Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,478,092	A→G	V82V (GTA→GTG)	dsdX →	D‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,478,092	0	A	G	100.0%	216.0 / NA	61	V82V (GTA→GTG)	dsdX	D‑serine transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/34);  total (27/34)

GTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCT  >  NC_000913/2477964‑2478227                                                                                                                                  |                                                                                                                                        gTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCg                                                                                                                               <  2:1648124/139‑1 (MQ=255)     gAACGCTGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGTAATTGGCGTAACGCTGGGTTCCCTCGCAGCGTTTATCGGCCTTGGCACGATACTGTG‑AAAAATGATGGAAGTGTCCGGGGCCGCaga                                                                                                                           <  1:1089013/139‑1 (MQ=255)     gAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCaga                                                                                                                           <  2:983689/139‑1 (MQ=255)     gAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCaga                                                                                                                           <  1:211583/139‑1 (MQ=255)          atgatgGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCagaaagaa                                                                                                                      <  1:924890/139‑1 (MQ=255)              tgGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTgg                                                                                                                  <  2:1079860/139‑1 (MQ=255)                ggCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTc                                                                                                                <  1:731041/139‑1 (MQ=255)                            cTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAAc                                                                                                    >  1:1610079/1‑139 (MQ=255)                             tGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACg                                                                                                   >  1:773124/1‑139 (MQ=255)                                   tGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCg                                                                                             <  2:773124/139‑1 (MQ=255)                                         aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTTGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAAAGCTGCCGCTGGCt                                                                                       >  2:1224674/1‑139 (MQ=255)                                         aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCt                                                                                       >  2:1438700/1‑139 (MQ=255)                                              aTTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAg                                                                                  >  2:1008394/1‑139 (MQ=255)                                               ttGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGt                                                                                 <  2:1517159/139‑1 (MQ=255)                                               ttGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGt                                                                                 >  1:569601/1‑139 (MQ=255)                                               ttGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGt                                                                                 <  2:1233015/139‑1 (MQ=255)                                                tGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGtt                                                                                <  1:1224674/139‑1 (MQ=255)                                                  aaaGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGa                                                                              <  2:1610079/139‑1 (MQ=255)                                                    aGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATg                                                                            >  2:763518/1‑139 (MQ=255)                                                      tGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGc                                                                                                                                 >  2:1325967/1‑84 (MQ=255)                                                      tGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGc                                                                                                                                 <  1:1325967/84‑1 (MQ=255)                                                          aTTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTa                                                                      <  2:788624/139‑1 (MQ=255)                                                                  aaCGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTg                                                              >  1:1169322/1‑139 (MQ=255)                                                                   aCGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTgg                                                             <  2:1169322/139‑1 (MQ=255)                                                                         gggTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGcc                                                       >  1:1005541/1‑139 (MQ=255)                                                                              ccTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGAtt                                                  >  2:1550559/1‑139 (MQ=255)                                                                              ccTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGAtt                                                  >  2:1434035/1‑139 (MQ=255)                                                                                   cAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTa                                                                      <  2:589769/114‑1 (MQ=255)                                                                                   cAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTa                                                                      >  1:589769/1‑114 (MQ=255)                                                                                     gCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCa                                           <  1:1008394/139‑1 (MQ=255)                                                                                          tATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                      <  1:763518/139‑1 (MQ=255)                                                                                          tATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                      <  1:1550559/139‑1 (MQ=255)                                                                                          tATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                      <  2:1005541/139‑1 (MQ=255)                                                                                           aTCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCt                                                                                                               >  2:40385/1‑65 (MQ=255)                                                                                           aTCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCt                                                                                                               <  1:40385/65‑1 (MQ=255)                                                                                           aTCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGc                                     >  1:834899/1‑139 (MQ=255)                                                                                                   tGGCACGATACTGGGAAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGa                                                                                                             <  2:1560742/60‑1 (MQ=255)                                                                                                   tGGCACGATACTGGGAAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGa                                                                                                             >  1:1560742/1‑60 (MQ=255)                                                                                                   tGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCg                                                                                                                               >  2:677412/1‑41 (MQ=255)                                                                                                   tGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCg                                                                                                                               <  1:677412/41‑1 (MQ=255)                                                                                                   tGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTgg                                                             <  2:1451065/107‑1 (MQ=255)                                                                                                   tGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTgg                                                             >  1:1451065/1‑107 (MQ=255)                                                                                                   tGGCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCtgtttgtt                             <  2:834899/139‑1 (MQ=255)                                                                                                   tGGAACGATACTGGG‑AAAAATGATGGAAGTGTGCGGGGCCGCAGAAAGAATTGTTCTGACACTTCAACGCTGCCGCTGGCTTTTAGTTGATGTCTTTATGGTGCTGCTTGGCCTGATTTGTGGCATCACGCtgtttgtt                             >  1:323974/1‑139 (MQ=255)                                                                                                    ggCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                      >  1:1316724/1‑129 (MQ=255)                                                                                                    ggCACGATACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                      <  2:1316724/129‑1 (MQ=255)                                                                                                          aTACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAATGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATGATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGt                        >  1:1582342/1‑137 (MQ=255)                                                                                                          aTACTGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGt                        <  2:1582342/137‑1 (MQ=255)                                                                                                              tGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTtg                                                <  1:459955/109‑1 (MQ=255)                                                                                                              tGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTtg                                                >  2:459955/1‑109 (MQ=255)                                                                                                              tGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                      >  1:324228/1‑119 (MQ=255)                                                                                                              tGGG‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                      <  2:324228/119‑1 (MQ=255)                                                                                                               ggg‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGcgtc                 <  1:1434035/139‑1 (MQ=255)                                                                                                                gg‑AAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTCGGcgtcg                <  1:1017895/139‑1 (MQ=255)                                                                                                                    aaaaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGc             >  2:1246138/1‑139 (MQ=255)                                                                                                                      aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGtt                                                                                <  2:1680190/70‑1 (MQ=255)                                                                                                                      aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGtt                                                                                >  1:1680190/1‑70 (MQ=255)                                                                                                                      aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCt                                    <  2:353661/114‑1 (MQ=255)                                                                                                                      aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCt                                    >  1:353661/1‑114 (MQ=255)                                                                                                                      aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACGTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTa           >  1:1269274/1‑139 (MQ=255)                                                                                                                               aGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCct  <  2:1269274/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        GTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGG‑AAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCT  >  NC_000913/2477964‑2478227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑