| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,854,733 | (T)5→4 | coding (396/2079 nt) | fhlA → | formate hydrogenlyase transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,854,729 | 0 | T | . | 100.0% | 198.7 / NA | 48 | coding (392/2079 nt) | fhlA | formate hydrogenlyase transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (21/27); total (21/27) | |||||||||||
AGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTC > NC_000913/2854593‑2854850 | agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttt > 2:438061/1‑139 (MQ=255) tGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGc < 2:1191557/139‑1 (MQ=255) gTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCt < 1:851882/139‑1 (MQ=255) tCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCtg < 2:1247436/139‑1 (MQ=255) gATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTAt < 1:260065/139‑1 (MQ=255) tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTAt > 1:1239070/1‑139 (MQ=255) cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGa > 2:130683/1‑139 (MQ=255) cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGa > 1:1234113/1‑139 (MQ=255) ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttttggt > 2:68967/1‑111 (MQ=255) ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttttggt < 1:68967/114‑4 (MQ=255) ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACg < 2:921483/139‑1 (MQ=255) ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACg > 1:1154419/1‑139 (MQ=255) gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt < 2:1234113/139‑1 (MQ=255) gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt < 1:967917/139‑1 (MQ=255) tgAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttttgg > 2:963844/1‑90 (MQ=255) tgAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttttgg < 1:963844/92‑3 (MQ=255) ccTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt > 2:1079378/1‑115 (MQ=255) ccTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt < 1:1079378/115‑1 (MQ=255) ggCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAAt > 2:770851/1‑139 (MQ=255) gCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGc > 2:910176/1‑139 (MQ=255) cTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCa < 2:1154419/139‑1 (MQ=255) gACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAAc > 2:288481/1‑139 (MQ=255) aCGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACa < 1:130683/139‑1 (MQ=255) aGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt > 1:757724/1‑96 (MQ=255) aGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt < 2:757724/96‑1 (MQ=255) ggTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGTAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTGATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTAc < 2:1239070/139‑1 (MQ=255) ggCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAg < 1:288481/139‑1 (MQ=255) cTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGAt > 1:780518/1‑139 (MQ=255) ttGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgtcgtc < 1:438061/139‑1 (MQ=255) tGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCa < 1:910176/139‑1 (MQ=255) ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc > 1:525542/1‑139 (MQ=255) ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc < 1:883666/139‑1 (MQ=255) ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTACTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc < 2:806001/139‑1 (MQ=255) tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt > 1:613594/1‑58 (MQ=255) tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt < 2:613594/58‑1 (MQ=255) tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGccc < 2:939682/63‑1 (MQ=255) tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGccc > 1:939682/1‑63 (MQ=255) tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGt < 1:365679/88‑1 (MQ=255) tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc > 1:685766/1‑139 (MQ=255) tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc > 1:418182/1‑139 (MQ=255) tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc > 2:731447/1‑139 (MQ=255) tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc > 2:975530/1‑139 (MQ=255) tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCATTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGt > 2:365679/1‑88 (MQ=255) gCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGcc < 2:1127337/139‑1 (MQ=255) gCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGcc < 1:975530/139‑1 (MQ=255) cACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgc < 2:685766/139‑1 (MQ=255) tGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgtc < 2:988339/139‑1 (MQ=255) ggcggAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt < 2:829513/48‑1 (MQ=255) ggcggAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt > 1:829513/1‑48 (MQ=255) ggcggAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc < 2:846752/113‑1 (MQ=255) ggcggAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc > 1:846752/1‑113 (MQ=255) ggAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt < 2:619260/45‑1 (MQ=255) ggAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt > 1:619260/1‑45 (MQ=255) | AGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTC > NC_000913/2854593‑2854850 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |