| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,800,475 | G→A | *110* (TAG→TAA) | ygaM → | putative membrane‑anchored DUF883 family ribosome‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,800,475 | 0 | G | A | 96.3% | 159.9 / ‑1.6 | 54 | *110* (TAG→TAA) | ygaM | putative membrane‑anchored DUF883 family ribosome‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (26/26); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01 | |||||||||||
CCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGC > NC_000913/2800346‑2800606 | ccAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAAt < 1:420791/139‑1 (MQ=255) ccGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGCTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATACTAAATACGC‑GTCttt < 1:1198500/139‑1 (MQ=255) aGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATc > 2:1584019/1‑139 (MQ=255) gCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTgg > 2:129242/1‑132 (MQ=255) gCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTgg < 1:129242/132‑1 (MQ=255) cGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATcac > 1:1625843/1‑139 (MQ=255) cGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATcac > 2:1283165/1‑139 (MQ=255) ggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGata > 1:1012303/1‑73 (MQ=255) ggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGata < 2:1012303/73‑1 (MQ=255) cagcagCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATcc < 2:66774/139‑1 (MQ=255) agcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAg > 1:1225046/1‑94 (MQ=255) agcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAg < 2:1225046/94‑1 (MQ=255) agcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATccc > 1:877215/1‑139 (MQ=255) tGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcc > 2:1193053/1‑139 (MQ=255) gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGGaaa < 2:1221252/54‑1 (MQ=39) gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaa > 1:1221252/1‑54 (MQ=255) aggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa < 1:1554934/139‑1 (MQ=255) gtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACg < 2:901261/139‑1 (MQ=255) ttttATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCa > 1:1750458/1‑139 (MQ=255) tCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaaa > 2:725597/1‑39 (MQ=255) tCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaaa < 1:725597/39‑1 (MQ=255) ggCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCtt < 1:212260/88‑1 (MQ=255) ggCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCtt > 2:212260/1‑88 (MQ=255) ggCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCt < 1:106246/130‑1 (MQ=255) ggCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCt > 2:106246/1‑130 (MQ=255) ggCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAg > 2:653865/1‑139 (MQ=255) cgcACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa < 1:463606/124‑1 (MQ=255) cgcACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa > 2:463606/1‑124 (MQ=255) aCTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTaa > 1:1152900/1‑139 (MQ=255) ttAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATcc < 1:72712/104‑1 (MQ=255) ttAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATcc > 2:72712/1‑104 (MQ=255) aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCtt > 1:13312/1‑46 (MQ=255) aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCtt < 2:13312/46‑1 (MQ=255) aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc < 1:1193053/139‑1 (MQ=255) aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc < 2:1625843/139‑1 (MQ=255) cATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATcac < 2:159403/91‑1 (MQ=255) cATGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATcac > 1:159403/1‑91 (MQ=255) tggccAAATCCTTATGGAAAAATGATTATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACt < 1:1523839/135‑1 (MQ=255) tGCGCAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACt > 1:90122/1‑139 (MQ=255) gcgcAAATCGTAATGCAAAAATGATAATAAATAcgc < 2:302283/36‑1 (MQ=255) gcgcAAATCGTAATGCAAAAATGATAATAAATAcgc > 1:302283/1‑36 (MQ=38) gcgcAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTg > 1:1372727/1‑75 (MQ=255) gcgcAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTg < 2:1372727/75‑1 (MQ=255) gcgcAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACg > 2:425297/1‑111 (MQ=255) gcgcAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACg < 1:425297/111‑1 (MQ=255) gcgcAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa > 2:1481652/1‑139 (MQ=255) gcgcAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa > 1:683882/1‑139 (MQ=255) gcgcAAATCGTAATGCAAAAATGATAAT‑AATAAGCGGCCTTTAACCCGAAAGCCTGTCTTGGGGGTTTTTTTTTCCTTGTGGAATAAAAAAATTCCCCCTCCCCCTTCACCCTCTTATCAAGGGTATTTTGGACAAcat > 2:1523839/1‑137 (MQ=255) cgcAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt < 1:1584019/139‑1 (MQ=255) cgcAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt < 2:683882/139‑1 (MQ=255) cgcAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt < 2:1152900/139‑1 (MQ=255) cgcAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGAGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt < 1:1283165/139‑1 (MQ=255) gcAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGa > 2:289552/1‑48 (MQ=255) gcAAATCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGa < 1:289552/48‑1 (MQ=255) aaTCGTAATGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc < 2:1750458/139‑1 (MQ=255) | CCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGC‑GTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGC > NC_000913/2800346‑2800606 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |