Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,385,857	C→T	*91* (TAG→TAA)	yhcO ←	putative barnase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,385,857	0	C	T	100.0%	197.7 / NA	62	*91* (TAG→TAA)	yhcO	putative barnase inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/24);  total (38/24)

AGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCA  >  NC_000913/3385726‑3385990                                                                                                                                    |                                                                                                                                        aGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGAc                                                                                                                                  >  1:1308816/1‑139 (MQ=255)  ggCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACg                                                                                                                                 <  2:1623311/139‑1 (MQ=255)       aaCGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACAt                                                                                                                            >  1:75313/1‑139 (MQ=255)         cGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCATTAATGACGAACATTa                                                                                                                          >  2:342748/1‑139 (MQ=255)              tACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACg                                                                                                                     >  2:193278/1‑139 (MQ=255)                ccAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCa                                                                                                                   <  1:46330/139‑1 (MQ=255)                ccAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCa                                                                                                                   <  2:75313/139‑1 (MQ=255)                        cTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑t                                                                                                          >  2:699510/1‑139 (MQ=255)                        cTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑t                                                                                                          >  1:1122003/1‑139 (MQ=255)                          gAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTaa                                                                                                                         <  1:177859/123‑1 (MQ=255)                          gAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTaa                                                                                                                         >  2:177859/1‑123 (MQ=255)                          gAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCACATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTc                                                                                                        >  2:1307415/1‑139 (MQ=255)                              cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGc                                                                                                    >  1:1567388/1‑139 (MQ=255)                               tCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGct                                                                                                   >  1:1381067/1‑139 (MQ=255)                                cGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACTCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGctc                                                                                                  >  2:665115/1‑139 (MQ=255)                                cGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCGGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACGTTAAAACGCAAATGCCC‑TTCCAGctc                                                                                                  >  2:1052627/1‑139 (MQ=255)                                        tGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTg                                                                                          <  2:755112/139‑1 (MQ=255)                                           cacCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCt                                                                                       <  1:779721/139‑1 (MQ=255)                                                     gCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAg                                                                            <  2:1567388/139‑1 (MQ=255)                                                      cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGc                                                                           >  1:380881/1‑139 (MQ=255)                                                      cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGc                                                                           >  1:554667/1‑139 (MQ=255)                                                           gCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGctc                                                                                                  <  1:145559/112‑1 (MQ=255)                                                           gCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGctc                                                                                                  >  2:145559/1‑112 (MQ=255)                                                               aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAAc                                                                  >  1:1026105/1‑139 (MQ=255)                                                                aCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCATTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAAcg                                                                 <  1:342748/139‑1 (MQ=255)                                                                 cTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAAcgc                                                                >  1:688328/1‑139 (MQ=255)                                                                         aTAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAAc                                                        >  2:434881/1‑139 (MQ=255)                                                                            aaCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGct                                                                                                   >  2:374694/1‑94 (MQ=255)                                                                            aaCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGct                                                                                                   <  1:374694/94‑1 (MQ=255)                                                                            aaCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGc                                                     >  1:813372/1‑139 (MQ=255)                                                                             aCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCg                                                    <  1:1017274/139‑1 (MQ=255)                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTCGTTTATTGAGAAAATTAAAAAGCCAATTCCCTTTCCAG‑TCTTTCTTTTCCTCCATAAAAAAGAAATATAACCCCCCAAAAACGGca                                                    >  2:148171/1‑138 (MQ=255)                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGc                                                                                         <  1:1567319/102‑1 (MQ=255)                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGc                                                                                         >  2:1567319/1‑102 (MQ=255)                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCt                                                                                       <  1:809046/104‑1 (MQ=255)                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCt                                                                                       >  2:809046/1‑104 (MQ=255)                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAAcg                                                                 <  1:623985/125‑1 (MQ=255)                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAAcg                                                                 >  2:623985/1‑125 (MQ=255)                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGa                                                   >  1:965721/1‑139 (MQ=255)                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGa                                                   >  1:565427/1‑139 (MQ=255)                                                                                   aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTg                                                                                          <  2:635650/96‑1 (MQ=255)                                                                                   aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTg                                                                                          >  1:635650/1‑96 (MQ=255)                                                                                   aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGt                                              >  2:898305/1‑139 (MQ=255)                                                                                   aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGt                                              >  2:296183/1‑139 (MQ=255)                                                                                         ttGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctc                                        >  1:817709/1‑139 (MQ=255)                                                                                            tCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTcc                                                                                                       >  1:139141/1‑74 (MQ=255)                                                                                            tCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTcc                                                                                                       <  2:139141/74‑1 (MQ=255)                                                                                                    tatTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGaa                             <  1:193278/139‑1 (MQ=255)                                                                                                      ttGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtttt                                           >  2:1532092/1‑123 (MQ=255)                                                                                                      ttGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtttt                                           <  1:1532092/123‑1 (MQ=255)                                                                                                      ttGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACa                           >  1:1563350/1‑139 (MQ=255)                                                                                                      ttGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACAGTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACa                           >  1:642656/1‑139 (MQ=255)                                                                                                          ccccGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAAtt                       <  2:1381067/139‑1 (MQ=255)                                                                                                            ccGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAg                                                                            >  2:59515/1‑84 (MQ=255)                                                                                                            ccGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAg                                                                            <  1:59515/84‑1 (MQ=255)                                                                                                             cGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCaa                    <  1:699510/139‑1 (MQ=255)                                                                                                              ggTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAAc                            <  1:922419/130‑1 (MQ=255)                                                                                                              ggTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAt                   >  2:466273/1‑139 (MQ=255)                                                                                                              ggTTCGGGGGCTTTTTTTAGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAAAAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAAc                            >  2:922419/1‑130 (MQ=255)                                                                                                                   gggggCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCaa              >  2:1364711/1‑139 (MQ=255)                                                                                                                        cTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGt         <  2:817709/139‑1 (MQ=255)                                                                                                                               ttGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCa  <  2:153748/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        AGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCC‑TTCCAGCTCTTCCTCTGCCT‑CATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCA  >  NC_000913/3385726‑3385990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑