| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,609,336 | (C)7→8 | intergenic (+13/‑78) | yjjG → / → prfC | dUMP phosphatase/peptide chain release factor RF‑3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,609,329 | 1 | . | C | 98.2% | 186.4 / ‑3.6 | 54 | intergenic (+6/‑85) | yjjG/prfC | dUMP phosphatase/peptide chain release factor RF‑3 |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (30/23); total (30/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
TCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACTTTT > NC_000913/4609193‑4609461 | tcGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cc < 1:1477876/139‑1 (MQ=255) aCGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGAt > 2:5641/1‑139 (MQ=255) tgAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCg < 1:5641/139‑1 (MQ=255) tGCACACCATCGCGTGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGtttt > 2:1327632/1‑139 (MQ=255) acCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTcaaca > 1:359324/1‑139 (MQ=255) tCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcg > 1:493564/1‑139 (MQ=255) cgcgAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgc > 1:779744/1‑139 (MQ=255) cgcgAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgc > 2:3116/1‑139 (MQ=255) cgcgAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgc > 1:374350/1‑139 (MQ=255) aCCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGAt < 2:1361706/139‑1 (MQ=255) aGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCt < 1:1327632/139‑1 (MQ=255) gCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTa < 1:277277/139‑1 (MQ=255) aTCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccc‑c > 2:208470/1‑73 (MQ=255) aTCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccc‑c > 2:1512834/1‑73 (MQ=255) aTCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccc‑c < 1:208470/73‑1 (MQ=255) aTCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccc‑c < 1:1512834/73‑1 (MQ=255) tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACt > 1:954171/1‑139 (MQ=255) tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTc > 2:432788/1‑139 (MQ=255) tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTc > 2:861847/1‑139 (MQ=255) tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTc < 1:861847/139‑1 (MQ=255) cgcgCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccc‑cccccgttg > 1:443870/1‑74 (MQ=255) cgcgCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccc‑cccccgttg < 2:443870/79‑6 (MQ=255) cgcCCACCTCGACCGTTTCTGCGTTGCTCGATCTGGAGCAGCGCCTGTGTAAACACTGATTGCCT‑‑CCCTCCTCGTTGATCGGTAAAATAGCCGCAATTTTTGGTTTTCAACAAGCGCTGTGCGATGCGGCTTACTGaag < 2:1367861/139‑1 (MQ=255) gcCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaa > 1:973026/1‑137 (MQ=255) gcCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaa < 2:973026/137‑1 (MQ=255) cACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga < 2:779744/139‑1 (MQ=255) cACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga < 2:374350/139‑1 (MQ=255) aCCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAAtt > 2:458852/1‑139 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGa > 1:219496/1‑139 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGa > 2:118336/1‑139 (MQ=255) ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCt > 2:450087/1‑49 (MQ=255) ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCt < 1:450087/49‑1 (MQ=255) tcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg < 1:432788/139‑1 (MQ=255) tGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga < 1:608072/119‑1 (MQ=255) tGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga > 2:608072/1‑119 (MQ=255) cACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggcg < 2:325463/96‑1 (MQ=255) cACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggcg > 1:325463/1‑96 (MQ=255) cGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTa < 1:118336/139‑1 (MQ=255) cGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTa < 1:458852/139‑1 (MQ=255) ggAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg > 2:1068591/1‑122 (MQ=255) ggAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg < 1:1068591/122‑1 (MQ=255) agcagcTCCTGTGTAAACACTGATTGCCT‑‑ccc‑cccc > 1:378152/1‑36 (MQ=255) agcagcTCCTGTGTAAACACTGATTGCCT‑‑ccc‑cccc < 2:378152/36‑1 (MQ=255) cTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTcc > 1:127870/1‑123 (MQ=255) cTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTcc < 2:127870/123‑1 (MQ=255) cTCCTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTg > 1:804126/1‑139 (MQ=255) ccTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGtt > 1:701073/1‑115 (MQ=255) ccTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGtt < 2:701073/115‑1 (MQ=255) cTGTGTAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCg < 2:493564/139‑1 (MQ=255) tgtAAACACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAg > 1:139754/1‑139 (MQ=255) aaCACTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGcgcc > 2:450037/1‑139 (MQ=255) acTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTcaa < 2:781319/56‑1 (MQ=255) acTGATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTcaa > 1:781319/1‑56 (MQ=255) gATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTc < 1:1427801/79‑1 (MQ=255) gATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTc > 2:1427801/1‑79 (MQ=255) gATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga > 1:673984/1‑86 (MQ=255) gATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga < 2:673984/86‑1 (MQ=255) gATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga < 2:508446/86‑1 (MQ=255) gATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga > 1:508446/1‑86 (MQ=255) gATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGtt < 2:595838/101‑1 (MQ=255) gATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGtt > 1:595838/1‑101 (MQ=255) gATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAg > 1:130619/1‑129 (MQ=255) gATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAg < 2:130619/129‑1 (MQ=255) gATTGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACtt > 2:1612131/1‑139 (MQ=255) ttGCCTC‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACtttt > 2:1554068/1‑139 (MQ=255) | TCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑CCC‑CCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACTTTT > NC_000913/4609193‑4609461 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |