCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTTT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGGTGTCGGAGTAAgc                                              <  2:966258/139‑1 (MQ=255)                                                                      ggCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                              >  2:229925/1‑139 (MQ=255)                                                                           ccACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          >  2:1643133/1‑75 (MQ=255)                                                                           ccACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          <  1:1643133/75‑1 (MQ=255)                                                                               gTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTaa                                     <  2:281549/139‑1 (MQ=255)                                                                                            ttGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTtgtg                        >  1:296953/1‑139 (MQ=255)                                                                                             tGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                               >  2:201994/1‑52 (MQ=255)                                                                                             tGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                               <  1:201994/52‑1 (MQ=255)                                                                                             tGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGc                       <  1:229925/139‑1 (MQ=255)                                                                                             tGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGc                       >  2:1303134/1‑139 (MQ=255)                                                                                                gcAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAG‑AGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCctct                   <  1:1054916/139‑1 (MQ=255)                                                                                                  aaTACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCtt                  <  2:790636/139‑1 (MQ=255)                                                                                                    tACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTg                >  1:526313/1‑139 (MQ=255)                                                                                                          cccGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAAc                                                                                    <  2:845704/66‑1 (MQ=255)                                                                                                          cccGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAAc                                                                                    >  1:845704/1‑66 (MQ=255)                                                                                                          cccGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGa           <  2:538362/139‑1 (MQ=255)                                                                                                          cccGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGa           >  2:857182/1‑139 (MQ=255)                                                                                                          cccGCCAGGACTTGAAAAACCGTCTGAGTAAAAAAAAACATGGCTATGATGTTAT‑CTGCCCAGAACCCAACCCGCAGAACCCGAATGACATCCTCGGAGTACGCGCCGGATAAAGTATGAAATGTGccgatctgtgtgt           >  1:3523/1‑128 (MQ=255)                                                                                                             gCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAggg        >  2:460130/1‑139 (MQ=255)                                                                                                               cAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGtt      >  1:953100/1‑139 (MQ=255)                                                                                                               cAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTC‑TTGGTTGAGGGTTg     >  1:1740827/1‑139 (MQ=255)                                                                                                                 ggACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACg                                                            >  1:227241/1‑83 (MQ=255)                                                                                                                 ggACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACg                                                            <  2:227241/83‑1 (MQ=255)                                                                                                                 ggACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGc    >  2:385754/1‑139 (MQ=255)                                                                                                                   aCTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGcg   <  2:1761770/139‑1 (MQ=255)                                                                                                                   aCTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGcgt  <  2:296953/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                       AGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGT  >  NC_000913/1245466‑1245717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑