Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	247,461	G→A	*250* (TAG→TAA)	yafL →	putative lipoprotein and C40 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	247,461	0	G	A	100.0%	235.5 / NA	74	*250* (TAG→TAA)	yafL	putative lipoprotein and C40 family peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/38);  total (36/38)

TGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAA  >  NC_000913/247325‑247596                                                                                                                                         |                                                                                                                                        tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGa                                                                                                                                       >  1:1264962/1‑139 (MQ=255)   tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACg                                                                                                                                     >  1:973655/1‑139 (MQ=255)          gCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGc                                                                                                                             >  2:976145/1‑139 (MQ=255)             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACgat                                                                                                                                   <  2:30617/131‑3 (MQ=255)             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACgat                                                                                                                                   >  1:30617/1‑129 (MQ=255)             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGtt                                                                                                                          >  1:433837/1‑139 (MQ=255)              tGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGttt                                                                                                                         >  2:302709/1‑139 (MQ=255)              tGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGttt                                                                                                                         >  1:1297987/1‑139 (MQ=255)               gggCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTa                                                                                                                        >  2:998149/1‑139 (MQ=255)                ggCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTAc                                                                                                                       >  2:1340748/1‑139 (MQ=255)                    aTTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTAcgccg                                                                                                                   <  2:1264962/139‑1 (MQ=255)                     tttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTAcgccgc                                                                                                                  >  1:1377709/1‑139 (MQ=255)                      ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCa                                                                                                                 <  2:1377709/139‑1 (MQ=255)                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  2:1588404/139‑1 (MQ=255)                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  1:106036/139‑1 (MQ=255)                              tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                         >  2:1484974/1‑139 (MQ=255)                                gCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGtt                                                                                                       >  2:200485/1‑139 (MQ=255)                                 ccACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCa                                                                                                                 >  2:346094/1‑128 (MQ=255)                                 ccACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCa                                                                                                                 <  1:346094/128‑1 (MQ=255)                                 ccACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTg                                                                                                      <  2:310905/139‑1 (MQ=255)                                   aCGTACCGGCGAAACCTTTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTa                                                                                                    <  1:1646685/139‑1 (MQ=255)                                             gAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc                                                                                          <  2:443568/139‑1 (MQ=255)                                                  cATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                                                     <  1:302709/139‑1 (MQ=255)                                                           aaGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTgg                                                                            <  2:449592/139‑1 (MQ=255)                                                           aaGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTgg                                                                            <  1:722242/139‑1 (MQ=255)                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTAcg                                                                                                                      >  2:11488/1‑96 (MQ=255)                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTAcg                                                                                                                      <  1:11488/96‑1 (MQ=255)                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  2:433837/139‑1 (MQ=255)                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:200485/139‑1 (MQ=255)                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  2:325982/139‑1 (MQ=255)                                                             gCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc                                                                                          <  2:689176/123‑1 (MQ=255)                                                             gCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc                                                                                          >  1:689176/1‑123 (MQ=255)                                                             gCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTATGGGCGCGCGCAGGATTTTGACGGAAGCGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgcg                                                                          <  1:998149/139‑1 (MQ=38)                                                              ccGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgcgc                                                                         >  1:1115673/1‑139 (MQ=255)                                                                  ttAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCt                                                                     >  1:872914/1‑139 (MQ=255)                                                                   tAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCtt                                                                    >  2:1563893/1‑139 (MQ=255)                                                                   tAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCtt                                                                    >  1:1630044/1‑139 (MQ=255)                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                 <  2:1012193/139‑1 (MQ=255)                                                                               tCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg                                                                                                            >  1:723447/1‑87 (MQ=255)                                                                               tCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  2:723447/87‑1 (MQ=255)                                                                               tCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           >  2:135828/1‑120 (MQ=255)                                                                               tCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:135828/120‑1 (MQ=255)                                                                                cTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:757655/119‑1 (MQ=255)                                                                                cTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           >  2:757655/1‑119 (MQ=255)                                                                                 tGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAg                                                      <  1:865757/139‑1 (MQ=255)                                                                                 tGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAg                                                      <  1:1484974/139‑1 (MQ=255)                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTgg                                                                            >  2:1395943/1‑112 (MQ=255)                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTgg                                                                            <  1:1395943/112‑1 (MQ=255)                                                                                        aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTAcgccg                                                                                                                   <  2:1545173/71‑1 (MQ=39)                                                                                        aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTAcgccg                                                                                                                   >  1:1545173/1‑71 (MQ=39)                                                                                          cATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:563046/109‑1 (MQ=255)                                                                                          cATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           >  2:563046/1‑109 (MQ=255)                                                                                                  gggCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGa                                     <  1:133550/139‑1 (MQ=255)                                                                                                      gcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  1:1043963/64‑1 (MQ=25)                                                                                                      gcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg                                                                                                            >  2:1043963/1‑64 (MQ=25)                                                                                                      gcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg                                                                                                  <  2:1637141/74‑1 (MQ=37)                                                                                                      gcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg                                                                                                  >  1:1637141/1‑74 (MQ=37)                                                                                                      gcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCg                                 >  1:571164/1‑139 (MQ=255)                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGA‑TTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCt                               >  1:804338/1‑139 (MQ=25)                                                                                                         cgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCtt                              >  2:436037/1‑139 (MQ=255)                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                           <  1:1563893/139‑1 (MQ=255)                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                           <  2:571164/139‑1 (MQ=255)                                                                                                                    gACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATaa                   <  2:1115673/139‑1 (MQ=255)                                                                                                                      cGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATaaaa                 <  1:436037/139‑1 (MQ=255)                                                                                                                        gAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTAcgccg                                                                                                                   <  1:941574/39‑1 (MQ=18)                                                                                                                        gAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTAcgccg                                                                                                                   >  2:941574/1‑39 (MQ=18)                                                                                                                        gAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca               >  1:1541475/1‑139 (MQ=255)                                                                                                                         aaGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaacaa              >  1:261698/1‑139 (MQ=255)                                                                                                                           gagaCGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAAcc            <  2:973655/139‑1 (MQ=255)                                                                                                                            agaCGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCt           <  2:872914/139‑1 (MQ=255)                                                                                                                                   tttGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca               >  1:492845/1‑128 (MQ=255)                                                                                                                                   tttGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca               <  2:492845/128‑1 (MQ=255)                                                                                                                                     tGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCaa  >  1:536061/1‑139 (MQ=255)                                                                                                                                     tGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCaa  >  1:1168797/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                        TGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAA  >  NC_000913/247325‑247596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑