| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,508,454 | Δ1 bp | coding (137/879 nt) | php ← | phosphotriesterase homology protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,508,451 | 0 | C | . | 98.2% | 214.6 / ‑2.1 | 54 | coding (140/879 nt) | php | phosphotriesterase homology protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base . (27/26); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAA > NC_000913/3508319‑3508583 | tCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCgggt > 1:1316516/1‑138 (MQ=255) gggAAAAACGCGTCTTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGtcat > 2:1324634/1‑139 (MQ=255) aaCGCGTCCTGGTAATAACC‑GTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCg < 2:102979/139‑1 (MQ=255) gTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCACCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCt > 1:1488029/1‑139 (MQ=255) gTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTc < 2:462106/122‑1 (MQ=255) gTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTc > 1:462106/1‑122 (MQ=255) gTACAGGCCACCACGTTGATCCCCGTCTCGCGCATT‑CATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc < 2:479767/139‑1 (MQ=255) tACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc < 1:1196131/139‑1 (MQ=255) gCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGtcat < 2:167555/106‑1 (MQ=255) gCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGtcat > 1:167555/1‑106 (MQ=255) gTCTCGCGCATTACATCATGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTc > 2:836428/1‑139 (MQ=255) gTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTc > 1:35388/1‑139 (MQ=255) tctcGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCa < 2:502105/135‑1 (MQ=255) tctcGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCa > 1:502105/1‑135 (MQ=255) gcgcATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGcccgg > 2:867409/1‑76 (MQ=255) gcgcATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGcccgg < 1:867409/78‑3 (MQ=255) gcgcATTAAATCAAGCATAAATTGCTCATTGCGCCCCATGTAACGGTCCGTCATCTCAATCACATTACGCACG‑CCCTGGTCATCAGGTCGTTCATCTCCTTGCAAATGTACGCATACTGATCAAGGCGGCAGTCCACgt < 2:1488029/139‑1 (MQ=255) cgcATTACATCAAGCATAAATTGCGCTTTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACgtt > 1:1083197/1‑139 (MQ=255) aCATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAgg < 2:845143/80‑1 (MQ=255) aCATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAgg > 1:845143/1‑80 (MQ=255) aGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGcc > 1:224875/1‑139 (MQ=255) gCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCg < 2:1373798/139‑1 (MQ=255) aTAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc < 1:483542/95‑1 (MQ=255) aTAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc > 2:483542/1‑95 (MQ=255) aTAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCa < 1:694119/115‑1 (MQ=255) aTAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCa > 2:694119/1‑115 (MQ=255) aaaTTGCGCTTTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTAATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGgaga < 2:1083197/139‑1 (MQ=255) ttgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATg > 2:767077/1‑139 (MQ=255) tgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGcccg > 2:78100/1‑47 (MQ=255) tgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGcccg < 1:78100/48‑2 (MQ=255) tgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATg < 2:432813/79‑1 (MQ=255) tgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATg > 1:432813/1‑79 (MQ=255) cgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAg < 2:35388/139‑1 (MQ=255) aCGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCgg < 2:1138746/112‑1 (MQ=255) aCGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCgg > 1:1138746/1‑112 (MQ=255) aCGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGga < 2:264015/113‑1 (MQ=255) aCGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGga > 1:264015/1‑113 (MQ=255) ttGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCAt > 1:109788/1‑69 (MQ=255) ttGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCAt < 2:109788/69‑1 (MQ=255) ttGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAg > 1:469508/1‑139 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa > 2:2501/1‑58 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa < 1:2501/58‑1 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc > 2:844169/1‑63 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc < 1:844169/63‑1 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAata > 1:5073/1‑117 (MQ=255) ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAata < 2:5073/117‑1 (MQ=255) gTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCAt < 2:806704/46‑1 (MQ=39) gTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCAt > 1:806704/1‑46 (MQ=39) gTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTc > 1:615707/1‑127 (MQ=255) gTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTc < 2:615707/127‑1 (MQ=255) gTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGgt > 1:562174/1‑139 (MQ=255) ctcAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa < 1:1042377/52‑1 (MQ=255) ctcAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa > 2:1042377/1‑52 (MQ=255) cAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAgg > 1:1016327/1‑70 (MQ=255) cAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAgg < 2:1016327/70‑1 (MQ=255) aTCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGt < 2:224875/139‑1 (MQ=255) aTTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGAt < 2:1316516/139‑1 (MQ=255) ttACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAAAGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATc > 2:111316/1‑139 (MQ=255) acgcacg‑cCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCaa < 1:1324634/139‑1 (MQ=255) acgcacg‑cCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCaa < 1:767077/139‑1 (MQ=255) cgcacg‑cCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCaa < 1:1108564/138‑1 (MQ=255) cgcacg‑cCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCaa > 2:1108564/1‑138 (MQ=255) | TCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAA > NC_000913/3508319‑3508583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |