Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,214,339	(C)6→5	coding (306/1686 nt)	yehU ←	inner membrane putative sensory kinase in two‑component system with YehT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,214,334	0	C	.	100.0%	155.9 / NA	38	coding (311/1686 nt)	yehU	inner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (18/20);  total (18/20)

GACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACC  >  NC_000913/2214198‑2214463                                                                                                                                         |                                                                                                                                  gacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                 <  2:665054/139‑1 (MQ=255) gacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                 <  1:253070/139‑1 (MQ=255) gacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCAGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                 <  1:570698/139‑1 (MQ=255)     aaCGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccat                                                                                                                             >  2:555012/1‑137 (MQ=255)      aCGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccatc                                                                                                                            <  2:979821/139‑4 (MQ=255)                            ggggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                     <  2:1016081/139‑1 (MQ=255)                            ggggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                     <  2:544407/139‑1 (MQ=255)                            ggggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                     <  2:418468/139‑1 (MQ=255)                                          tATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGc                                                                                       >  1:250985/1‑139 (MQ=255)                                                tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcg                                                                                 >  1:359381/1‑139 (MQ=255)                                                tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcg                                                                                 >  2:362651/1‑139 (MQ=255)                                                tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcg                                                                                 >  2:1245295/1‑139 (MQ=255)                                                  cgcCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                 >  2:224326/1‑90 (MQ=255)                                                  cgcCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                 <  1:224326/90‑1 (MQ=255)                                                    cccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                >  1:363760/1‑120 (MQ=255)                                                    cccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                <  2:363760/120‑1 (MQ=255)                                                    cccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcgacga                                                                             >  1:116895/1‑139 (MQ=255)                                                        cggcggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccgg                                                                         >  2:977987/1‑139 (MQ=255)                                                         ggcggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccgga                                                                        <  1:20222/139‑1 (MQ=255)                                                           cggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggacc                                                                      <  1:722139/139‑1 (MQ=255)                                                                     tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                            <  1:512002/139‑1 (MQ=255)                                                                          tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccat                                                                                                                             >  1:587886/1‑68 (MQ=255)                                                                          tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccat                                                                                                                             <  2:587886/70‑3 (MQ=255)                                                                          tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                                                       >  2:922341/1‑139 (MQ=255)                                                                                 ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGc                                                                                       <  1:545196/100‑1 (MQ=255)                                                                                 ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGc                                                                                       >  2:545196/1‑100 (MQ=255)                                                                                    cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgcg                                             >  1:499856/1‑139 (MQ=255)                                                                                      ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGccc                                                      >  1:680139/1‑128 (MQ=255)                                                                                      ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGccc                                                      <  2:680139/128‑1 (MQ=255)                                                                                         aGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTa                                                              <  2:240584/117‑1 (MQ=255)                                                                                         aGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTa                                                              >  1:240584/1‑117 (MQ=255)                                                                                             aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                    <  2:499856/139‑1 (MQ=255)                                                                                             aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                    <  2:832712/139‑1 (MQ=255)                                                                                             aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                    <  1:189638/139‑1 (MQ=255)                                                                                                  tCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGt                               <  1:555012/139‑1 (MQ=255)                                                                                                     aCGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTAtt                            <  2:737423/139‑1 (MQ=255)                                                                                                       gATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTgg                          <  1:977987/139‑1 (MQ=255)                                                                                                         tGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCa                        >  2:1079696/1‑139 (MQ=255)                                                                                                                gaTCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                            <  2:1119688/96‑1 (MQ=255)                                                                                                                gaTCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                            >  1:1119688/1‑96 (MQ=255)                                                                                                                     tGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa            >  2:889825/1‑139 (MQ=255)                                                                                                                          cTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg       <  1:922341/139‑1 (MQ=255)                                                                                                                             aaCGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                           <  2:59868/52‑1 (MQ=255)                                                                                                                             aaCGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                           >  1:59868/1‑52 (MQ=255)                                                                                                                               cgcgGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAAcc  >  1:563444/1‑139 (MQ=255)                                                                                                                                    tCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTa                                         <  1:352785/95‑1 (MQ=255)                                                                                                                                    tCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTa                                         >  2:352785/1‑95 (MQ=255)                                                                                                                                         |                                                                                                                                  GACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACC  >  NC_000913/2214198‑2214463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑