Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,794,015	G→A	*427* (TAG→TAA)	gabT →	4‑aminobutyrate aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,794,015	0	G	A	100.0%	140.4 / NA	44	*427* (TAG→TAA)	gabT	4‑aminobutyrate aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/20);  total (24/20)

TAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGG  >  NC_000913/2793880‑2794136                                                                                                                                         |                                                                                                                          tAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAacgc                                                                                                                        <  1:999422/139‑1 (MQ=255)               ttctCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCgg                                                                                                          >  2:2745/1‑139 (MQ=255)                      cTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTCGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGAc                                                                                                   >  2:1081429/1‑139 (MQ=255)                         cGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCt                                                                                                >  1:1052361/1‑139 (MQ=255)                              cGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAAcgccg                                                                                                                      >  1:266671/1‑112 (MQ=255)                              cGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAAcgccg                                                                                                                      <  2:266671/112‑1 (MQ=255)                                tATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTc                                                                                         <  2:1739206/139‑1 (MQ=255)                                          tgctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTc                                                                                    >  2:1135652/1‑134 (MQ=255)                                          tgctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGCAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTc                                                                                    <  1:1135652/134‑1 (MQ=255)                                            ctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTa                                                                             >  1:1345002/1‑139 (MQ=255)                                                    ccTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATc                                                                     >  1:1002160/1‑139 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAAcgccg                                                                                                                      >  2:368790/1‑80 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAAcgccg                                                                                                                      <  1:368790/80‑1 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGTCTACGGGGATc                                                                     >  1:1469005/1‑129 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGTCTACGGGGATc                                                                     <  2:1469005/129‑1 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                           >  2:727033/1‑139 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                           >  2:1102154/1‑139 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                           >  2:1432583/1‑139 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                           >  2:1433724/1‑139 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                           >  2:167756/1‑139 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                           >  2:51046/1‑139 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                           >  2:546281/1‑139 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                           >  1:1416037/1‑139 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                           <  1:338039/139‑1 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                           >  2:1007345/1‑139 (MQ=255)                                                                             gCTCAGATCCGTCAGGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGAt                                                                      <  2:603264/114‑1 (MQ=255)                                                                             gCTCAGATCCGTCAGGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGAt                                                                      >  1:603264/1‑114 (MQ=255)                                                                              cTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTAcgccgc                                           <  1:1433724/139‑1 (MQ=255)                                                                              cTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTAcgccgc                                           <  1:167756/139‑1 (MQ=255)                                                                                    tCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCTAGGTCGGATAAGGCGTTTACGCCGCATCCGa                                     <  1:2745/139‑1 (MQ=255)                                                                                         cA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATc                                <  2:1416037/139‑1 (MQ=255)                                                                                                  ggAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGAc                                                                                                   <  1:639545/64‑1 (MQ=255)                                                                                                  ggAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGAc                                                                                                   >  2:639545/1‑64 (MQ=255)                                                                                                     gaTCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAAc                     <  2:1002160/139‑1 (MQ=255)                                                                                                         atcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTAcg                                               <  1:685406/109‑1 (MQ=255)                                                                                                         atcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTAcg                                               >  2:685406/1‑109 (MQ=255)                                                                                                          tcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaa                <  1:746854/139‑1 (MQ=255)                                                                                                          tcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaa                <  2:1052361/139‑1 (MQ=255)                                                                                                           caGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaa                >  2:1431114/1‑138 (MQ=255)                                                                                                           caGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaa                <  1:1431114/138‑1 (MQ=255)                                                                                                             gCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCAGa                                     >  1:44787/1‑115 (MQ=255)                                                                                                             gCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCAGa                                     <  2:44787/115‑1 (MQ=255)                                                                                                                        gatgaGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTgg  <  1:727033/139‑1 (MQ=255)                                                                                                                            aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTa                                                                             <  2:1125461/60‑1 (MQ=255)                                                                                                                            aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTa                                                                             >  1:1125461/1‑60 (MQ=255)                                                                                                                                         |                                                                                                                          TAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCA‑GGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGG  >  NC_000913/2793880‑2794136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑