| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,970,015 | T→C | W329R (TGG→CGG) | wzzE → | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,970,015 | 0 | T | C | 95.8% | 149.9 / ‑2.5 | 48 | W329R (TGG→CGG) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (25/21); total (26/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.50e-01 | |||||||||||
AGAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCA‑TTGTC‑GGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGAC > NC_000913/3969881‑3970146 | aGAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCgggg < 1:1388374/139‑1 (MQ=255) ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTc > 2:1159962/1‑139 (MQ=255) ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGATATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTc > 1:170220/1‑139 (MQ=255) ttAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTC‑ggggggg < 2:900072/139‑2 (MQ=255) ttAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTC‑ggggggg < 1:1543823/139‑2 (MQ=255) ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCgg > 1:1398342/1‑139 (MQ=255) ttGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGgtg > 2:869714/1‑139 (MQ=255) tCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGc > 1:16255/1‑139 (MQ=255) tCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACccgccg < 2:285266/139‑1 (MQ=255) gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCAtt < 2:1579874/127‑1 (MQ=255) gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCAtt > 1:1579874/1‑127 (MQ=255) cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc > 1:1003616/1‑139 (MQ=255) cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc > 1:1258696/1‑139 (MQ=255) cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc > 2:994763/1‑139 (MQ=255) gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt > 1:1312070/1‑139 (MQ=255) gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 1:1159962/139‑1 (MQ=255) aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑GCGATCGGGGCTGGTGTCGCCTTAACCCCCCCTTGGTCGGAAAaacaac > 1:962267/1‑139 (MQ=255) aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac > 2:489795/1‑139 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca < 2:539996/139‑1 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTC‑ggggggg > 1:560914/1‑88 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTC‑ggggggg < 2:560914/89‑2 (MQ=255) tGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa < 2:83916/121‑1 (MQ=255) tGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa > 1:83916/1‑121 (MQ=255) cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAt > 1:242249/1‑122 (MQ=255) cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAt < 2:242249/122‑1 (MQ=255) gTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTg > 2:352114/1‑139 (MQ=255) aCGGCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTC‑GGGGGGACTGATCGGGGCTGGTGTAGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAg > 2:1065479/1‑139 (MQ=255) aCGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAg > 1:1174937/1‑139 (MQ=255) cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCa < 2:1207600/96‑1 (MQ=255) cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCa > 1:1207600/1‑96 (MQ=255) ccGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCgggg > 1:811361/1‑59 (MQ=255) ccGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCgggg < 2:811361/59‑1 (MQ=255) aaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt < 2:1258696/139‑1 (MQ=255) gcgATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATTGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt < 2:1003616/139‑1 (MQ=255) aCGTCGTGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGaa > 2:840865/1‑139 (MQ=255) cgtGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCCGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa < 2:1174937/139‑1 (MQ=255) tGCCTTCCTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg > 1:104588/1‑139 (MQ=255) ttCCTGATGATTATGTGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGaa < 1:352114/139‑1 (MQ=255) tCCTGATGATTATGCGGGGCA‑TTGGCGGGGGGG‑CTGATCGGGGCGGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGaaa < 1:994763/139‑1 (MQ=255) ccTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 2:792350/69‑1 (MQ=255) ccTGATGATTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt > 1:792350/1‑69 (MQ=255) tgatgaTTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa < 1:1065479/139‑1 (MQ=255) gatTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc > 2:404984/1‑64 (MQ=255) gatTATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 1:404984/64‑1 (MQ=255) atTATGCGGGGCATTTGCG‑GGGGGG‑CTGATCGGGGCTGGGGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac < 2:170220/139‑1 (MQ=255) tATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAt > 2:277235/1‑66 (MQ=255) tATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAt < 1:277235/66‑1 (MQ=255) tATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg > 2:488141/1‑124 (MQ=255) tATGCGGGGCA‑TTGTCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg < 1:488141/124‑1 (MQ=255) | AGAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCA‑TTGTC‑GGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGAC > NC_000913/3969881‑3970146 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |