Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,309,006	(C)6→5	intergenic (‑82/+27)	nlpI ← / ← pnp	lipoprotein involved in osmotic sensitivity and filamentation/polynucleotide phosphorylase/polyadenylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,309,001	0	C	.	100.0%	244.8 / NA	60	intergenic (‑77/+32)	nlpI/pnp	lipoprotein involved in osmotic sensitivity and filamentation/polynucleotide phosphorylase/polyadenylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (32/28);  total (32/28)

CTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGAT  >  NC_000913/3308866‑3309130                                                                                                                                        |                                                                                                                                  ctacATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGtccc                                                                                                                                <  2:236162/136‑1 (MQ=255) ctacATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccc                                                                                                                                <  1:391728/136‑1 (MQ=255) ctacATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccc                                                                                                                                <  2:657628/136‑1 (MQ=255) ctacATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccc                                                                                                                                <  2:1491347/136‑1 (MQ=255) ctacATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccc                                                                                                                                >  1:1491347/4‑139 (MQ=255)   acATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGccccca                                                                                                                              >  2:357465/2‑138 (MQ=255)      tCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccccaccg                                                                                                                           >  1:1050893/1‑135 (MQ=255)      tCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccccaccg                                                                                                                           >  1:964418/1‑135 (MQ=255)         tGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTACTAACAAGGCGTCCTGCCCGGTTAAAAGcccccaccgcag                                                                                                                        >  2:221098/1‑132 (MQ=255)         tGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTACTAACAAGGCGTCCTGCCCGGTTAAAAGcccccaccgcag                                                                                                                        <  1:221098/139‑8 (MQ=255)           cAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCg                                                                                                                     >  2:449228/1‑139 (MQ=255)           cAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCg                                                                                                                     >  1:167805/1‑139 (MQ=255)           cAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCg                                                                                                                     >  1:948546/1‑139 (MQ=255)              gCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAg                                                                                                                  <  1:1323726/139‑1 (MQ=255)                  aaGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCa                                                                                                              <  1:357465/139‑1 (MQ=255)                   aGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCaa                                                                                                             >  2:581242/1‑139 (MQ=255)                       cTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATgg                                                                                                         >  1:610596/1‑139 (MQ=255)                            gCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAAcc                                                                                                    >  1:1413194/1‑139 (MQ=255)                               aCGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTa                                                                                                 >  2:1590503/1‑139 (MQ=255)                               aCGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTa                                                                                                 >  1:1242011/1‑139 (MQ=255)                                cGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                                <  2:964418/139‑1 (MQ=255)                                   aaCACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATg                                                                                                          >  2:877804/1‑126 (MQ=255)                                   aaCACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTCTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATg                                                                                                          <  1:877804/126‑1 (MQ=255)                                          gcgcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                             >  2:467061/1‑132 (MQ=255)                                          gcgcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                             <  1:467061/132‑1 (MQ=255)                                             cAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTcag                                                                                   >  1:576906/1‑139 (MQ=255)                                                          aTTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTc                                                                      <  1:174030/139‑1 (MQ=255)                                                           tttCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTcc                                                                     <  2:167805/139‑1 (MQ=255)                                                                cACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                           <  1:1609127/112‑1 (MQ=255)                                                                cACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                           >  2:1609127/1‑112 (MQ=255)                                                                    cccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgca                                                            >  2:903063/1‑139 (MQ=255)                                                                    cccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgca                                                            >  2:585536/1‑139 (MQ=255)                                                                        aaGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGcagcag                                                        >  1:776944/1‑139 (MQ=255)                                                                          gACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGt                                                      <  2:1242011/139‑1 (MQ=255)                                                                             cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGtgct                                                               <  1:527783/127‑1 (MQ=255)                                                                             cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGtgct                                                               >  2:527783/1‑127 (MQ=255)                                                                             cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTga                                                   >  2:449459/1‑139 (MQ=255)                                                                             cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑‑CCCCACCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                                <  1:561069/93‑1 (MQ=255)                                                                             cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑‑CCCCACCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                                >  2:561069/1‑93 (MQ=255)                                                                               cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGt                                                      <  2:547526/134‑1 (MQ=255)                                                                               cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGt                                                      >  1:547526/1‑134 (MQ=255)                                                                               cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgaga                                                 >  1:1223071/1‑139 (MQ=255)                                                                                  ttGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTg                                              <  1:581242/139‑1 (MQ=255)                                                                                     aatgaaCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTc                                           <  2:578021/139‑1 (MQ=255)                                                                                        gaaCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGt                                        <  2:576906/139‑1 (MQ=255)                                                                                          aCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCg                                      <  1:449459/139‑1 (MQ=255)                                                                                             tCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGCCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGctt                                   <  2:1050893/139‑1 (MQ=255)                                                                                                   tCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAACAGGTTGAGACTGCTCAGTCGCTTCTTTaa                             >  1:1269369/1‑139 (MQ=255)                                                                                                      cGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAggg                                                                                                                >  2:1316027/1‑53 (MQ=255)                                                                                                      cGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAggg                                                                                                                <  1:1316027/53‑1 (MQ=255)                                                                                                      cGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGc                          >  1:268913/1‑139 (MQ=255)                                                                                                         ttGCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCa                       <  2:776944/139‑1 (MQ=255)                                                                                                           gCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                           >  2:276752/1‑101 (MQ=255)                                                                                                           gCTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                           <  1:276752/101‑1 (MQ=255)                                                                                                            cTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCgg                                                                                                                    <  2:271746/43‑1 (MQ=255)                                                                                                            cTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCgg                                                                                                                    >  1:271746/1‑43 (MQ=255)                                                                                                            cTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGtgctgc                                                             >  1:122295/1‑98 (MQ=255)                                                                                                            cTAACAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGtgctgc                                                             <  2:122295/98‑1 (MQ=255)                                                                                                               aCAAGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGa                 <  1:449228/139‑1 (MQ=255)                                                                                                                 aaGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATa               <  2:1413194/139‑1 (MQ=255)                                                                                                                  aGGCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATAc              >  1:221916/1‑139 (MQ=255)                                                                                                                   ggCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgaga                                                 <  2:449463/103‑1 (MQ=255)                                                                                                                   ggCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgaga                                                 >  1:449463/1‑103 (MQ=255)                                                                                                                   ggCGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACg             >  1:196757/1‑139 (MQ=255)                                                                                                                     cGTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGc           >  2:1321736/1‑139 (MQ=255)                                                                                                                      gTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGa                     <  1:1468023/128‑1 (MQ=255)                                                                                                                      gTCCTGCCCGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGa                     >  2:1468023/1‑128 (MQ=255)                                                                                                                            cccGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCa                                          >  1:1454105/1‑101 (MQ=255)                                                                                                                            cccGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCa                                          <  2:1454105/101‑1 (MQ=255)                                                                                                                              cGGTTAAAAG‑CCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGAt  <  2:352199/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  CTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGAT  >  NC_000913/3308866‑3309130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑