| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,606,094 | (G)8→9 | noncoding (72/87 nt) | leuV ← | tRNA‑Leu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,606,086 | 1 | . | G | 91.5% | 148.4 / 5.9 | 47 | noncoding (80/87 nt) | leuV | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base G (22/21); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG > NC_000913/4605978‑4606208 | ccATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGTATCAGGGATGAAAAATCAAGGAAGTAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑g > 1:921934/1‑110 (MQ=255)ccATCGTGCCTGGCACTTATTGCTGGCATCAAGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑g < 2:921934/110‑1 (MQ=255) tCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑ggggggggg > 1:1088484/1‑114 (MQ=255) tCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑ggggggggg < 2:1088484/115‑2 (MQ=255) gTGCCTGGCACTTATTGCTGGCATCATTCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGATGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑GGGGGGGGTACTTGAAcccc < 1:586438/124‑1 (MQ=255) gTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAAcccc > 2:586438/1‑124 (MQ=255) tGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTa > 2:809337/1‑133 (MQ=255) tGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTa < 1:809337/133‑1 (MQ=255) gCACTTATTGCTGGCATCATGCTGATGATGATGAATTTGAATCAGTGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑ggggg > 2:869609/1‑102 (MQ=255) gCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAaca > 2:460385/1‑139 (MQ=255) gCACTTATTGCTGGCATCATGCTGATGATAAGGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑ggggg < 1:869609/102‑1 (MQ=255) tATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGa > 2:199388/1‑139 (MQ=255) aTTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCa < 2:1369489/113‑1 (MQ=255) aTTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCa > 1:1369489/1‑113 (MQ=255) ttGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCa > 1:1339526/1‑112 (MQ=255) ttGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCa < 2:1339526/112‑1 (MQ=255) tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCAc < 2:1065461/104‑1 (MQ=255) tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCAc > 1:1065461/1‑104 (MQ=255) tGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa < 1:938730/139‑1 (MQ=255) cTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc < 1:1024356/139‑1 (MQ=255) aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCa > 2:355314/1‑90 (MQ=255) aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCa < 1:355314/90‑1 (MQ=255) aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc > 1:312046/1‑139 (MQ=255) gAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc < 1:199388/139‑1 (MQ=255) tGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAAcccc < 1:1220865/80‑1 (MQ=255) tGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAAcccc > 2:1220865/1‑80 (MQ=255) taaTCAGGGAATAAAAATCAAGGAAGAAACAAAAAAGGAAGTAAAGATAATTGGTGCCAGG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg < 2:918198/138‑1 (MQ=18) aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca > 2:412044/1‑139 (MQ=255) tCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcaca < 1:460385/139‑1 (MQ=255) tCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcaca < 1:412044/139‑1 (MQ=255) aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTaa > 2:1099791/1‑94 (MQ=255) aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTaa < 1:1099791/94‑1 (MQ=255) gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCa < 1:209728/70‑1 (MQ=255) gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCa > 2:209728/1‑70 (MQ=255) tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTaa > 2:1290959/1‑82 (MQ=255) tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTaa < 1:1290959/82‑1 (MQ=255) aagaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcaca < 2:365890/118‑1 (MQ=255) aagaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcaca > 1:365890/1‑118 (MQ=255) aaCAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAg > 2:313375/1‑64 (MQ=255) aaCAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAg < 1:313375/64‑1 (MQ=255) aaCAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa > 1:1231483/1‑96 (MQ=255) aaCAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa < 2:1231483/96‑1 (MQ=255) aCAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc < 2:312046/139‑1 (MQ=255) aGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTGATATCGCCTCGTTTg > 1:1258141/1‑139 (MQ=255) ggAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc > 2:455052/1‑94 (MQ=255) ggAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 1:455052/94‑1 (MQ=255) gAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAAcccc > 1:88695/1‑42 (MQ=21) gAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAAcccc < 2:88695/42‑1 (MQ=21) gTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa < 1:1328571/83‑1 (MQ=255) gTAAAGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa > 2:1328571/1‑83 (MQ=255) aaGATAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAg > 1:596582/1‑139 (MQ=255) tAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCg < 2:927818/119‑1 (MQ=255) tAATTGGTGC‑‑GAGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCg > 1:927818/1‑119 (MQ=255) | CCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG > NC_000913/4605978‑4606208 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |