Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,024,576	(T)5→4	coding (609/624 nt)	rcsA →	transcriptional regulator of colanic acid capsular biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,024,572	0	T	.	100.0%	312.7 / NA	76	coding (605/624 nt)	rcsA	transcriptional regulator of colanic acid capsular biosynthesis
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (35/41);  total (35/41)

AAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGT  >  NC_000913/2024437‑2024705                                                                                                                                        |                                                                                                                                      aaaTCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTCCCATGTCGTCCGACTGACGGATAATGTGACTAATGGTAtttt                                                                                                                                    >  1:1199661/1‑139 (MQ=255) aaaTCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTAtttt                                                                                                                                    >  2:769743/1‑139 (MQ=255)        tGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACa                                                                                                                            <  2:1328139/139‑1 (MQ=255)         gACCAAATGA‑‑ATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATgc                                                                                                                         >  2:205902/1‑139 (MQ=255)            cAAATGAATATCAAAGCCAAGACCATTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATgcg                                                                                                                        <  2:1199661/139‑1 (MQ=255)                tGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTaa                                                                                                                    <  2:1462686/139‑1 (MQ=255)                         aaGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTg                                                                                                           >  1:1614212/1‑139 (MQ=255)                               aGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACtggt                                                                                                     <  2:1289764/139‑1 (MQ=255)                                       tCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTccc                                                                                             <  1:769743/139‑1 (MQ=255)                                             cATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGt                                                                                       <  1:500459/139‑1 (MQ=255)                                             cATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGt                                                                                       >  1:540943/1‑139 (MQ=255)                                                aaaGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAg                                                                                    >  2:909212/1‑139 (MQ=255)                                                 aaGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAgg                                                                                   <  2:1614212/139‑1 (MQ=255)                                                 aaGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAgg                                                                                   <  2:540943/139‑1 (MQ=255)                                                      aaTATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGaa                                                                              <  1:1353990/139‑1 (MQ=255)                                                       atatTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAAt                                                                             >  1:636309/1‑139 (MQ=255)                                                             aaCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATgcg                                                                                                                        >  2:1275667/1‑90 (MQ=255)                                                             aaCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATgcg                                                                                                                        <  1:1275667/90‑1 (MQ=255)                                                              aCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAgg                                                                                   >  2:1188293/1‑126 (MQ=255)                                                              aCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAgg                                                                                   <  1:1188293/126‑1 (MQ=255)                                                              aCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTg                                                                                >  2:1474386/1‑129 (MQ=255)                                                              aCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTg                                                                                <  1:1474386/129‑1 (MQ=255)                                                                   aGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACa                                                                                                                            >  2:573485/1‑80 (MQ=255)                                                                   aGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACa                                                                                                                            <  1:573485/80‑1 (MQ=255)                                                                    gATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTAct                                                                    >  1:1312728/1‑135 (MQ=255)                                                                    gATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTAct                                                                    <  2:1312728/135‑1 (MQ=255)                                                                       cAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCttt                                                             <  2:1021667/139‑1 (MQ=255)                                                                         aaaCGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTtct                                                           >  1:504686/1‑139 (MQ=255)                                                                          aaCGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTtctc                                                          >  2:170984/1‑139 (MQ=255)                                                                                taataaACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACaaa                                                    >  1:575411/1‑139 (MQ=255)                                                                                    aaaCAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATa                                                <  1:1481595/139‑1 (MQ=255)                                                                                       cAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAgg                                                                                   <  2:1517990/101‑1 (MQ=255)                                                                                       cAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAgg                                                                                   >  1:1517990/1‑101 (MQ=255)                                                                                         ggTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTc                                           >  1:597627/1‑139 (MQ=255)                                                                                             aTCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACAtt                                                                                                              >  1:1200415/1‑68 (MQ=255)                                                                                             aTCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACAtt                                                                                                              <  2:1200415/68‑1 (MQ=255)                                                                                             aTCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGa                                                                         <  1:1382983/105‑1 (MQ=255)                                                                                             aTCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGa                                                                         >  2:1382983/1‑105 (MQ=255)                                                                                             aTCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGa                                       >  1:794376/1‑139 (MQ=255)                                                                                             aTCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGa                                       >  2:757683/1‑139 (MQ=255)                                                                                               cTACCATGTCGTCCGACTGACTGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATg                                     <  2:591384/139‑1 (MQ=255)                                                                                               cTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATg                                     <  2:636309/139‑1 (MQ=255)                                                                                               cTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATg                                     >  2:1489160/1‑139 (MQ=255)                                                                                                tACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGc                                    <  1:757683/139‑1 (MQ=255)                                                                                                    aTGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGtt                                                                                                 >  2:954520/1‑74 (MQ=255)                                                                                                    aTGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGtt                                                                                                 <  1:954520/74‑1 (MQ=255)                                                                                                    aTGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATg                                     <  1:168654/134‑1 (MQ=255)                                                                                                    aTGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATg                                     >  2:168654/1‑134 (MQ=255)                                                                                                    aTGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTg                                >  1:222998/1‑139 (MQ=255)                                                                                                    aTGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTg                                <  1:205902/139‑1 (MQ=255)                                                                                                     tGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATg                                     <  2:825955/133‑1 (MQ=255)                                                                                                     tGTCGTCCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATg                                     >  1:825955/1‑133 (MQ=255)                                                                                                        cgtcCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTc                            <  2:608593/139‑1 (MQ=255)                                                                                                          tcCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGaa                                                                              <  2:1059471/87‑1 (MQ=255)                                                                                                          tcCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGaa                                                                              >  1:1059471/1‑87 (MQ=255)                                                                                                          tcCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAACGATACCGCCCTGATGCCCTGCTTCAt                          >  2:775884/1‑139 (MQ=255)                                                                                                          tcCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCa                           >  2:278887/1‑138 (MQ=255)                                                                                                          tcCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCa                           <  1:278887/138‑1 (MQ=255)                                                                                                          tcCGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCAt                          <  1:254477/139‑1 (MQ=255)                                                                                                           ccGACTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGA‑TACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTa                        <  2:1029868/139‑1 (MQ=255)                                                                                                               cTGACGGATAATGTGACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAg                     >  2:45724/1‑139 (MQ=255)                                                                                                                            tgACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGt                                                                                       >  1:209685/1‑60 (MQ=255)                                                                                                                            tgACTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGt                                                                                       <  2:209685/60‑1 (MQ=255)                                                                                                                              aCTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTg                                                                                >  2:863176/1‑65 (MQ=255)                                                                                                                              aCTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTg                                                                                <  1:863176/65‑1 (MQ=255)                                                                                                                               cTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGtt                                                                                                 <  2:793189/47‑1 (MQ=255)                                                                                                                               cTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGtt                                                                                                 >  1:793189/1‑47 (MQ=255)                                                                                                                               cTAATGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCg     <  2:575411/139‑1 (MQ=255)                                                                                                                                 aaTGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCggg   <  2:504686/139‑1 (MQ=255)                                                                                                                                 aaTGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCggg   <  2:222998/139‑1 (MQ=255)                                                                                                                                 aaTGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCggg   <  1:1489160/139‑1 (MQ=255)                                                                                                                                  aTGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTccc                                                                                             >  2:555856/1‑48 (MQ=255)                                                                                                                                  aTGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTccc                                                                                             <  1:555856/48‑1 (MQ=255)                                                                                                                                  aTGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTtctc                                                          <  1:1354186/83‑1 (MQ=255)                                                                                                                                  aTGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGt  >  2:703843/1‑139 (MQ=255)                                                                                                                                  aTGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACTGGAGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTtctc                                                          >  2:1354186/1‑83 (MQ=255)                                                                                                                                   tGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACtgg                                                                                                      <  1:959042/38‑1 (MQ=255)                                                                                                                                   tGGTA‑TTTTGTCAACATGCGCTAACACATTCTGACtgg                                                                                                      >  2:959042/1‑38 (MQ=255)                                                                                                                                        |                                                                                                                                      AAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGT  >  NC_000913/2024437‑2024705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑