Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,508,454	Δ1 bp	coding (137/879 nt)	php ←	phosphotriesterase homology protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,508,451	0	C	.	100.0%	228.3 / NA	55	coding (140/879 nt)	php	phosphotriesterase homology protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (29/26);  total (29/26)

CGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAA  >  NC_000913/3508332‑3508583                                                                                                                        |                                                                                                                                     cgTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTc                                                                                                                  <  1:939626/139‑1 (MQ=255)              aCCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGATCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa                                                                                                     <  2:455001/139‑1 (MQ=255)                  gTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGaa                                                                                                 >  2:1182152/1‑139 (MQ=255)                   tACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                >  2:622243/1‑139 (MQ=255)                   tACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                <  1:194768/139‑1 (MQ=255)                      aGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa                                                                                                     <  2:312564/131‑1 (MQ=255)                      aGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa                                                                                                     >  1:312564/1‑131 (MQ=255)                        gCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATa                                                                                           >  2:887212/1‑139 (MQ=255)                             cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATATTGAt                                                                                      >  1:748999/1‑139 (MQ=255)                                  tGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAgg                                                                                 >  1:676485/1‑139 (MQ=255)                                  tGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAgg                                                                                 >  2:43121/1‑139 (MQ=255)                                      ccccGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAggcggc                                                                             <  2:445227/139‑1 (MQ=255)                                       cccGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                >  1:1235217/1‑119 (MQ=255)                                       cccGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                <  2:1235217/119‑1 (MQ=255)                                             tcGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGTTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCAc                                                                      <  1:1203254/139‑1 (MQ=255)                                              cgcgCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACg                                                                     >  1:401181/1‑139 (MQ=255)                                                cgcATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                <  2:1172963/110‑1 (MQ=255)                                                cgcATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                >  1:1172963/1‑110 (MQ=255)                                                cgcATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACgtt                                                                   >  1:331685/1‑139 (MQ=255)                                                cgcATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACgtt                                                                   >  2:34545/1‑139 (MQ=255)                                                     tACATCAAGCATAAATTCCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGcccgg                                                                                                                                  <  2:224946/72‑3 (MQ=255)                                                     tACATCAAGCATAAATTCCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGcccgg                                                                                                                                  >  1:224946/1‑70 (MQ=255)                                                       cATCAAGCATAAATTGCGCATTGCGCCCCGTGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTa                                                            <  1:887212/139‑1 (MQ=255)                                                             gCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATg                                                                                                   <  1:459724/94‑1 (MQ=255)                                                             gCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATg                                                                                                   >  2:459724/1‑94 (MQ=255)                                                             gCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                >  1:1226821/1‑97 (MQ=255)                                                             gCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                <  2:1226821/97‑1 (MQ=255)                                                             gCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCg                                                      <  1:43121/139‑1 (MQ=255)                                                             gCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCg                                                      >  2:954834/1‑139 (MQ=255)                                                              cATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCgg                                                     <  2:401181/139‑1 (MQ=255)                                                                 aaaTTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGgaga                                                  <  1:34545/139‑1 (MQ=255)                                                                   attgcgcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGgagaga                                                >  1:454499/1‑139 (MQ=255)                                                                       cgcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                <  1:1240934/87‑1 (MQ=255)                                                                       cgcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                >  2:1240934/1‑87 (MQ=255)                                                                       cgcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCa                                                                                             <  2:220473/90‑1 (MQ=255)                                                                       cgcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCa                                                                                             >  1:220473/1‑90 (MQ=255)                                                                        gcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAat                                           >  1:540803/1‑139 (MQ=255)                                                                            tgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGc                                       >  1:1159299/1‑139 (MQ=255)                                                                              cgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAg                                     <  2:1159299/139‑1 (MQ=255)                                                                               gcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAg                                                                                                                         <  1:913677/54‑1 (MQ=255)                                                                               gcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAg                                                                                                                         >  2:913677/1‑54 (MQ=255)                                                                                           ggTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                        <  2:676485/139‑1 (MQ=255)                                                                                           ggTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                        <  2:540803/139‑1 (MQ=255)                                                                                           ggTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                        <  2:337285/139‑1 (MQ=255)                                                                                             ttGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTc                                                                                                                  >  2:705695/1‑47 (MQ=39)                                                                                             ttGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTc                                                                                                                  <  1:705695/47‑1 (MQ=39)                                                                                             ttGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAg                      >  2:1172298/1‑139 (MQ=255)                                                                                               ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGAt                                               >  2:1010590/1‑112 (MQ=255)                                                                                               ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGAt                                               <  1:1010590/112‑1 (MQ=255)                                                                                               ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAggg                    >  2:391266/1‑139 (MQ=255)                                                                                                    tctcAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                        >  1:1106961/1‑130 (MQ=255)                                                                                                    tctcAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                        <  2:1106961/130‑1 (MQ=255)                                                                                                       cAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATa                                                                                           >  1:899511/1‑60 (MQ=255)                                                                                                       cAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATa                                                                                           <  2:899511/60‑1 (MQ=255)                                                                                                           cacaTTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCg        <  1:383237/139‑1 (MQ=255)                                                                                                               ttACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATc    >  2:1049702/1‑139 (MQ=255)                                                                                                               ttACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGAAGTACACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATCCTCATGGGCGAGGGTGTAACCCGTCGGATc    >  2:713739/1‑139 (MQ=255)                                                                                                                 acgcacg‑cCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCaa  <  1:622243/139‑1 (MQ=255)                                                                                                                        |                                                                                                                                     CGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAA  >  NC_000913/3508332‑3508583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑