Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,860,426	Δ1 bp	intergenic (+10/+41)	pphB → / ← ygbI	serine/threonine‑specific protein phosphatase 2/DeoR family putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,860,425	0	T	.	100.0%	240.6 / NA	59	intergenic (+9/+42)	pphB/ygbI	serine/threonine‑specific protein phosphatase 2/DeoR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (32/27);  total (32/27)

CGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGT  >  NC_000913/2860293‑2860543                                                                                                                                     |                                                                                                                       cGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCtg                                                                                                                 >  2:998519/1‑139 (MQ=255)            attTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAg                                                                                                      <  1:312367/139‑1 (MQ=255)                  tatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAccc                                                                                                <  1:633955/139‑1 (MQ=255)                   atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCg                                                                                               >  2:455837/1‑139 (MQ=255)                            tatGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTg                                                                                      <  2:595194/139‑1 (MQ=255)                            tatGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTg                                                                                      >  2:3040/1‑139 (MQ=255)                             atgatgTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCtgt                                                                                                                >  2:1626050/1‑112 (MQ=255)                             atgatgTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCtgt                                                                                                                <  1:1626050/112‑1 (MQ=255)                              tgatgTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGctctt                                                                                                                     <  2:1623241/107‑4 (MQ=255)                              tgatgTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGctctt                                                                                                                     >  1:1623241/1‑104 (MQ=255)                                   tttGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCa                                                                               >  2:188498/1‑139 (MQ=255)                                     tGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAcc                                                                                                 <  2:1006553/119‑1 (MQ=255)                                     tGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCatt                                                                             >  1:148766/1‑139 (MQ=255)                                     tGATAACATTCAGAAGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTATATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAcc                                                                                                 >  1:1006553/1‑119 (MQ=255)                                       aTAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGct                                                                                                                        >  1:10193/1‑95 (MQ=255)                                       aTAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGct                                                                                                                        <  2:10193/95‑1 (MQ=255)                                           cATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCAt                                                                       >  1:1311738/1‑139 (MQ=255)                                           cATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCAt                                                                       >  2:1160492/1‑139 (MQ=255)                                             ttCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGc                                                                                                           <  1:37280/101‑1 (MQ=255)                                             ttCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGc                                                                                                           >  2:37280/1‑101 (MQ=255)                                                  aCGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGcc                                                                                <  2:516055/123‑1 (MQ=255)                                                  aCGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGcc                                                                                >  1:516055/1‑123 (MQ=255)                                                      tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCatta                                                                            >  2:357987/1‑123 (MQ=255)                                                      tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCatta                                                                            <  1:357987/123‑1 (MQ=255)                                                                 aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCAt                                                                                                         <  2:1453659/83‑1 (MQ=255)                                                                 aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCAt                                                                                                         >  1:1453659/1‑83 (MQ=255)                                                                 aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAagagag                                                 >  2:1030757/1‑139 (MQ=255)                                                                   ttATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGAt                                               <  1:1275200/139‑1 (MQ=255)                                                                   ttATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGAt                                               <  1:188498/139‑1 (MQ=255)                                                                        ttGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCa                                                                                    <  2:91495/97‑1 (MQ=255)                                                                        ttGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCa                                                                                    >  1:91495/1‑97 (MQ=255)                                                                           aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAACGTAATCTCATGC‑TCTTCtg                                                                                                                 >  1:648095/1‑65 (MQ=255)                                                                           aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCtg                                                                                                                 <  2:648095/65‑1 (MQ=255)                                                                           aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCa                                                                                    <  2:1509602/94‑1 (MQ=255)                                                                           aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCa                                                                                    >  1:1509602/1‑94 (MQ=255)                                                                           aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                       >  1:608932/1‑139 (MQ=255)                                                                           aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                       >  1:290804/1‑139 (MQ=255)                                                                           aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                       >  2:679519/1‑139 (MQ=255)                                                                             aCCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAccc                                                                                                <  2:576614/80‑1 (MQ=255)                                                                             aCCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAccc                                                                                                >  1:576614/1‑80 (MQ=255)                                                                                ggATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGct                                                                                                                        >  1:680476/1‑54 (MQ=255)                                                                                ggATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGct                                                                                                                        <  2:680476/54‑1 (MQ=255)                                                                                  aTCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcg                                >  1:700518/1‑139 (MQ=255)                                                                                     gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                              <  2:306803/106‑1 (MQ=255)                                                                                     gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                              >  1:306803/1‑106 (MQ=255)                                                                                             gcgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCa                     <  1:679519/139‑1 (MQ=255)                                                                                              cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCAc                    >  2:521893/1‑139 (MQ=255)                                                                                              cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCAc                    >  2:163675/1‑139 (MQ=255)                                                                                              cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCAc                    >  1:714509/1‑139 (MQ=255)                                                                                                ggcggcTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGc                                                                                                           <  2:880448/50‑1 (MQ=255)                                                                                                ggcggcTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGc                                                                                                           >  1:880448/1‑50 (MQ=255)                                                                                                ggcggcTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTg                                                                                      <  1:1528275/71‑1 (MQ=255)                                                                                                ggcggcTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTg                                                                                      >  2:1528275/1‑71 (MQ=255)                                                                                                ggcggcTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCActc                  >  1:479307/1‑139 (MQ=255)                                                                                                       gTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTCCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCa           <  1:998519/139‑1 (MQ=255)                                                                                                           ttttATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCATGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAAcc       <  2:290804/139‑1 (MQ=255)                                                                                                            tttATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcg      <  1:455837/139‑1 (MQ=255)                                                                                                                taaaataaaGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtcgt  <  2:714509/139‑1 (MQ=255)                                                                                                                taaaataaaGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtcgt  <  1:837870/139‑1 (MQ=255)                                                                                                                        agtaatCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCa                     >  1:1129431/1‑112 (MQ=255)                                                                                                                        agtaatCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTCCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCa                     <  2:1129431/112‑1 (MQ=255)                                                                                                                             tctcATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCa                     >  1:846555/1‑107 (MQ=255)                                                                                                                             tctcATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCa                     <  2:846555/107‑1 (MQ=255)                                                                                                                               tcATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtcgt  >  1:1147116/1‑124 (MQ=255)                                                                                                                               tcATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtcgt  <  2:1147116/124‑1 (MQ=255)                                                                                                                                     |                                                                                                                       CGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGT  >  NC_000913/2860293‑2860543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑