| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,399,985 | (C)7→8 | coding (252/516 nt) | ogt ← | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,399,978 | 1 | . | C | 97.7% | 143.8 / ‑3.7 | 43 | coding (259/516 nt) | ogt | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (18/24); total (18/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAT > NC_000913/1399842‑1400100 | ggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc < 1:533569/139‑1 (MQ=255)ggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc < 1:79971/139‑1 (MQ=255)ggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc < 1:581052/139‑1 (MQ=255) cGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccc‑cccc < 1:1088323/139‑1 (MQ=255) aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccc‑cccccg > 1:721209/1‑137 (MQ=255) aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccc‑cccccg > 2:1147828/1‑137 (MQ=255) ccGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccc‑cccccgta < 2:653852/139‑5 (MQ=255) cGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccc‑cccccgtag < 1:411357/139‑6 (MQ=255) gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGc < 2:721209/139‑1 (MQ=255) gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTAAAATGGCGTCCCC‑CCCCGTAGc < 1:271687/139‑1 (MQ=255) gCACCAACGGCACACGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGa < 2:872533/139‑1 (MQ=255) cACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGaa > 1:727811/1‑139 (MQ=255) aCCAACGGCGCGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccc‑cccc < 2:729674/124‑1 (MQ=255) aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccc‑cccc > 1:729674/1‑124 (MQ=255) aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc > 2:1630446/1‑119 (MQ=255) aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc < 1:1630446/119‑1 (MQ=255) aaCGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGt < 1:1147828/139‑1 (MQ=255) aaCAGCACGCGCCGCGCCAGGACGGTCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGt < 1:1069006/139‑1 (MQ=255) cACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCaa < 1:96600/139‑1 (MQ=255) aCGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCaat > 2:1480366/1‑139 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTAt < 1:781157/116‑1 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTAt > 2:781157/1‑116 (MQ=255) gTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCt > 2:995128/1‑139 (MQ=255) ttGGCCGTAATGCATTTCCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt < 1:995128/139‑1 (MQ=255) ttGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt < 2:727811/139‑1 (MQ=255) ttGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt > 1:303899/1‑139 (MQ=255) tGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaaa < 2:766570/139‑1 (MQ=255) ttACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGc > 2:1318938/1‑139 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAg > 1:420684/1‑74 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAg < 2:420684/74‑1 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccc‑cccccg > 2:945940/1‑58 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccc‑cccccg < 1:945940/60‑3 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccc‑c < 1:896141/55‑1 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccc‑c > 2:896141/1‑55 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc < 1:941407/53‑1 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc < 1:1157262/53‑1 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc > 2:1157262/1‑53 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc > 2:941407/1‑53 (MQ=255) ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc < 1:590695/52‑1 (MQ=255) ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc > 2:590695/1‑52 (MQ=255) ggATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAg < 1:78958/139‑1 (MQ=255) gTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAATATATTCACGAAGCTTGTCGCTTAAACCGCCTGGa > 1:189688/1‑124 (MQ=255) gTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAATATATTCACGAAGCTTGTCGCTTAAACCGCCTGGa < 2:189688/124‑1 (MQ=255) gTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATg < 2:488538/139‑1 (MQ=255) gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAg < 2:291254/56‑1 (MQ=255) gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAg > 1:291254/1‑56 (MQ=255) gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCaata < 1:535646/66‑1 (MQ=255) gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCaata > 2:535646/1‑66 (MQ=255) tAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGa < 2:1415080/53‑1 (MQ=255) tAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGa < 1:1276398/53‑1 (MQ=255) tAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGa > 1:1415080/1‑53 (MQ=255) tAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGa > 2:1276398/1‑53 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCaat > 2:1100967/1‑63 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCaat < 1:1100967/63‑1 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa < 1:480380/108‑1 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGATAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa > 2:480380/1‑108 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCCACCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCa < 1:1001749/139‑1 (MQ=255) gtgtTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGa > 2:829742/1‑51 (MQ=255) gtgtTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGa < 1:829742/51‑1 (MQ=255) ttttCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcgc < 2:612429/132‑1 (MQ=255) ttttCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcgc > 1:612429/1‑132 (MQ=255) ttCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTAc < 1:1541570/63‑1 (MQ=255) ttCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTAc > 2:1541570/1‑63 (MQ=255) ttCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCg > 2:1218463/1‑139 (MQ=255) cgcgCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt < 1:1218463/139‑1 (MQ=255) | GGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAT > NC_000913/1399842‑1400100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |