Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,245,600	G→A	*147* (TAG→TAA)	ycgY →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,600	0	G	A	100.0%	207.0 / NA	63	*147* (TAG→TAA)	ycgY	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/36);  total (27/36)

CCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AGAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTG  >  NC_000913/1245468‑1245725                                                                                                                                      |                                                                                                                               ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑aaaaaaaa                                                                                                                          >  1:1498922/1‑139 (MQ=255)   aaCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAAcc                                                                                                                        >  1:1551676/1‑139 (MQ=255)    aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCa                                                                                                                       <  1:1220143/139‑1 (MQ=255)    aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGGATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCa                                                                                                                       <  2:575516/139‑1 (MQ=255)     cGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCAt                                                                                                                      <  2:126268/139‑1 (MQ=255)     cGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCAt                                                                                                                      <  1:1260075/139‑1 (MQ=255)     cGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCAt                                                                                                                      <  1:920051/139‑1 (MQ=255)      gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATa                                                                                                                     >  2:917980/1‑139 (MQ=255)      gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATa                                                                                                                     >  1:146739/1‑139 (MQ=255)         ggCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCt                                                                                                                  <  2:995814/139‑1 (MQ=255)         ggCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCt                                                                                                                  <  2:208669/139‑1 (MQ=255)         ggCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCt                                                                                                                  <  2:1141121/139‑1 (MQ=255)           cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCa                                                                                                                       <  1:724454/132‑1 (MQ=255)           cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCa                                                                                                                       >  2:724454/1‑132 (MQ=255)           cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTAt                                                                                                                >  2:1419984/1‑139 (MQ=255)           cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTAt                                                                                                                >  1:105054/1‑139 (MQ=255)            gTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATg                                                                                                               <  1:399010/139‑1 (MQ=255)                     cGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑Ct                                                                                                     <  1:451457/139‑1 (MQ=255)                               aaGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACg                                                                                           >  1:1504224/1‑139 (MQ=255)                               aaGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACg                                                                                           <  1:947981/139‑1 (MQ=255)                                          ttACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGgaga                                                                                <  1:1353997/139‑1 (MQ=255)                                           tACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑aaaa                                                                                                                              >  1:1331066/1‑93 (MQ=255)                                              ccGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑Ct                                                                                                     <  2:28016/114‑1 (MQ=255)                                              ccGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑Ct                                                                                                     >  1:28016/1‑114 (MQ=255)                                                      aTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACg                                                                    <  1:917980/139‑1 (MQ=255)                                                           gCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTc                                                               >  1:279872/1‑139 (MQ=255)                                                                    ggCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                                      >  2:1440593/1‑139 (MQ=255)                                                                     gCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                                      <  1:281287/139‑1 (MQ=255)                                                                      cAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgcgc                                                    <  2:279872/139‑1 (MQ=255)                                                                         ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgcgc                                                    >  2:1604184/1‑136 (MQ=255)                                                                         ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgcgc                                                    <  1:1604184/136‑1 (MQ=255)                                                                         ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTgg                                                 >  1:63678/1‑139 (MQ=255)                                                                           aCGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                               >  1:681535/1‑139 (MQ=255)                                                                           aCGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                               <  2:1551676/139‑1 (MQ=255)                                                                                    aaaCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTa                                                                                                                 <  1:1044561/65‑1 (MQ=255)                                                                                    aaaCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTa                                                                                                                 >  2:1044561/1‑65 (MQ=255)                                                                                    aaaCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGga                                                                                  <  1:949716/95‑1 (MQ=255)                                                                                    aaaCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTgg                                      >  2:532292/1‑139 (MQ=255)                                                                                    aaaCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTGAT‑CTGCCGACAACGCCATCCGga                                                                                  >  2:949716/1‑95 (MQ=255)                                                                                       cATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                               <  2:757648/127‑1 (MQ=255)                                                                                       cATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                               >  1:757648/1‑127 (MQ=255)                                                                                       cATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGgtg                                         <  2:723758/133‑1 (MQ=255)                                                                                       cATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGgtg                                         >  1:723758/1‑133 (MQ=255)                                                                                        aTTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTtg                                  <  1:434354/139‑1 (MQ=255)                                                                                          ttGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTtgtg                                <  2:63678/139‑1 (MQ=255)                                                                                           tGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                               >  2:1151129/1‑123 (MQ=255)                                                                                           tGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                               <  1:1151129/123‑1 (MQ=255)                                                                                           tGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGc                               <  1:1549222/139‑1 (MQ=255)                                                                                                 aTACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGa                                                                      >  1:817411/1‑94 (MQ=255)                                                                                                 aTACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGa                                                                      <  2:817411/94‑1 (MQ=255)                                                                                                  tACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAATCCATAGCt                                                                                                                  <  1:1076989/50‑1 (MQ=38)                                                                                                  tACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCt                                                                                                                  >  2:1076989/1‑50 (MQ=255)                                                                                                       cccGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATg                                                                                                               >  2:740102/1‑48 (MQ=255)                                                                                                       cccGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATg                                                                                                               <  1:740102/48‑1 (MQ=255)                                                                                                       cccGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCCTGCCGACAACGCCATCCgttgtcgg                                                                             >  2:460508/1‑75 (MQ=255)                                                                                                       cccGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCCTGCCGACAACGCCATCCgttgtcgg                                                                             <  1:460508/82‑8 (MQ=255)                                                                                                          gCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTa                                                                                                                 <  2:561000/43‑1 (MQ=255)                                                                                                          gCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTa                                                                                                                 >  1:561000/1‑43 (MQ=255)                                                                                                          gCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAggg                >  1:1510293/1‑139 (MQ=255)                                                                                                            cAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACaa                                                                                             >  1:983456/1‑60 (MQ=255)                                                                                                            cAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACaa                                                                                             <  2:983456/60‑1 (MQ=255)                                                                                                            cAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGtt              >  1:1015297/1‑139 (MQ=255)                                                                                                                     aaaaaCCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTg     <  2:146739/139‑1 (MQ=255)                                                                                                                      aaaaCCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGc    <  2:1015297/139‑1 (MQ=255)                                                                                                                        aaCCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTg  <  2:1510293/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                               CCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AGAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTG  >  NC_000913/1245468‑1245725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑