Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,450,392	C→T	A320T (GCA→ACA)	tynA ←	tyramine oxidase, copper‑requiring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,450,392	0	C	T	100.0%	175.5 / NA	55	A320T (GCA→ACA)	tynA	tyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/28);  total (27/28)

TTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCA  >  NC_000913/1450256‑1450504                                                                                                                                          |                                                                                                                 ttCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTgg                                                                                                                >  1:1255304/1‑139 (MQ=255)      gCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCAtt                                                                                                           >  2:586482/1‑139 (MQ=255)                     ccAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTAcc                                                                                            <  2:1508858/139‑1 (MQ=255)                              ccAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTAcc                                                                                            >  1:1342908/1‑130 (MQ=255)                              ccAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTAcc                                                                                            <  2:1342908/130‑1 (MQ=255)                                        atatCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTaa                                                                         <  2:490737/139‑1 (MQ=255)                                           tCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGGTTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGAcc                                                                                       <  1:802366/122‑1 (MQ=255)                                           ccGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGAcc                                                                                       >  2:802366/2‑122 (MQ=255)                                            cGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAt                                                                     >  1:217538/1‑139 (MQ=255)                                              ccAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAtt                                                                    >  2:979693/1‑138 (MQ=255)                                              ccAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAtt                                                                    <  1:979693/138‑1 (MQ=255)                                                  tAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAttttttt                                                               <  1:786606/139‑1 (MQ=255)                                                  tAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAttttttt                                                               <  2:462416/139‑1 (MQ=255)                                                          tAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAttttttt                                                               <  1:412248/131‑1 (MQ=255)                                                          tAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAttttttt                                                               >  2:412248/1‑131 (MQ=255)                                                          tAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTa                                                       >  2:1030099/1‑139 (MQ=255)                                                                 tACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAAc                                                >  2:1122760/1‑139 (MQ=255)                                                                  aCCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACg                                               >  2:150809/1‑139 (MQ=255)                                                                      tCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTGTCTGTTCTAAAtatgtctctt                                           >  1:888603/1‑130 (MQ=255)                                                                        aGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTc                                                              >  2:556943/1‑118 (MQ=255)                                                                        aGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTc                                                              <  1:556943/118‑1 (MQ=255)                                                                        aGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGcc                                         <  1:1030099/139‑1 (MQ=255)                                                                          gCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGccac                                       <  2:364028/139‑1 (MQ=255)                                                                          gCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGccac                                       >  1:364028/1‑139 (MQ=255)                                                                               aTGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGt                                  >  1:21202/1‑139 (MQ=255)                                                                                    ttGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAAATGGGCGTGTGGTCATTGGCACCGGAGCTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGAc                                             <  2:872318/124‑1 (MQ=255)                                                                                    ttGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGAc                                             >  1:872318/1‑124 (MQ=255)                                                                                    ttGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTAcc                                                                                            >  1:519613/1‑76 (MQ=255)                                                                                    ttGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTAcc                                                                                            <  2:519613/76‑1 (MQ=255)                                                                                    ttGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCg                             <  2:1255304/139‑1 (MQ=255)                                                                                     tGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGa                            >  1:707686/1‑139 (MQ=255)                                                                                     tGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGa                            >  2:1352852/1‑139 (MQ=255)                                                                                      gCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGAt                           <  2:21202/139‑1 (MQ=255)                                                                                          aGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGa                       <  2:217538/139‑1 (MQ=255)                                                                                            gCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGttt                                <  1:1324351/128‑1 (MQ=255)                                                                                            gCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGttt                                >  2:1324351/1‑128 (MQ=255)                                                                                            gCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGa                            <  2:1165256/132‑1 (MQ=255)                                                                                            gCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGa                            >  1:1165256/1‑132 (MQ=255)                                                                                                 aCTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTAcc                                                                                            <  2:674771/63‑1 (MQ=255)                                                                                                 aCTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTAcc                                                                                            >  1:674771/1‑63 (MQ=255)                                                                                                 aCTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAttttttt                                                               <  1:1274028/92‑1 (MQ=255)                                                                                                 aCTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAttttttt                                                               >  2:1274028/1‑92 (MQ=255)                                                                                                      cGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTAcc                                                                                            <  1:114993/58‑1 (MQ=255)                                                                                                      cGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTAcc                                                                                            >  2:114993/1‑58 (MQ=255)                                                                                                      cGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTaa                                                                         <  1:1503180/77‑1 (MQ=255)                                                                                                      cGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTaa                                                                         >  2:1503180/1‑77 (MQ=255)                                                                                                      cGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCagtag           >  2:800970/1‑139 (MQ=255)                                                                                                           cAACGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGTCCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGcc      <  1:800970/139‑1 (MQ=255)                                                                                                             aCGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCAt    <  1:1292527/139‑1 (MQ=255)                                                                                                             aCGCGGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCAt    <  1:586482/139‑1 (MQ=255)                                                                                                              cgcgGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTg                                                            <  2:582726/82‑1 (MQ=255)                                                                                                              cgcgGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTg                                                            >  1:582726/1‑82 (MQ=255)                                                                                                               gcgGTCACGGCCATC‑AAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCa  <  2:707686/139‑1 (MQ=255)                                                                                                                                      cGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTaa                                                                         <  1:1363172/46‑1 (MQ=255)                                                                                                                                      cGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTaa                                                                         >  2:1363172/1‑46 (MQ=255)                                                                                                                                          |                                                                                                                 TTCATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATC‑AAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCA  >  NC_000913/1450256‑1450504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑